RFI No. 01.pdf
PDF 142 KB Posted
- Solicitation number
- 12905B23Q0117
About this file
This document contains a Request for Information (RFI) for RNA sequencing services. The RFI seeks input on deliverable formats, sample preparation requirements, and sequencing specifications for a project to sequence RNA from animal tissue samples for the USDA Agricultural Research Service located in Albany, California. Responses are due by June 28, 2023 and should reference specification or drawing numbers as applicable. The deliverable will include 80 GB of accessible RNA expression data on the contractor's website and later transferred to cloud storage. Samples will not be isolated by the contractor as required for sequencing.
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Other files
| File | Type | Posted |
|---|---|---|
| Sol_12905B23Q0117_Amd_0002.pdf | ||
| RFI Form 2 QandA.docx | DOCX document | |
| STATEMENT OF WORK.pdf | ||
| RFI Form.pdf | ||
| TandCs with PandCs for 12905B23Q0117.docx | DOCX document |
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Text version
REQUEST FOR INFORMATION (RFI)
PROJECT NUMBER: Please ensure that before submitting questions or requests for clarification that you thoroughly read the solicitation, specifications, drawings and other pertinent documents. When submitting questions on this project the Government requires contractors to specifically identify the specification and/or solicitation section(s) or drawing number(s) in reference to the question or request for clarification submitted. No question or request for clarification will be answered by the Government unless the above requirements are met. Failure to comply may prevent the Government from responding in a timely manner.
PROJECT TITLE:
PROJECT LOCATION:
SEND TO:
Email:
On subject line of email include the following: “ – RFI#___“
RFI #: DATE: SPEC/DWG. REFERENCE(s):
INFORMATION NEEDED:
REPLY:
Reply will be issued by Solicitation Amendment by the Contracting Officer
Offerors are responsible to retrieve and acknowledge all Amendments
ATTACHMENTS:
SUBMITTED BY: City/State:
PHONE NO.:
EMAIL:
http://www.beta.sam.gov/ Jeffery.Ridenour Highlight
Jeffery.Ridenour Highlight
COR Comments:
| PROJECT NUMBER: 12905B23Q0117 |
| Construction IDIQ: RNA Sequencing of animal tissues |
| U S Department of Agriculture Agricultural Research Service Pacific West Area 800 Buchanan Street Albany CA 94710: USDA, ARS, HPFRU |
800 Buchanan Avenue Albany, CA 94710
| SUBMITTED BY: |
| CityState: |
| PHONE NO: |
| EMAIL: |
| RFI: 01 |
| DATE: 6/28/2023 |
| SPECDWG REFERENCEs: N/A |
| INFORMATION NEEDED: (Q)What form should the deliverable take? Email, website post, print? |
(A)The deliverable is 80 Gb of RNA expression data accessible on their website and later transferred to our cloud storage. These are very large files, which cannot be sent via “emails”.
(Q)The lab requires the submission of isolated total RNA or DNA samples. It does not perform RNA or DNA isolations for Illumina sequencing. Do you know if the samples will be isolated?
(A)No, samples’ RNAs will not be isolated.
(Q)RNA samples need to be DNA-free and should be dissolved in molecular biology grade water (RNAse-free; not DEPC treated). DNA samples need to be RNA-free and dissolved in EB buffer or TLE buffer. Would the samples meet these requirements?
(A)No. Since samples’ RNAs will not be isolated.
| ATTACHMENTS: |
| Text1: 12905B23Q0117 |
| Text2: USDA/ARS/PWA–Shelley Steen |
| Text3: shelley.steen@usda.gov |
| Text4: |
| Text5: |
| Text6: |
File details come from the government source that posted it. Updated .