1-RFQ01-2504741B01-TRANSL-XXXXXX.pdf

Procurement Services Request for Quote (RFQ) Form

Solicitation Number: RFQ01-2604741B01-TRANS-XXXXXX

FLORIDA VIRTUAL SCHOOL

5422 Carrier Drive, Suite 201, Orlando FL 32819

REQUEST FOR QUOTATION

Procurement Contact:

Email: kstolarenko@flvs.net

Request Date:

October 23, 2025

Questions Due:

October 27, 2025, No Later than 2:00 PM EST

Responses Due:

November 3, 2025, No Later than 2:00 PM EST

RFQ Title: Curriculum Course Translations Services RFQ Number: RFQ01-2604741B01-TRANS-XXXXXX

Services Implementation Products Licensing Other https://flvs.bonfirehub.com/opportunities Technical assistance:Support@GoBonfire.com

OVERVIEW

FLVS is seeking a service provider to provide Curriculum Course Translation Services from English to Spanish. Work efforts shall utilize a combination of existing technology and human resources to satisfy requirements. This project has a not to exceed budget of $50,000. Respondents shall offer their most competitive rates offering FLVS the best value for the deliverables. Any use of open-source AI is strictly prohibited.

I. GENEARAL INFORMATION

1. DUE dates/times related to RFQ events are listed above in Eastern Standard Time and are subject to change with notice on Bonfire.

2. Respondents must complete and submit with their response the Compliance Work Sheet (Attachment 1), any applicable terms and conditions (in the event of a conflict, the FLVS Addendum (Attachment 2) shall take precedent), the Vendor Application, W9 form, FLVS E-Verify Certification, and Foreign Country of Concern Attestation (Attachment 3). In addition, the awarded Contactor will be required to complete a Forced Labor Affidavit and Student Information Form.

3. Contract Term: This RFQ is bound by the terms and conditions outlined in this document, the FL DMS State Contract and the FLVS Addendum. The initial term shall be one (1) year, optional three (3) year renewals.

4. Evaluation of Responses

• All quotes properly completed and submitted by the deadline shall be accepted for consideration by FLVS.

• Any quotes that are incomplete or non-compliant with the instructions and/or submittal terms and conditions may be rejected and excluded from further consideration at FLVS’s sole discretion.

• FLVS shall review all submissions to obtain the best value. Responses to this RFQ will be reviewed for compliance to the Requirements, price, past performance, experience and qualifications, special features that may be offered, maintenance terms and availability. FLVS will analyze each submission to determine if basic criteria have been met, as well as comparing price.

• FLVS reserves the right, before awarding the contract, to require respondent to submit additional evidence of qualifications or any other information FLVS may deem necessary. FLVS reserves the right to request clarifications at any time throughout the evaluation and negotiation process. FLVS may, at its discretion, short list and interview respondents.

• FLVS reserves the right to make award(s) by individual item, group of items, all or none or a combination thereof.

FLVS may, at its sole and absolute discretion, reject any and all, or parts of any and all responses; re-advertise this RFQ; postpone or cancel, at any time, during this RFQ process; or waive any irregularities in this RFQ, or in the responses received as a result of this RFQ. Terms, and Conditions and/or Exceptions submitted by the Respondent which differ from those of the solicitation may be cause for disqualification.

• The selected Contractor has shall be notified.

mailto:kstolarenko@flvs.net https://flvs.bonfirehub.com/opportunities mailto:Support@GoBonfire.com

Solicitation Number: RFQ01-2604741B01-TRANS-XXXXXX

RFQ01-2504741B01-TRANSL-XXXXXX

Curriculum Course Translations

TABLE OF CONTENTS

Section Page

I. Overview and General Information Cover

II. Submission Instructions and Response Documents 3

III. Scope of Services

A. Audit Scope, Objectives & Methodology B. Implementation Plan - Milestones/Timeline

IV. Exceptions and Definitions 8

V. Exhibits 9

VI. Pricing Sheet 14

TABLE OF CONTENTS - ATTACHMENTS

Attachment Number

Title

1 Compliance Worksheet

2 FLVS Master Service Agreement and Statement of Work Template (Samples)

3 Required Forms:

• Vendor Application

• W-9

• E-Verify Certification

• Foreign Country of Concern Attestation

• Non-Disclosure Agreement

II. SUBMISSION INSTRUCTIONS

Responses must be received by FLVS Procurement Department through Bonfire at:

https://flvs.bonfirehub.com/ no later than the response due date and time specified above. Responses shall not be modified after the RFQ closing date and time.

