Sol_140G0326R0005_Amd_0001.pdf

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

(a) By completing items 8 and 15, and returning or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

E. IMPORTANT: is not is required copies to the issuing office.

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT 1. CONTRACT ID CODE

2. AMENDMENT/MODIFICATION NUMBER 3. EFFECTIVE DATE 4. REQUISITION/PURCHASE REQUISITION NUMBER 5. PROJECT NUMBER (If applicable)

7.ADMINISTERED BY (If other than Item 6) CODE

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

FACILITY CODE

9A. AMENDMENT OF SOLICITATION NUMBER

9B. DATED (SEE ITEM 11)

10A. MODIFICATION OF CONTRACT/ORDER NUMBER

10B. DATED (SEE ITEM 13)

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

The above numbered solicitation is amended as set forth in Item 14. is extended. is not extended.

12. ACCOUNTING AND APPROPRIATION DATA (If required) copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted;

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NUMBER AS DESCRIBED IN ITEM 14.

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER

NUMBER IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15C. DATE SIGNED

15A. NAME AND TITLE OF SIGNER (Type or print)

16C. DATE SIGNED

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

PAGE

6. ISSUED BY CODE

8. NAME AND ADDRESS OF CONTRACTOR (Number, street, county, State and ZIP Code) (X)

CODE

15B. CONTRACTOR/OFFEROR

(Signature of person authorized to sign)

16B. UNITED STATES OF AMERICA

(Signature of Contracting Officer)

Previous edition unusable

PAGESOF

Contractor

The hour and date specified for receipt of Offers to sign this document and return

Elizabeth Adam

See continuation page

USGS OAG SACRAMENTO ACQUISITION BR.

MODOC HALL, CSUS

3020 STATE UNIVERSITY DRIVE EAST

SACRAMENTO CA 95819-6027

GSB

140G0326R0005

08/31/2026

08/31/2026

1 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

140G0326R0005/0001

The purpose of this amendment is to answer vendor questions. The period for questions is closed, and no further questions will be accepted. The due date for quotes remains the same, September 8, 2026, at 10:00am PDT.

1. How many data entries are currently in the database? approximately 3000

2. How many samples need to be added to the database from recent years? How many samples per year are being added Approximately 400. The number per year is not a set number but varies depending on field events and how many virus isolates our fish health partners to send in for genotyping.

3. Shall the previously used database stay in place? Or should the deliverables include a plan for long term database plan which may include development of a new structure/system?

At this point we are aiming to update the current in-house excel database that has been in place for many years. That database will continue to be our main source of curated information. Future plans for any new system are desirable but beyond the financial scope of this contract.

4. Where (what lab) is the genotyping data being produced currently? The majority of genotyping is done at our lab, the USGS Western

Fisheries Research Center, where the genotyping program originated and has been hosted since the 1990s. We also occasionally get isolate metadata and genotyped sequences from partners at the Northwest Indian Fish Commission and

Oregon Department fo Fish and Wildlife.

5. What technology is being used for that?

Microarray? Sanger sequencing? Next generation sequencing? Other? RNA is extracted from each virus isolate and a fragment containing the midG sequence is generated by

PCR. The genotype consensus sequences are generated by Sanger or NGS either in-house or by sending the PCR fragement to a commercial sequencing service. Metadata is tracked using the in-house database that also contains the sequence generated. and the designated genotype number.

Continued...

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

140G0326R0005/0001

6. What is the size of each genotype that will be added or produced? Is it the full IHNV sequence or just select regions? IHNV genotypes have been established in North

America using a 303 nt mid-G region that is a variable region in the virus glycoprotein (G) gene. This short region has proven robust in comparison with phylogenies based on complete G genes (1610 nt) or full genomes ( ~ 11,132 nt).

It is the only sequence that facilitates phylogenetic analyses with archive of genotype data for over 3000 virus isolates that represent IHNV in all of North America.

7. What database engine does the current database use (e.g., PostgreSQL, mySQL, etc.)?

The current database is a large excel file containing all metadata associated with isolates and the genotyped sequence that is associated with each isolate.

8. How many WFRC staff need to be trained annually to conduct the IHNV typing? Once staff are trained they do not need to be trained annually but as needed when any changes in the system need to be implemented. Optimally there should be two staff members who are trained to conduct the IHNV genotyping.

Amendment POC:

Elizabeth Adam elizabeth_adam@ios.doi.gov

Sponsored by GSA FAR (48 CFR) 53.110