Sol_140G0325Q0194_Amd_0001.pdf

(x)

140G0325Q0194 x x

1 copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted ; or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGEMENT TO BE

RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR

OFFER. If by virtue of this amendment you desire to change an offer already submitted , such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

x

GSB

SACRAMENTO CA 95819-6027

3020 STATE UNIVERSITY DRIVE EAST

MODOC HALL, CSUS

USGS OAG SACRAMENTO ACQUISITION BR.

07/29/20250001

13. THIS ITEM ONLY APPLIES TO MODIFICATION OF CONTRACTS/ORDERS. IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

12. ACCOUNTING AND APPROPRIATION DATA (If required) is not extended.is extended, Items 8 and 15, and returning

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended , by one of the following methods: (a) By completing

The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offers

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

FACILITY CODE CODE

10B. DATED (SEE ITEM 13)

10A. MODIFICATION OF CONTRACT/ORDER NO.

9B. DATED (SEE ITEM 11)

9A. AMENDMENT OF SOLICITATION NO.

CODE

8. NAME AND ADDRESS OF CONTRACTOR (No., street, county, State and ZIP Code)

7. ADMINISTERED BY (If other than Item 6)CODE 6. ISSUED BY

PAGE OF PAGES

4. REQUISITION/PURCHASE REQ. NO.3. EFFECTIVE DATE2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO. (If applicable)

1. CONTRACT ID CODE

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

07/18/2025

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority) appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

E. IMPORTANT: Contractor is not is required to sign this document and return __________________ copies to the issuing office.

ORDER NO. IN ITEM 10A.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

The purpose of this modification is to extend the response period and update the SOW section of the RFQ for this requirement.

The response period is hereby extended to 08/07/2025, 5:00am PDT.

The updated SOW is as follows and attached:

STATEMENT OF WORK

I. GENERAL INFORMATION

A. Introduction

Continued ...

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)15A. NAME AND TITLE OF SIGNER (Type or print)

15C. DATE SIGNED 16B. UNITED STATES OF AMERICA 15B. CONTRACTOR/OFFEROR 16C. DATE SIGNED

(Signature of person authorized to sign) (Signature of Contracting Officer)

Gerald Lewandowski

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

Previous edition unusable

Except as provided herein, all terms and conditions of the document referenced in Item 9 A or 10A, as heretofore changed, remains unchanged and in full force and effect .

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

2 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140G0325Q0194/0001

The U.S. Geological Survey (USGS) California Water Science Center (CAWSC) has a requirement for the various analyses of DNA from 100 samples.

B. Background

The CAWSC is conducting a study in the San Francisco Bay Delta and surrounding areas. Five monitoring stations are visited bi-monthly

May¿October to collect samples. Samples will be collected starting from August 2025 until October

31, 2026. Samples will be filtered, and the contractor will extract DNA from filters and conduct qPCR analyses of the genes: mcyE, cpcB, anaC, and sxtA.

C. Definitions/Applicable Documents: N/A

D. Scope.

DNA preparation and qPCR

II. WORK REQUIREMENTS

A. Technical Requirements.

Chains-of-custody for all laboratory operations, and bioinformatic analyses for each sample, will be fully documented.

Samples can be received by the Contractor Monday through Friday.

Extract DNA from filter or biomass samples.

Perform qPCR for 3 cyanotoxin biosynthesis genes: microcystin (mcyE), anatoxin (anaC), saxitoxin (sxtA).

AnaC will use the primers published in: Genzoli, L., Hall, R. O., Otten, T. G., Johnson, G. S., Blaszczak, J. R., and Kann, J. (2024). Benthic cyanobacterial proliferations drive anatoxin production throughout the Klamath River watershed, California, USA. Freshwater Science

43, 307¿324. doi: 10.1086/731975

Perform qPCR for the phycocyanin gene: cpcB

Archive DNA extracts for future DNA sequencing needs

B. Deliverables.

1. Characteristics for work product described in II.A.1., identified by name and quantity.

Data will be delivered as XLS, CSV, or TXT files. Accompanying summary PDF or PPT files can

Continued ...

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110

ITEM NO. SUPPLIES/SERVICES QUANTITY UNIT UNIT PRICE AMOUNT

NAME OF OFFEROR OR CONTRACTOR

3 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGE OF

(A) (B) (C) (D) (E) (F)

140G0325Q0194/0001 also be shared alongside the data files.

2. Delivery dates for work/product to be completed and point of delivery.

December 31, 2026

III. SUPPORTING INFORMATION

A. Place of Performance: Contract laboratory location

B. Period of Performance (expressed as number of days, must include the time necessary to test, review, and/or install the deliverable): Award date to December 31, 2026

C. Government Furnished Property (GFP): None

D. Special Considerations.

The laboratory leader must have a Ph.D. in a field related to cyanobacterial ecology.

The laboratory must have expertise in molecular methods applied to cyanobacteria and cyanotoxin production.

The laboratory must have experience working with cyanobacteria and samples coming benthic habitats in California.

Period of Performance: 08/29/2025 to 12/31/2026

NSN 7540-01-152-8067 OPTIONAL FORM 336 (4-86)

Sponsored by GSA

FAR (48 CFR) 53.110