Respondent is responsible for allowing adequate time to upload their documents to https://flvs.bonfirehub.com. If you’re experiencing technical difficulties, contact support@gobonfire.com.

• Upload files in Adobe Portable Document Format (.pdf), Excel (.xls or .xlsx) (No Zip Files shall be accepted) as directed by the Bonfire placeholders provided.

• All required documents must be completed in their entirety and signed by an official who is authorized to legally bind the Respondent on all solicitation specifications

• All documents must reference the LEGAL name of the proposer exactly as it appears under business registration of state where registered/incorporated. The LEGAL name must match the FEIN or Tax ID number provided. Do not submit a proposal under a d/b/a (“doing business as”) or a fictitious business name.

Response Documents In order to maintain comparability and consistency in the review of responses, all responses shall be organized as specified below. Avoid any elaborate promotional materials and provide only information that is required. All supporting materials should clearly reference the portion of the RFQ to which they pertain. Submit the following separate electronic documents in PDF format.

Cover Letter, Background and Qualifications. Respondents shall include:

• A cover letter demonstrating understanding of the requirements/scope of services of this RFQ. Also include contact information for the primary contact for this RFQ and principals and/or authorized signatures.

• Describe any strategic advantages your organization provides if selected for award

• Include any assumptions and/or constraints

• Prior experience with K12 school districts and public/government agencies

• A completed Compliance Work Sheet (Attachment 1) (Required)

• Completed Vendor Application, W-9, and E-Verify Affidavit, and Foreign Country of Concern

Attestation (all Required) – These forms must be completed, signed where indicated without revision.

No exceptions, no incomplete or blank spaces shall be accepted.

• Section III. Scope of Services A. Translation Scope, Objectives & Methodology. The respondent is responsible for including the full scope of services in their proposed pricing (Section IV) and implementation plan. Unless indicated in the Exceptions/Alternatives section, the respondent shall affirmatively confirm that they will meet or exceed the scope.

B. Workflow/Implementation Plan. Respondent shall describe the plan for performing the work identified in the scope of services section.

https://flvs.bonfirehub.com/opportunities/43114 mailto:support@gobonfire.com

• Section IV. Pricing Sheet. The contractor shall provide a price sheet containing a firm fixed price for all services to be provided under the prospective contract.

III. A. SCOPE OF SERVICES

FLVS is seeking providers for course translations; English into other languages, starting with Spanish. FLVS currently has more than 200 curriculum courses created in English. FLVS is looking for the translation of two courses from English to Spanish, with potential for additional translation work. Although, additional course work is not guaranteed. The translated course information shall be of high quality with the Spanish terms making sense in the context of the curriculum. All Curriculum course content, images, interactives, support documents, ancillary documents, assessments, videos and audio should be translated completely and accurately. Respondents shall sign provided forms, including the Non-Disclosure Agreement as part of their response. All work shall be performed in accordance with the terms specified within the agreed upon Statement of Work following issuance of an FLVS Purchase Order.

Provider must offer an existing, fully developed language translation tool or application. FLVS is not seeking development of a new tool for this project. The proposed solution must not utilize Google translate. The solution must not incorporate or rely on any open-source AI platforms, such including but not limited to ChatGPT, Gemini or other similar.

FLVS content, data, and proprietary information is strictly confidential and must not be used, shared, or incorporated into any open-source AI platform or publicly accessible system under any circumstances.

There is zero tolerance for unauthorized exposure, distribution, or use of this material.

The proposed solution shall also include a clear methodology to ensuring translation accuracy and use of appropriate language, and tonality. This shall include resources that provide subject matter expertise, and quality assurance.

DELIVERABLES

Course Modules Algebra 1 v29 Biology 1 v28 Lessons 13 6 Pages 99 57 Interactives 534 783 Videos 62 109 Audio 2 39 Images 977 526 Documents 71 84 Answer Key Documents 12 11 Assessments (Exams and Items) 92 exams

2922 items 50 exams 2271 items

Assessment Images 1794 221

Note: See Attachment for details below.

