Statement of Work-170 Cotton DNA sequencing-revised.docx
DOCX document 20 KB Posted
- Attached to
- Sequencing 170 Cotton DNA Federal contract opportunity
- Solicitation number
- 12405B24Q0117
About this file
This document is a Statement of Work for a federal contract opportunity to sequence 170 cotton DNA samples. The USDA is seeking a vendor to perform the following tasks: measure the DNA quantity and quality, construct specialized DNA libraries using a PCR-free method, sequence the libraries using an Illumina or similar next-generation sequencing instrument, and provide bioinformatics analysis to generate SAM, BAM, and VCF files by aligning the sequence data to a reference genome. For 168 samples, the vendor will generate 24Gb of sequence data per sample, and for 2 samples, the vendor will generate 100Gb of sequence data per sample. The vendor must complete the work within 45 business days of receiving the DNA samples. The associated federal contract opportunity, Solicitation Number 12405B24Q0117, is a request for quote (RFQ) seeking the sequencing service. The contract will be awarded on a Firm Fixed Price basis to the vendor that provides the best value based on technical specifications and price.
View the file
Other files for this federal contract opportunity
| File | Type | Posted |
|---|---|---|
| Beta Sam Solicitation.docx | DOCX document | |
| DF - Statement of Work-170 Cotton DNA sequencing.docx | DOCX document | |
| Clauses and Provisions 12405B24Q0117.docx | DOCX document | |
| Beta Sam Solicitation.docx | DOCX document |
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Statement of Work
Sequencing 170 cotton DNA samples:
The USDA intends to purchase a service to sequence 170 cotton DNA samples. Upon receiving DNA samples from the customer, the vendor will first measure the DNA quantity, and check the quality to determine whether the DNA sample is good for library construction. After passing the quality control, the vendor will use a PCR-free preparation method to construct a specialized library using the provided DNA sample. Then the libraries will be sequenced using a next-generation sequencing instrument such as Illumina 2000, NovaSeq or similar. A pair end 150 is required. For 168 samples, the vendor will generate 24Gb sequence data from each sample. For the remaining 2 samples, the vendor will generate 100Gb sequence data from each sample. We would like the vendor to provide basic bioinformatics analysis that includes generation of SAM, BAM, and VCF files by aligning to JGI reference Upland cotton (Gossypium hirsutum cv. TM-1) genome. The vendor will upload all sequence data into a hard disk, and ship to the customer.
The vendor will complete the work within 45 business days after receiving DNA samples from the customer.
File details come from the government source that posted it. Updated .