AMEND 0001 Attachment 3 - Questions and Answers.pdf
PDF 156 KB Posted
- Attached to
- Processing Experimental Samples
- Solicitation number
- 12305B24Q0072
About this file
This document is a set of Questions and Answers related to a federal contract opportunity for Processing Experimental Samples, solicitation number 12305B24Q0072.
The key details are:
- This is a brand new contract, with no incumbent, for whole-genome shotgun sequencing services for 137 intestinal content samples and short chain fatty acid (SCFA) determination in 94 of these samples.
- The samples were collected from mice and flash frozen. The government prefers to keep the protocols as outlined in the Statement of Work.
- The contract is a 100% small business set-aside with a NAICS code of 541990 and a size standard of $19.5M.
- Offers must demonstrate technical capability to meet the Performance Elements and Benchmarks in the Statement of Work, provide a firm fixed price quotation, and include a Unique Entity ID.
- Quotes are due by 4/17/2024 at 2:00 PM EST, with questions due by 4/15/2024.
- The contracting officer for this opportunity is Jennifer Peterson, who can be reached at Jennifer.L.Peterson@usda.gov.
View the file
Other files
| File | Type | Posted |
|---|---|---|
| WD 2015-4281.pdf | ||
| Attachment 2 - Contract Terms and Coniditons.pdf | ||
| Attachment 1 - Statement of Work.pdf |
On GovTribe
Work with this file
- Download the original file
- Contacts named in this file
- Similar government files
- Ask GovTribe AI about this file
Text version
Atachment 3 - Ques�ons and Answers
How were the intes�nal contents collected? I am guessing endoscopy or lavage. Are they stored in a buffer or will it be flash frozen?
Intestinal content samples were collected in cryotubes after sacrifice. Samples were flash frozen with liquid nitrogen before storing them at -80C.
Are the informa�c protocols outlined in the SOW set in stone, or can we alter those if we determine a beter fit?
We prefer to keep as described in SOW. We want to keep consistency in data being generated.
Same ques�on except for the instrumenta�on, we don't have any GC-FIDs but we do have GC-MSs
We want to keep the GC_FIDs as proposed.
Are they human samples?
No these are samples derived from mice.
What species are these samples?
Mice fecal samples
What amount of starting material can you provide?
50-100 mg
What's the biosafety level of your samples? Does the sample include any natural reservoir species, potential pathogens, transgenic organisms, genetically modified organisms, toxins, or prions?
No known pathogens
Have your samples been exposed to any viral vectors, viruses, or pathogens during handling or processing? Please list any that apply, regardless of biosafety level.
No exposure to viruses or other pathogens
When would you like to send samples?
See Statement of Work 5.0 Schedule/Deliverables, all work is to be completed within 4 months after the contract is established.
Who is the lab PI?
At Tufts University HNRCA: Dr. Stefania Lamon-Fava, PhD; at Beltsville USDA: Dr.
Gloria Solano-Aguilar
We would like to know if this is a brand new contract OR if there is (was) an incumbent performing these services:
Brand New Contract, we have done many studies, however this a new requirement no incumbent.
File details come from the government source that posted it. Updated .