SPECIFICATIONS/REQUIREMENTS/ASSUMPTIONS

If awarded, Contractor agrees to meet or exceed the following requirements. A response of “Yes” confirms agreement to include within the pricing. Note: Items flagged with “R” are required, items flagged with “H” are highly desired.

No. Description of General Requirements Included Yes or No

1 R The solution includes an existing, fully developed language translation tool or application.

☐ Yes ☐ No

2 R Solution does not utilize open-source AI ☐ Yes ☐ No 3 R Contractor will translate course content pages (JSON/HTML). ☐ Yes ☐ No 4 R Contractor will translate content in interactive files. ☐ Yes ☐ No 5 R Contractor will translate support documents within course content (copyright footer excluded).

☐ Yes ☐ No

6 R Contractor will translate ancillary documents in Answer Key files (copyright footer excluded).

☐ Yes ☐ No

7 R Contractor will reproduce any image files with text translated. ☐ Yes ☐ No 8 R Contractor will translate audio and produce a translated audio track and text version.

☐ Yes ☐ No

9 R Contractor will translate videos and produce a translated audio track and text version.

☐ Yes ☐ No

10 R Contractor will translate assessments. ☐ Yes ☐ No 11 R Contractor will reproduce any image files with text translated within assessments.

☐ Yes ☐ No

12 H Proposal includes a dedicated point of contact for all contract activity.

☐ Yes ☐ No

13 H Proposal includes resources available to perform services during FLVS standard hours of operation (Monday through Friday between 8:30AM and 5:00 PM EST).

☐ Yes ☐ No

14 R Warranty of Deliverables. In the event defects of deliverables are identified within 12 months from the date of acceptance, Contractor shall execute corrections within seven (7) business from notification at no additional cost.

☐ Yes ☐ No

15 R Quality Expectation. It is the expectation that assets created by the Contractor will be of high quality on first review, such that only one round of FLVS curriculum specialist’s review moves the file to approval and completion. This process is included within the proposed pricing. However, if the contractor submits assets that still do not meet the requirements as indicated by the feedback provided, the contractor will continue with the editing process until the items are approved and accepted by FLVS at no cost to FLVS.

☐ Yes ☐ No

16 R Intellectual Property/Data Ownership. All source files, content, and deliverables shall be construed as a work product and shall be the exclusive property of FLVS in perpetuity, free of copyright licensing without restriction from contractor and any subcontractor(s). Upon termination or completion of contract, all FLVS data shall be returned to FLVS within five (5) business days in the format specified.

☐ Yes ☐ No

17 H Contractor agrees to attend status meetings/calls on daily or weekly bases as needed at the discretion of FLVS.

☐ Yes ☐ No

18 R Proof of Concept. Contractor will be required to create a Proof of Concept (POC) of a few lessons (directly following the project kickoff meeting) that will demonstrate their ability to translate lessons meeting the quality and expectations of FLVS (within these requirements). If the POC does not meet the quality expectations required by FLVS, the POC will be rejected and will need to be revised within 24 hours. The POC will require revisions until approved by the FLVS project team. Rejection of the POC lesson shall not impact the overall project timeline. It will be the responsibility of the contractor to make concessions to stay on the established project timeline established by FLVS.

☐ Yes ☐ No

19 R Contractor shall adhere to the agreed upon milestone due dates and project completion date.

☐ Yes ☐ No

20 R Solution includes both technology and human resources to complete the work effort and has been fully defined within the proposed methodology.

☐ Yes ☐ No

21 R Respondent has reviewed the attached FLVS Cybersecurity requirements and agrees to comply with all applicable standards.

This also includes the following as applicable if awarded:

1. For Contractor-hosted solutions adherence with SOC 2 as required by F.S. 501.171

2. Encryption of student PII bot at rest and in transit

3. Adherence with Breach Notification Procedures as defined in F.S. 501.171

4. Adherence with handling of any student data in accordance with F.S. 1002.22 and F.S. 1002.221

Solicitation Number: RFQ01-2604741B01-TRANS-XXXXXX

RESPONDENT QUESTIONNAIRE: Provide complete, detailed responses to each question listed below. Additional space may be used as needed.

