B09_Amd_0002_SOW_revised_0002.pdf

140P8524Q0002 Amd 0002 Chronic Toxicity Testing for Yosemite NP Page 1 of 2

Statement of Work

CHRONIC TOXICITY TESTING

Yosemite National Park

Objective:

Obtain accredited laboratory in California to perform Chronic Toxicity Testing to comply with state waste discharge permit requirements, for El Portal Wastewater Treatment Facility, Order Number R5-2019-0069, NPDES Number CA0081759.

Background:

Yosemite National Park operates the El Portal Wastewater Treatment Plant (EPWWTP) in El Portal, CA,on Hwy 140, just west of the Yosemite National Park boundary. EPWWTP is a one

(1) million gallon per day wastewater treatment facility that treats domestic waste from Yosemite Valley and the town of El Portal. The heaviest flows usually occur from April through October of any given year, when park visitation is at the highest.

Annual (one time per calendar year) Chronic Toxicity Testing is required by the California Regional Water Quality Control Board (RWQCB) to analyze the effluent that is discharged to percolation ponds. EPWWTP is in need of this test to satisfy permit requirements.

EPWWTP has an accredited Environmental Laboratory that is certified by the State of California. The scope of the certificate is limited to the “Fields of Testing” which accompany this Certificate. EPWWTP laboratory is not authorized to perform this test and must depend on an outside source to comply with waste discharge permit requirements. Order Number R5-2019- 0069, NPDES Number CA0081759.

Requirement:

National Park Service (NPS) will collect all necessary samples on site, not the responsibility of the laboratory. NPS will collect samples in June or July, yearly, weather dependent.

NPS will either ship samples in appropriate form to maintain set temperature limitations and hold time limitations to accredited laboratory or drive samples directly to the accredited laboratory if unable to maintain set temperature limitations or hold time limitations. See Note below.

Laboratory shall verify the chain of custody order that will accompany the samples to ensure accuracy. If samples arrive or are delivered outside the established temperature and time limitations, the laboratory will notify the NPS and it will be the responsibility of the NPS to resample.

Laboratory shall perform all Chronic Toxicity Testing per EPA methods, EPA-821-R-02-013.

NOTE: Per RWQCB permit, collected samples must be collected, transported and received by the lab within 36 hours of sample collection and must stay within 0-6 Celsius during hold time.

140P8524Q0002 Amd 0002 Chronic Toxicity Testing for Yosemite NP Page 2 of 2

Any variance of these above limitations will require resampling and resubmission of samples by the NPS to the accredited lab.

Laboratory Testing required:

Using U.S EPA Standard EPA-821-R-02-013

Chronic Toxicity Testing (Control + 5 effluent concentrations)

Quantity 1 Each-EPA 1000.0 7-day Survival and growth chronic test with fathead minnows Concurrent reference toxicant test w/fathead minnows Quantity 1 Each-EPA 1002.0 7-Day survival and reproduction chronic test with Ceriodaphnia dubia Concurrent reference toxicant test w/Ceriodaphnia dubia Quantity 1 Each-EPA 1003.0 96-hr. algal growth test w/Selenastrum capricornutum Concurrent reference toxicant test w/ Selenastrum capricornutum

Delivery:

For samples for EPWWTP, laboratory shall submit testing finding results directly to El Portal Wastewater Treatment Facility Laboratory Director both by email and with a hard copy sent by mail. Contact information will be provided upon award. Results shall be received no later than 14 business days from the date of sample receipt.