B08_Attachment_B_Pricing_Schedule.xlsx

XLSX spreadsheet 40 KB Posted

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AMPLICON SEQUENCING SERVICES
Solicitation number
140G0226Q0047
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About this file

This is a pricing schedule attachment (SF-18 Continuation Sheet) for genomic sequencing services under a federal contract with the U.S. Geological Survey. The document outlines unit prices and quantities for a base period and five one-year continuation periods, plus a six-month extension option, spanning from August 18, 2025 to August 17, 2031.

The pricing schedule itemizes specialized laboratory and sequencing services including library preparation for 16S V4 amplicon sequencing (1,150 units), library pooling services (12 units), quality control procedures for amplicon library pools (12 and 5 units respectively), and next-generation sequencing runs using both AVITI and Illumina MiSeq platforms with various flow cell configurations. The document also includes pricing for specialty gases required for laboratory operations, such as breathing-grade air, zero-grade air with specified purity standards, research-grade CO2, ultra-high purity helium, and ultra-high purity hydrogen, with quantities ranging from 1 to 840 cylinders depending on the gas type. All unit prices and amounts are currently listed as $0.00, indicating this is a template pending price submissions from contractors. The contract structure allows for price adjustments across each annual period and the extension term.

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Base Period

ATTACHMENT B

SF -18 Continuation Sheet for Pricing
Year 1Year 1Year 2Year 2Year 3Year 3Year 4Year 4Year 5Year 56 mo extension6 mo extension
Item NOSupplies/ServicesQuantityUnitUnit PriceAmountUnit PriceAmountUnit PriceAmountUnit PriceAmountUnit PriceAmountUnit PriceAmount
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
Library Prep for 16S V4 Amplicon Sequencing1,150each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
Library Pooling up to 49-96 samples by qPCR12each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
QC of amplicon library pool prepared by genomics core12each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
QC of amplicon library pool prepared by user (USGS)5each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
AVITI: 2 X 300 bp Medium Capacity, Flow Cell6each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
AVITI: 2 X 300 bp High Capacity, Flow Cell2each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
Illumina: Pre Sequencing QC3each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
Illumina: MiSeq PE250 Flow Cell3each$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0
$0.00$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0$0.00$ - 0

24 EA $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 10 $240.00

840CYL$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
DW$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
MO$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
MO$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
MO$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
MO$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
MO$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00

EA $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00

EA$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
EA$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
EA$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
EA$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
EA$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00
EA$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00$0.00