RESPONDENT QUESTIONNAIRE

No. Description

Respondent Methodology: Provide the detailed methodology for performing all services as described herein. What is your plan for performing the specified work including the process overview that explains what portion of work is using technology (including product identification) and what tasks are performed by a subject matter expert or translation specialist, or other? Provide a project plan that identifies workflows, timelines/milestones and project completion dates. Include any dependencies and/or assumptions that apply. Include a detailed response.

Response:

2 Communication. What communication practices are utilized to support your clients? Describe your overall methodology for communicating to key target audiences/stakeholder groups. Explain how work effort and progress will be monitored and communicated to FLVS.

Response:

Case Studies: Provide three (3) case studies demonstrating prior experience of similar size and scope.

Response:

4 Samples of Work. Below is a sample of course content for translation. Perform the English to Spanish translation of the provided text. The translated sample of work shall demonstrate the quality and expertise for the work to be performed.

Note: Sample pages for translation for each course have been attached below. Respondent shall include the translated samples with their submission in response to the RFQ.

Response:

Respondent acknowledges and agrees with all of the above assumptions, requirements and specifications as described.

☐ Yes or ☐ No

B. IMPLEMENTATION PLAN - MILESTONES/TIMELINE

The awarded contractor will provide a project delivery schedule for the course translations. The plan shall include key deliverables and milestone due dates. The development work for the project is anticipated to start upon issuance of purchase order or after contract execution. All deliverables must be completed and accepted by FLVS no later than thirty (30) calendar days from date of issuance of FLVS Purchase Order.

SAMPLE - Milestone/Deliverable Due Date Project Initiation/kick-off Upon issuance of FLVS Purchase Order Proof of Concept Date Delivery of fully translated course Date Final FLVS Acceptance Date

F LVS Annual Holiday Schedule Summer Break Week of July 4th

Labor Day First Monday September

Veterans Day November 11, 2025

Thanksgiving Break 4th Thursday November (W-F)

Winter Break December 19, 2025 to January 2, 2026

Martin Luther King Jr.

January 19, 2026

President’s Day February 23, 2023

Memorial Day Last Monday of May

Juneteenth June 19, 2026

FLVS Professional Learning Conference

Week of September 14, 2026

Respondent acknowledges and confirms their ability to meet or exceed the FLVS project schedule and workflows as described above.

☐ Yes or ☐ No

EXCEPTIONS AND ALTERNATIVES

Respondents may use this section to explain any exceptions taken to the scope along with an explanation and/or alternative offered.

Exception to FLVS Scope (please be specific) Explanation and/or Alternative Proposed

DEFINITIONS

Components of the average half-credit (one semester) course. Actual quantities may be higher or lower.

Approximately six Modules containing approximately 6 lessons per module (approximately 5 pages per lesson), ancillary documents including Develop Getting Started, Welcome Folder, Glossary, Collaboration, and toolbar (approximately 30 pages). Deliverables may also include but are not limited to graphics, images, and or custom images.

Components of a Lesson o Title o Identified objectives/learning targets o Instruction following commonly used curriculum framework directly aligned to identified educational standards and objectives/learning targets.

o Engaging and relevant page headers/titles o Consistent voice and tone (active voice) o Student engagement focus, including logical order of topics o Appropriate level of rigor per identified educational standards, including adequate scaffolding of skills and knowledge o Clear and concise text written at appropriate reading levels (identified in script) o Real world application where applicable o Graphic organizers and/or note-taking aids as appropriate o Dynamic delivery of content where appropriate (i.e. tabs, click to reveals, storybooks, audio) including text versions where applicable o Interactive practice(s) aligned to identified educational standards and objectives/learning targets o Visual elements complementing the instruction and student audience (i.e. images, infographics, diagrams, maps, charts, graphs, including alt text), including appropriate text lead-ins for these design elements.

o Video elements used to teach difficult concepts and engage student audience including text version o Glossary terms with definitions o Lesson recap o Authentic or traditional lesson level assessment aligned to lesson content and identified educational standards at appropriate level of cognitive complexity and difficulty o Assignments broken into clear, manageable steps (numbering of steps) o Rubrics are clear, concise, aligned to assessment instructions, and use a consistent format Module - Method for presenting materials in a course as a smaller unit of study that includes lessons that center around a common learning goal.

