SOW_140G0325Q0194_revised_0001.docx
DOCX document 29 KB Posted
- Attached to
- DNA Extraction Cyanobacteria Federal contract opportunity
- Solicitation number
- 140G0325Q0194
About this file
This Statement of Work (SOW) details a contract for DNA analysis of cyanobacteria samples for the U.S. Geological Survey California Water Science Center. The project involves collecting and analyzing 100 samples from five monitoring stations in the San Francisco Bay Delta area between August 2025 and October 31, 2026. The contractor will be required to extract DNA from filters and perform quantitative PCR (qPCR) analyses on specific genes related to cyanotoxin biosynthesis (mcyE, cpcB, anaC, and sxtA), with data to be delivered in XLS, CSV, or TXT files by December 31, 2026.
Key technical requirements include maintaining chains-of-custody for laboratory operations, archiving DNA extracts for future sequencing, and meeting specific expertise qualifications. The laboratory leader must hold a Ph.D. in a field related to cyanobacterial ecology, demonstrate expertise in molecular methods applied to cyanobacteria and cyanotoxin production, and have experience working with cyanobacteria samples from benthic habitats in California. The contract is solicited under number 140G0325Q0194 by the Department of the Interior US Geological Survey Office of Acquisitions and Grants, with performance to be completed at the contractor's laboratory location.
View the file
Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Sol_140G0325Q0194_Amd_0001.pdf | ||
| Sol_140G0325Q0194.pdf | ||
| 140G0325Q0194.pdf |
On GovTribe
Work with this file on GovTribe
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
STATEMENT OF WORK
I. GENERAL INFORMATION
| A. | Introduction |
| The U.S. Geological Survey (USGS) California Water Science Center (CAWSC) has a requirement for various analyses of DNA from 100 samples. |
| B. | Background |
| The CAWSC is conducting a study in the San Francisco Bay Delta and surrounding areas. Five monitoring stations are visited bi-monthly May–October to collect samples. Samples will be collected starting from August 2025 until October 31, 2026. Samples will be filtered, and the contractor will extract DNA from filters and conduct qPCR analyses of the genes: mcyE, cpcB, anaC, and sxtA. |
C. Definitions/Applicable Documents: N/A
| D. | Scope. |
| DNA preparation and qPCR. |
II. WORK REQUIREMENTS
A. Technical Requirements.
· Chains-of-custody for all laboratory operations, and bioinformatic analyses for each sample, will be fully documented.
· Samples can be received by the Contractor Monday through Friday.
· Extract DNA from filter or biomass samples.
· Perform qPCR for 3 cyanotoxin biosynthesis genes: microcystin (mcyE), anatoxin (anaC), saxitoxin (sxtA).
· AnaC will use the primers published in: Genzoli, L., Hall, R. O., Otten, T. G., Johnson, G. S., Blaszczak, J. R., and Kann, J. (2024). Benthic cyanobacterial proliferations drive anatoxin production throughout the Klamath River watershed, California, USA. Freshwater Science 43, 307–324. doi: 10.1086/731975
· Perform qPCR for the phycocyanin gene: cpcB
· Archive DNA extracts for future DNA sequencing needs
B. Deliverables.
1. Characteristics for work product described in II.A.1., identified by name and quantity.
· Data will be delivered as XLS, CSV, or TXT files. Accompanying summary PDF or PPT files can also be shared alongside the data files.
2. Delivery dates for work/product to be completed and point of delivery.
December 31, 2026
III. SUPPORTING INFORMATION
A. Place of Performance: Contract laboratory location
B. Period of Performance (expressed as number of days, must include the time necessary to test, review, and/or install the deliverable): Award date to December 31, 2026
C. Government Furnished Property (GFP): None
D. Special Considerations.
· The laboratory leader must have a Ph.D. in a field related to cyanobacterial ecology.
· The laboratory must have expertise in molecular methods applied to cyanobacteria and cyanotoxin production.
· The laboratory must have experience working with cyanobacteria and samples coming benthic habitats in California.
File details come from the government source that posted it. Updated .