Statement of Work.pdf
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- Solicitation number
- 12905B24Q0023
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This request for quotation solicits proximity ligation library preparation and sequencing services for three plant species. The Agricultural Research Service seeks a contractor to prepare Hi-C libraries for tall fescue, hard fescue, and bush penstemon, with genome sizes of 9Gb, 7Gb, and 800Mb respectively, requiring four, three, and one library preps. Low coverage sequencing is required for quality control, and one NovaSeq lane of sequencing for all three species combined. Leaf tissue samples will be provided from Logan, Utah. Offers are due by January 20th, 2024 and the contract is set aside for small businesses. The selected contractor must complete the services within 90 days of award at the delivery address provided in Logan, Utah.
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| File | Type | Posted |
|---|---|---|
| Terms and Conditions (2).docx | DOCX document |
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ARS Forage and Range Research Laboratory request proximity ligation library preparation and Illumina-based sequencing for three species: tall fescue, hard fescue, and bush penstemon. Tall and hard fescues have 9Gb and 7Gb genome sizes, respectively, and would thus need 4 and 3 library preps. Bush penstemon has a 800 Mb genome size and would only need 1 library prep. Then, all three species will need sufficient sequencing coverage for genome scaffolding from the proximity ligation.
Fresh leaf tissue will be sent to the successful contractor on dry ice from the Logan location.
The technical acceptability is based on (1) the ability to prepare Hi-C libraries for each of the three species, (2) the ability to conduct low coverage sequencing for library QC at less than 1M reads per species for each of the three species, and (3) the ability to sequence one S4 NovaSeq lane (or equivalent numbers of sequence reads) for all three species combined.
Services to be completed 90 days after award Delivery Address: 696 North 100 East, Logan UT 84322
Statement of Work
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