DF - Statement of Work-170 Cotton DNA sequencing.docx

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Sequencing 170 Cotton DNA Federal contract opportunity
Solicitation number
12405B24Q0117
Issued by
Department of Agriculture Agricultural Research Service Field Research Implementation and Information Delivery

About this file

This combined synopsis and solicitation requests quotes for sequencing 170 cotton DNA samples. The USDA Agricultural Research Service seeks a vendor to measure DNA quantities, check quality, construct specialized libraries from the samples, sequence the libraries using next-generation sequencing with paired end 150 required, and upload 24GB of data from 168 samples and 100GB from the remaining 2 samples to a hard drive for delivery. The work must be completed within 45 business days of receipt of samples. Quotes are due by March 22, 2024 and must include technical specifications and pricing, with award based on best value.

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File Type Posted
Beta Sam Solicitation.docx DOCX document
Statement of Work-170 Cotton DNA sequencing-revised.docx DOCX document
Clauses and Provisions 12405B24Q0117.docx DOCX document
Beta Sam Solicitation.docx DOCX document

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Statement of Work

Sequencing 170 cotton DNA samples:

The USDA intends to purchase a service to sequence 170 cotton DNA samples. Upon receiving DNA samples from the customer, the vendor will first measure the DNA quantity, and check the quality to determine whether the DNA sample is good for library construction. After passing the quality control, the vendor will use a PCR-free preparation method to construct a specialized library using the provided DNA sample. Then the libraries will be sequenced using a next-generation sequencing instrument such as Illumina 2000 or NovaSeq. A pair end 150 is required. For 168 samples, the vendor will generate 24Gb sequence data from each sample. For the remaining 2 samples, the vendor will generate 100Gb sequence data from each sample. The vendor will upload all sequence data into a hard disk, and ship to the customer.

The vendor will complete the work within 45 business days after receiving DNA samples from the customer.

File details come from the government source that posted it. Updated .