RFP01-2604720B01-DISLIF-XXXXXX

Cybersecurity Compliance. Describe in detail your organization’s cybersecurity compliance policies. Respondents must demonstrate policies in place to prevent a variety of common cybersecurity attacks. This includes, but is not limited to:

• Demonstrate use of current, supported and patched applications and libraries to minimize vulnerabilities in applications code as applicable.

• Demonstrate use of a proper error handling code to ensure that system information is not revealed.

Disclose any plugins, add-ons, third party tools or similar that will be included in your proposed solution.

General Requirements for self-hosted solution include:

Virtualization Requirements: Supports virtual machines running on currently supported version of virtualization software.

Operating System Requirements: Must use current operating systems for servers, desktops, tablets, phones. We will not accept bids without further security analysis using EOL operating systems.

Web Requirements: Must use current web hosting technologies with cybersecurity safeguards to include secure web application headers, TLS/SSL configurations and certificates, and port security.

Database Requirements: Must use currently supported database models and languages.

We will not accept bids without further security analysis using EOL database models or languages.

Maintenance and Support Requirements:

24x7x365 access to technical support with a maximum of 4-hour response time.

Maintenance updates to ensure FLVS is on the latest version and patched for known vulnerabilities.

General Requirements for Contractor-hosted solution include:

Contractor Compliance: Contractor has, and continues to maintain, an active SOC2 report that is available to FLVS upon request.

Availability: Dependent upon the business criticality that has been defined:

o Mission Critical Products – Minimum of 99.95% availability SLA (preferred 99.99%) o Business Critical Products – Minimum of 99.9% availability SLA (preferred 99.5%) o Important Business Products – Minimum of 99.5% availability SLA (preferred 99.9%) Data Retention Requirements (All):

Search Retrieval of Records: Allows FLVS to schedule and download backups of our data to fulfill retention requirements or:

o Allows FLVS to configure record retention lengths o Allows FLVS to search records o Allows FLVS to produce records including ones deleted by users that are within the retention period configured Access Controls:

o Integrates with Microsoft Single Sign-on/Identify and Access Management tools.

We will not accept bids from companies that do not work with Microsoft SSO without further security analysis.

Audit and Accountability:

o Provides an ability to audit and download the following types of events:

Access to PII or other sensitive data Actions taken by user with administrative access Failed access attempts When identification and authorization mechanisms are used Creation and deletion of privileged or system-level objects o Audit Logs Contain:

User ID Type of Event Date and Time Success or Failure Status Origin of Event Identify of the affected data, component, or resource

Configuration Management. Provides an ability to test and validate changes before deployment to the production environment.

Contingency Planning. Provides ability to:

o Reroute traffic and data to an alternate site in the event of an area-wide disruption or disaster o Backup and retain copies of FLVS configurations and data Maintenance: Contractor-hosted solution notifications are sent a minimum of seven (7) days prior to scheduled maintenance and will minimally include:

o Date, time, and duration of maintenance o Description of maintenance

EXHIBIT 1 - Exams by Course

EXHIBIT 2 – SAMPLE PAGES FOR TRANSLATION - BIOLOGY

Biology : Cell Reproduction : 03.03 Protein Synthesis

The Genetic Code

We keep saying that DNA and RNA contain information for building a protein molecule, but what is this genetic code and how is it interpreted?

Transcription uses base pairing to build a strand of complementary mRNA from a section of DNA. Translation will interpret the sequence of nitrogenous bases on the mRNA as instructions for assembling amino acids together to build a protein molecule. There are many different amino acids, 20 of which are commonly used to build proteins. The specific amino acids, and the order in which they are bonded together, determine the shape and properties of the protein.

If there are only four nitrogenous bases in a nucleic acid (DNA or RNA) molecule, how can those bases code for 20 different amino acids? The genetic code is read three bases at a time, where each set of three bases corresponds to a specific amino acid. Each set of three nitrogenous bases in an mRNA molecule that codes for a specific amino acid is called a codon.

Explore and learn about how the universal genetic code is transcribed and translated.

Record the information in your Guided Notes.

Now it's your turn! Review the universal genetic code.

