Sol_140FS226Q0141_Amd_0001.pdf

Offers must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended, by one of the following methods:

(a) By completing items 8 and 15, and returning or (c) By separate letter or electronic communication which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by letter or electronic communication, provided each letter or electronic communication makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

E. IMPORTANT: is not is required copies to the issuing office.

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT 1. CONTRACT ID CODE

2. AMENDMENT/MODIFICATION NUMBER 3. EFFECTIVE DATE 4. REQUISITION/PURCHASE REQUISITION NUMBER 5. PROJECT NUMBER (If applicable)

7.ADMINISTERED BY (If other than Item 6) CODE

STANDARD FORM 30 (REV. 11/2016)

Prescribed by GSA FAR (48 CFR) 53.243

FACILITY CODE

9A. AMENDMENT OF SOLICITATION NUMBER

9B. DATED (SEE ITEM 11)

10A. MODIFICATION OF CONTRACT/ORDER NUMBER

10B. DATED (SEE ITEM 13)

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

The above numbered solicitation is amended as set forth in Item 14. is extended. is not extended.

12. ACCOUNTING AND APPROPRIATION DATA (If required) copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted;

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NUMBER AS DESCRIBED IN ITEM 14.

CHECK ONE A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE CONTRACT ORDER

NUMBER IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation data, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(b).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15C. DATE SIGNED

15A. NAME AND TITLE OF SIGNER (Type or print)

16C. DATE SIGNED

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

PAGE

6. ISSUED BY CODE

8. NAME AND ADDRESS OF CONTRACTOR (Number, street, county, State and ZIP Code) (X)

CODE

15B. CONTRACTOR/OFFEROR

(Signature of person authorized to sign)

16B. UNITED STATES OF AMERICA

(Signature of Contracting Officer)

Previous edition unusable

PAGESOF

Contractor

The hour and date specified for receipt of Offers to sign this document and return

Dana Arnold

See continuation page

FWS SAT 2

FWS, SAT Team 2 Falls Church VA 22041

FS2

140FS226Q0141

07/14/2026

07/14/2026

1 3

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

140FS226Q0141/0001

IN - INDIANA ES FIELD OFFICE - Bat Genomic

Assessment, 0001

FWS PPR #2117692

The Government is providing the following additional information to assist offerors in preparing quotations.

Sample Storage

The U.S. Fish and Wildlife Service's Indiana

Ecological Services Field Office (INFO) does not have an on-site -80°C freezer. As a result:

-A portion of the MYSO wing punch samples are currently being stored by Dr. Mark Davis at the

University of Illinois.

-Additional MYSO samples are being stored by the Indiana Department of Natural Resources in

Bloomington, Indiana.

Previously Processed Samples

-Over the past year, the Davis Laboratory (Dr.

Mark Davis and Jordyn Chase) processed a subset of the MYSO samples, at no cost to the

Government, while utilizing available capacity during sequencing for a separate research project.

- The Davis Laboratory understands that these data will be shared with the contractor ultimately awarded this requirement.

The Government is currently coordinating with the Davis Laboratory to determine:

-The exact number of samples that have already been processed; and

-Which of those samples will be included in the final genomic analysis under this requirement.

**This information will be provided once finalized.

Compatibility Requirements

Because a portion of the samples has already been processed, offerors must ensure that their proposed sequencing approach is compatible with the existing dataset.

Offerors may propose using the same library preparation and sequencing methodology employed

Continued...

CONTINUATION SHEET

REFERENCE NO. OF DOCUMENT BEING CONTINUED PAGES

NAME OF OFFEROR OR CONTRACTOR

SUPPLIES/SERVICES

(B)

UNIT

(D)

UNIT PRICE

(E)

AMOUNT

(F)

OPTIONAL FORM 336 (4-86)

Sponsored by GSA FAR (48 CFR) 53.110

ITEM NO.

(A)

QUANTITY

(C)

NSN 7540-01-152-8067

140FS226Q0141/0001

by the Davis Laboratory (ddRADseq), or they may propose an alternative approach. Regardless of the methodology proposed, the quotation must satisfy all requirements identified in the

Statement of Work, including the required sequencing depth and data quality (e.g., sequencing approximately 200-400 individuals at greater than 15-20x coverage per individual).

Low-coverage sequencing will not be accepted.

Quotations should clearly identify all costs associated with meeting these data quality requirements.

The previously processed samples were prepared using the following methodology:

-Library preparation: ddRADseq using the Sacks protocol

-Restriction enzymes: MspI and PstI

-Size selection: 370-720 bp (final library size with adapters; approximately 370-680 bp prior to adapter addition)

-Sequencing platform: Illumina NovaSeq 6000

-Read configuration: 150 bp paired-end reads

-Additional details regarding the library preparation protocol can be found in the referenced dissertation (page 90 of the dissertation; page 96 of the PDF including front matter):

https://www.ideals.illinois.edu/items/126823

Please note:

- If proposing additional ddRADseq work, the library preparation protocol must be compatible with the methodology described above so that the datasets can be combined.

- Whole Genome Sequencing (WGS) may also be proposed; however, the resulting data must be compatible with the existing ddRAD dataset, including mapping to the same reference genome.

Period of Performance: 10/01/2026 to

12/31/2027

Sponsored by GSA FAR (48 CFR) 53.110