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This document contains questions and answers regarding a Request for Information (RFI) for RNA sequencing of mouse tissue samples. The RFI is seeking information on sample preparation and format, with the samples consisting of approximately 100mg of frozen muscle tissue from C57Bl/6J mice obtained from Jackson Labs. Responses to the RFI will be provided via a solicitation amendment issued by the contracting officer. The related federal contract opportunity is a solicitation from the USDA Agricultural Research Service to provide library preparation, quantification, and analysis of animal tissue samples for RNA sequencing work at their Healthy Processed Foods Research Unit in Albany, California.

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RFI No. 01.pdf PDF
Sol_12905B23Q0117_Amd_0002.pdf PDF
STATEMENT OF WORK.pdf PDF
RFI Form.pdf PDF
TandCs with PandCs for 12905B23Q0117.docx DOCX document

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REQUEST FOR INFORMATION (RFI)

PROJECT NUMBER:
12905B23O0117
...
Please ensure that before submitting questions or requests for clarification that you thoroughly read the
PROJECT TITLE:
RNA Sequencing of animal tissues I solicitation, specifications, drawings and other pertinent
PROJECT LOCATION:
USDA ARS HPFRU

800 B chan n Avenue Albany CA 94710 documents. When submitting questions on this project the Government requires contractors to specifically identify the specification and/or solicitation section(s) or drawing number(s) in reference to the question or request for clarification submitted. No question or request for clarification will be answered by the Government unless the above requirements are met. Failure to comply may prevent the Government from responding in a timely manner.

SUBMITTED BY:

City/State:

SEND TO: USDA/ARS/PWA-Shelley Steen

Email: shelley.steen@usda.qov On subject line of email include the following:" 1290582300117

RFI#:
DATE:

7/3/2023 SPEC/DWG. REFERENCE(s):

INFORMATION NEEDED:

Are the samples going to be provided as frozen whole samples, frozen sliced sample, pre-ground frozen powdered samples, or pre-lysed in a buffer like Trizol or Qiazol? Or are they preserved in some sort of preservative like RNA later, etc.?

1. Samples are frozen without preservative.

If they are whole tissue or slices, what are the approximate size and weight (e.g. 2mm x 2mm x 2mm, 10 grams)

1. Approximate size: 100 mg

What animal is the tissue being collected from? Is the sample transgenic or wild type? Is the animal raised in the lab or caught in the wild? Where is the animal from ( e.g. in California, another state, another country)?

1. Species: Mus musculus. C57Bl/6J from Jackson Labs.

REPLY:

Reply will be issued by Solicitation Amendment by the Contracting Officer

Offerors are responsible to retrieve and acknowledge all Amendments

ATTACHMENTS:

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