SOW.pdf

Statement of Work Project: Acquisition of an Automated Cell Counting and Assessing Device

GENERAL INFORMATION

1.0 Scope of Work: Acquisition of an automated device that can count and assess the viability of cells for single cell sequencing for gene expression.

2.0 Background: Acquisition of an automated device that can calculate the size, count, concentration, and viability of cells used generate single cell partitions cells for transcriptomic and epigenomic research. Must be able to count and assess the viability of hundreds of thousands of cells 5-80 microns in size.

CONTRACTOR REQUIREMENTS

3.0 Technical Requirements/Tasks:

The automated cell counting device must meet the following minimum specifications:

o Must be capable of counting and assessing the viability of hundreds of thousands of cells o Must be capable of calculating the size, count, concentration, and viability of cells in less than 60 seconds o Must be compatible with sample types including cultured cell lines, PBMCs, primary cells, and whole blood o Must utilize both dual fluorescence and brightfield imaging in processing o Must have cell size flexibility for cells 5-80 microns in size o Must be capable of counting a minimum of 8 samples at a time o Must be capable of processing volumes as small as 10ul o Must allow user to customize experiment settings and save commonly used assays o Must have both slide and image-based autofocus methods o Must have bi-directional stage movement to capture maximum sample volume o Must detect sample slides automatically o Must begin counting as soon as the slide is inserted o Must illuminate samples with high power LEDs o Must include a glove-friendly touchscreen o Must include an on-screen keyboard for custom naming of files o Must be able to perform multiple replicates within a single slide o Must be compatible with disposable slides for infectious samples o Must be compatible with slides composed of shatterproof plastic materials o Must be compatible with slides that have mitigated cross-contamination risk o Must include supply and power cord o Must have at least 2 ports on the front and back of the instrument o This unit should be plug-in and run, requiring minimal installation other than to level and check functionality o System shall be benchtop style instrument with dimensions not to exceed 10” wide x 14” deep and no more than 19” tall.

o Qty: 1 system.

4.0 Deliverables/Schedule This unit should be delivered onsite within 60 days of date of award.

5.0 Evaluation Factors Technical capability, deliverability and price with technical capability and deliverability more important than price.

6.0 Technical Point of Contact: Should there be technical questions, please contact

Kaitlyn Sarlo Davila at 515-337-6772.

7.0 Place of Delivery: USDA, 1920 Dayton Avenue, Bldg 24, Ames, IA 50010