CYL $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00

1 CYL $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00 $0.00

6 month extension

ATTACHMENT 1
SF -18 Continuation Sheet for Pricing
19 Item NO20 Supplies/ServicesSpecified Equal Cubic Footage21 Quantity22 Unit23 Unit Price24 Amount
BASIC PERIOD- 08/18/2025 -08/17/2026
4007Air Breathing Grade, 260 CF26020CYL$7.18$143.60
4008Air Zero Grade, 1 ppm total hydrocarbons maximum CO 1 ppm max: CO2 400 ppm max: NO 0.1 ppm max, 310 CF31040CYL$59.84$2,393.60
4030CO2 Research Grade 99.999% pure 35 lb35 lb1CYL$239.33$239.33
4031Helium Industrial Grade, 108 CF1081CYL$46.80$46.80
4036Helium Ultra-High Purity, 99.999% 291 CF29160CYL$140.40$8,424.00
4037Hydrogen Ultra-High Purity, 99.999% , <3ppm moisture max, 215 CF21540CYL$294.11$11,764.40
4045Nitrogen Industrial, 304 CF30420CYL$11.97$239.40
4050Nitrogen Ultra-High purity, 99.999% min., 304 CF30420CYL$27.52$550.40
4058Oxygen Zero Grade, 300 CF30060CYL$107.70$6,462.00
4001Acetylene, 99.6 % (grade A) Certified for atomic absorption spectrophotometers, 130 CF1300CYL$150.00$0.00
4002Acetylene, 99.6 % (grade A) Certified for atomic absorption spectrophotometers, 290 CF2900CYL$200.00$0.00
4003Acetylene, MC, Grade A100CYL$10.59$0.00
4004Acetylene, Grade A, 75 CF (lease cylinder must have an exchange specified equal)750CYL$21.54$0.00
4005Acetylene, Grade A, 150 CF1500CYL$33.50$0.00
4006Acetylene, Grade A, 300 CF3000CYL$43.07$0.00
4009Air Zero Grade, 1 ppm total hydrocarbons maximum CO 1 ppm max: CO2 400 ppm max: NO 0.1 ppm max, 275 CF2750CYL$53.85$0.00
4010Air Zero Grade, 275 CF2750CYL$53.90$0.00
4011USP Compressed Air, Medical, 233 CF2330CYL$11.96$0.00
4012Anerobic mixture, 5% Carbon Dioxide, 10% Hydrogen in Nitrogen 10 ppm, 218 CF2180CYL$83.76$0.00
4013Argon, Industrial Grade, 336 CF3360CYL$23.93$0.00
4014Argon, Grade 5 Min Purity 99.999% 02< ppm: THC < 0.2: H2O < 1 ppm, 336 CF3360CYL$89.73$0.00
4015Argon, Grade 5 Min Purity 99.999% 02<1 ppm: THC < 0.2: H2O < 1 ppm, 297 CF2970CYL$71.79$0.00
4016Argon, Grade 5 Min Purity 99.999% 02< 1ppm: THC < 0.2: H2O < 1 ppm, 5275 CFHCF0DW$5.53$0.00
4017Argon, Industrial Grade 249 CF Size #22490CYL$20.34$0.00
4018Argon-Carbon Dioxide c.o. Mix 92 CF Size #5920CYL$0.00$0.00
4019Argon- Hydrogen, 249 CF2490CYL$0.00$0.00
4020Argon, Orca Bulk HCF UnitHCF0HCF$5.53$0.00
402175% Argon, 25% CO2, 125 CF1250CYL$117.11$0.00
4022c.o. Argon-Carbon Dioxide, 92 CF920CYL$0.00$0.00
4023Argon Bulk, Cryogenic Min Purity 99.999%HCF0HCF$5.53$0.00
4024Carbon Dioxide Industrisl Grade gaseous with siphon tube, 50lb50 lb0CYL$8.97$0.00
4025Carbon Dioxide Industrisl , 25lb25 lb0CYL$6.27$0.00
4026Carbon Dioxide Industrisl , 50lb50 lb0CYL$8.97$0.00
4027Carbon Dioxide Industrisl , 100lb100 lb0CYL$14.36$0.00
4028Carbon Dioxide anaerobic grade, 50lb50 lb0CYL$152.94$0.00
4029USP Carbon Dioxide Medical Siphon, 50lb50 lb0CYL$17.95$0.00
4032Helium c.o. Industrial Grade, 38 CF380CYL$15.21$0.00
4033Helium Industrial Grade, 219 CF2190CYL$140.40$0.00
4034Helium Ultra-High Purity, 99.999%, 80 CF800CYL$128.70$0.00
4035Helium Ultra-High Purity, 99.999%, 258 CF2580CYL$140.40$0.00
4038Hydrogen 4.5 Pre-purified Size 2002000CYL$35.90$0.00
4039Methane Grade 3.7 UHP Size 1A2900CYL$288.59$0.00
4040Nitrogen Industrial, 115 CF1150CYL$4.19$0.00
4041Nitrogen Industrial, 20 CF200CYL$4.79$0.00
4042Nitrogen Industrial, 40 CF400CYL$5.07$0.00
4043Nitrogen Industrial, 60 CF600CYL$5.98$0.00
4044Nitrogen Industrial, 230 CF2300CYL$8.97$0.00
4046NF Nitrogen Medical, USP 304 CF3040CYL$15.56$0.00