Background Image: Diagram of DNA replication, transcription and translation. Top shows a DNA molecule as a double helix, an arrow points down to an exact copy of the the DNA molecule above

it. Then, a highlighted strand on DNA molecule has an arrow pointing down labeled transcription where mRNA, a red strand with three bases marked across the strand is next. The mRNA gets translated, showing a protein chain below, with amino acids in colorful circles starting with Met and ending with Pro.

With four different bases in RNA (A, U, C, and G) there are 64 possible codons (4 × 4 × 4 = 64) in the genetic code.

UNIVERSAL GENETIC CODE

TEXT VERSION

javascript:void(0);

javascript:void(0);

This chart lists all of the codons found on an mRNA molecule. Notice that some amino acids are specified by more than one codon. Also, notice that some are start or stop codons.

Steps to Read a Codon Chart

There are only 20 amino acids used in protein molecules, but some amino acids can be specified by more than one codon.

There are also codons that signal where to start and stop the assembly of the protein molecule. For example, the methionine codon AUG serves as a start codon for protein synthesis.

After a start codon, the base sequence is read three bases at a time until reaching a stop codon. At the point of a stop codon, the protein synthesis stops because the new protein molecule is complete.

The genetic code, including this process to code for amino acids, is used by nearly every organism on Earth. Scientists refer to this as the "universal genetic code."

The codon chart can be used to determine the corresponding amino acids when given a codon sequence. Remember, a codon is a set of three nitrogenous bases in an mRNA sequence. Each codon codes for a specific amino acid, which is the building block of proteins.

1. Get Your mRNA Sequence: Look at the mRNA sequence you want to translate. It will be made up of the letters A, U, C, and G.

2. Split into Codons: Break the mRNA sequence into groups of three letters (codons).

For example, if your sequence is AUGCCU, you have two codons: AUG and CCU.

3. Find the First Base: Locate the first letter of your first codon on the left of the codon chart. This determines which row the correct amino acid is in. For example, if your first codon is AUG, find the letter "A" on the left side.

4. Find the Second Base: Next, look for the second letter of the codon on the top of the chart. This determines which column the correct amino acid is in. For AUG, the second letter is "U."

5. Locate the Third Base: Finally, find the third letter of the codon on the right of the codon chart. This determines which section the correct amino acid is in by following this line across the column. For AUG, the third letter is "G."

6. Read the Chart: Now, look where the row (from the first letter) and column (from the second letter) intersect with the third letter. This will tell you which amino acid corresponds to that codon. For AUG, you'll find "Methionine" or "Start Codon."

Working with a codon chart will require a lot of practice until you are familiar with the process. Play this video for more details and examples on using the codon chart if you need it.

How to Use a Codon Chart Text Version

Hello, biology students. In this short video, we'll be discussing how to read a codon chart with some examples and an opportunity to practice this important skill.

[On screen is a full codon chart with five rows and six columns with the first column, starting in the second row as U, C, A, G, with a letter in each cell, then the top row, starting in the second column showing U, C, A, G in each cell, and the right-most, column showing all four letters U C A G for each cell starting in the second row. Each cell shows four words vertically that corresponds to the overlapping of the letters U C A G in the following manner:

Alanine: GCU, GCC, GCA, GCG; Arganine: CGU, CGC, CGA, CGG; Arginine: AGA, AGG;

Asparagine: AAU, AAC; Aspartate: GAU, GAC; Cysteine: UGU, UGC; Glutamate: GAA, GAG;

Glutamine: CAA, CAG; Glycine: GGU, GGC, GGA, GGG; Histidine: CAU, CAC; Isoleucine: AUU, AUC, AUA; Leucine: UUA, UUG, CUU, CUC, CUA, CUG; Lysine: AAA, AAG; Phenylalanine: UUU, UUC; Proline: CCU, CCC, CCA, CCG; Serine: UCU, UCC, UCA, UCG, AGU, AGC; Threonine: ACU, ACC, ACA, ACG; Tyrosine: UAU, UAC; Tryptophan: UGG; Valine: GUU, GUC, GUA, GUG; Start

Codon; Methionine: AUG; Stop Codon: UAA, UAG, UGA.]

