Q and A Enzymatic Methyl.pdf
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- Solicitation number
- 12305B24Q0202
About this file
This document is a set of questions and answers regarding the NEBNext Enzymatic Methyl-seq kit for a federal contract opportunity. The key details are:
The contract opportunity is for Enzymatic Methyl-Seq services, including DNA extraction/QC, library preparation, and sequencing, for 106 samples from liver, muscle, and mammary gland/skin tissue. The USDA Agricultural Research Service (ARS) is seeking these services under a Firm Fixed-Price contract to be awarded on a Lowest Price Technically Acceptable basis. Proposals are due by September 5, 2024. The work will be performed at the USDA ARS facility in Beltsville, MD. The solicitation is a small business set-aside under NAICS code 541380.
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Other files
| File | Type | Posted |
|---|---|---|
| SOW_EMSeq.pdf | ||
| CLAUSES_PROVISIONS.pdf | ||
| WD.pdf |
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Enzymatic Methyl-Seq Q&A
1. NEBNext Enzymatic Methyl-seq Kit: The NEBNext Enzymatic Methyl-seq kit alone should suffice for our needs, so we do not require the additional module. However, you may need DNA isolation or other QC kits.
2. Sample Batch: We plan to send 106 samples in 2-3 batches. Additional batches may be needed to account for any failed samples.
3. Starting Material: The starting material will be liver, muscle, and mammary gland/skin, and it will be sent in small Whirl-Pak bags or 1.5 mL microcentrifuge tubes.
4. Amount for Extraction: We will provide a few grams of material per sample for extraction, and we are not limited by the amount of tissue available.
5. Sample Quality and Type: The samples will be freshly collected from local abattoirs within 1 hour of slaughter and snap-frozen on dry ice.
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