4047NF Nitrogen Medical, USP 230 CF2300CYL$11.97$0.00
4048Nitrogen Ultra-High purity, 99.999% min., 263 CF2630CYL$23.93$0.00
4049Nitrogen Ultra-High purity, 99.999% min., 263 CF2630CYL$23.93$0.00
4051Nitrogen, Orca Bulk HCFHCF0HCF$0.80$0.00
4052Nitrogen, Bulk cryogenic UHP tank delivery, 100 HCFHCF0HCF$0.80$0.00
4053Nitrogen, LiquidHCF0HCF$0.80$0.00
4054USP Nitrous Oxide Medical, 56 lbs56 lb0CYL$90.00$0.00
4055Oxygen Ultra-High Purity, 99.993% min. H2O <2ppm, 256 CF2560CYL$107.70$0.00
4056Oxygen Ultra-High Purity, 99.993% min. H2O <2ppm, 337 CF3370CYL$137.61$0.00
4057Oxygen Zero Grade 0.5 ppm hydrocarbons max 3ppm moisture max, 99.6% purity, 256 CF2560CYL$95.73$0.00
4059Oxygen avaitor's breathing ABO, 251 CF2510CYL$17.95$0.00
4060Oxygen avaitor's breathing ABO, 307 CF3070CYL$20.94$0.00
4061Oxygen Industrial Grade, 251 CF2510CYL$6.58$0.00
4062Oxygen Industrial Grade, 125 CF1250CYL$4.19$0.00
4063Oxygen Research Grade, 256 CF2560CYL$299.16$0.00
4064USP oxygen, Oxide Medical, 251 CF2510CYL$8.98$0.00
4065c.o. Oxygen Industrial Grade, 92 CF920CYL$5.98$0.00
4066c.o. USP Oxygen Industrial Grade, 25 CF250CYL$6.11$0.00
4067P-5 mixture (5% methane, 95% argon), 227 CF2270CYL$77.78$0.00
4068P-5 mixture (5% methane, 95% argon), 322 CF3220CYL$89.75$0.00
4069P-10 mixture (10% methane, 90% argon), 125 CF1250CYL$71.80$0.00
4070P-10 mixture (10% methane, 90% argon), 227 CF2270CYL$77.78$0.00
4071P-10 mixture (10% methane, 90% argon), 322 CF3220CYL$89.74$0.00
4072Propane, Liquid Forklift 33lb33 lb0CYL$28.91$0.00
4073Propane, Liquid Bulk (1-4lb)GL0GL$3.71$0.00
40740.1%-50% CO2, Bal N2-C(G) size, 249 CF2490CYL$0.00$0.00
40750.3%-30% CO2, Bal Helium-C (G) size, 249 CF2490CYL$0.00$0.00
4076Specialty Gas Mixture 95% argon, 5% hydrogen, 306 CF3060CYL$59.83$0.00
4024ACarbon Dioxide Industrial Grade gaseous with siphon tube, Size #1,75lb75lb0CYL$32.69$0.00
Compliance and Transportation
4077Compliance and Transportation Charge per delivery order10EA$10.00$100.00
Rental
Rental prices shall be those charged for cylinders in the possession of the USGS as of the close of business
on the last Saturday of each calendar month
4078Hi-Pressure cylinder400CYL$3.04$1,216.67
4079Liquid Dewars, orcaDW$23.42$0.00
4080Bulk 1,500 gallon liquid argon Bldg 95MO$295.35$0.00
4081Bulk 3,200 gallon liquid nitrogen Bldg 95MO$295.35$0.00
4082Bulk 1,600 gallon liquid nitrogen Bldg 21MO$0.00$0.00
4083Bulk 1,500 gallon liquid argon Bldg 20MO$295.35$0.00
4084Telemetry of (4) bulk tanksMO$58.40$0.00
*Represening telemetry for (3) bulk tanks
4086Bulk tank installation and equipmentEA$0.00$0.00
Lost or Damaged Cylinder Fee Use Fee
For each cylinder lost or damaged beyond repair, while in the Government's possession, the Government shall
pay to the Contractor the replacement value as follows, less the all allocable rental paid therefore:
4087Lost of Use Fee - only applied if cylinder is lost
Specialty Gas CylinderEA$400.00$0.00
High Pressure CylinderEA$300.00$0.00
Low Pressure CylinderEA$200.00$0.00
Liquid Dewar - 196EA$3,000.00$0.00
Liquid Dewar - 230EA$3,500.00$0.00
Small Medical CylinderEA$100.00$0.00
Hydro testing of Government tanks when requiured by industrial standard
4088Hydro testing per cylinderCYL$35.00$0.00
4089Miscellaneous gas (not specifically listed) and supplies related to gas products such as gas cylinder1CYL$3,000.00$3,000.00
regulators, wall brackets etc. To be purchased on an as needed basis in accordance with the terms
and conditions contained herein.

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