First, let's review. mRNA contains the instructions to assemble amino acids, and amino acids are the monomers, or smaller components, that build proteins.

https://lti.flvsgl.com/flvs-cat-content/lkch8f0nfk4jo8buqmtt91hv92/flvs-cat-session/biology1_v27/module03/lesson03/textversion/textversion_02.htm https://lti.flvsgl.com/flvs-cat-content/lkch8f0nfk4jo8buqmtt91hv92/flvs-cat-session/biology1_v27/module03/lesson03/textversion/textversion_02.htm

A codon is a set of three nitrogenous bases in the mRNA. So if we are given an mRNA sequence, we can use this codon chart to determine the corresponding sequence of amino acids. Let's take a look at our first example.

[Text on screen appears: Example 1: mRNA: AUGCAUUGA]

When we are looking at this mRNA sequence, the first thing we need to do is to break it up into codons. Remember, a codon is a set of three nitrogenous bases represented by the letters U, C, A, and G. So our first codon—the set of the first three letters is AUG, the next is

CAU, and the third is UGA.

[On screen are the letters AUG, CAU, and UGA.]

Now we're ready to use our codon chart to determine the amino acid that corresponds to our first codon, AUG. Our first base, or first letter, is A so we look for an A on the left side, and we see that the A is the third one down on the left side. That means that our corresponding amino acid for the codon, AUG, must be in this section.

[On screen, the letter A in AUG is highlighted, in the table, the letter A on the left-hand column is circled, and the entire fourth row is circled.]

Our next letter is U, so we look along the top for U and the U is in the first column. So that means that we are narrowing our options down to the first column. Actually, we've narrowed it down to the box where these two overlap.

[On screen, the letter U in AUG is highlighted, in the table, the letter U on the top row is circled, and the entire second column is circled. The overlap of A and U cell is circled, encircling the four words Isoleucine, Isoleucine, Isoleucine, and Methionine.]

Finally, our third letter in the first codon is G. We locate the G over on the right side of the codon chart, but we've already narrowed it down to the A section from the first letter. So we use this G, and we follow that across to locate our amino acid, which is methionine. AUG is actually a special codon called the start codon.

[On screen, the G in the fourth row, sixth column of the cell is circled, and an arrow points back to the word Methionine in the fourth row, second column.]

This is where protein synthesis begins, and in the cell, this special code tells the ribosome to start here. Next, we'll go through this process with the next codon, CAU.

[On screen, the CAU from the example is highlighted.]

So where are we going to look first? We're going to look on the left side for this first letter C.

[On screen, in the table, the letter C on the left-hand column is circled, and the entire third row is circled.]

So what's next? The second letter of our codon is A. So we look for the A along the top.

There it is. So now we're looking in the second set of the third column. So I have four amino acids here in this box where they overlap.

[On screen, the letter A in the top row is circled, and the entire third column is circled. The overlap of C and A cell is circled, and the four overlapping words are Histidine, Histidine, Glutamine, and Glutamine.]

Practice makes perfect when it comes to reading codon charts, use the activity below for more practice.

But how do you know whether it is histidine or glutamine? We have one more nitrogenous base in our codon. The third letter, which is U. So what do we do with this U? We look over on the right section, in that section that we've already identified with the first letter and here it is. We follow that U across and our amino acid for the codon CAU is histidine.

[On screen, the U in the cell in the third row, sixth column is circled, and an arrow points back to the word Histidine in the third row, fourth column.]

Now you're ready to give it a try on your own. Our last codon is UGA. Go ahead and pause the video and use the codon chart to determine the correct amino acid for UGA.

[On screen, the UGA from the example is highlighted.]

Alright. So let's take a look at this last codon UGA. First, we look on the left side of the chart for the first letter, U. Then we locate the G along the top, and finally the A on the right side.

[On screen, the U in the first column and entire second row are circled, then the fifth, or G column is circled, and finally the A in the cell in the second row, sixth column is circled, and an arrow points back to the word Stop in the second row, fifth column.]

Did you get stop? UGA is another special codon called the Stop codon. This is a message that tells the ribosome that this is the end of the mRNA sequence, and that this chain of amino acids is complete.

What if there was a change in the mRNA sequence or a mutation that caused our last codon to be UAA instead of UGA? Take a moment and find the amino acid for UAA in our chart.

[On screen, the word Stop located in the second row, fourth column is circled.]

You'll notice that UAA also codes for the stop codon. In fact, there are multiple stop codons and many examples of repeated amino acids through the codon chart.

[On screen, the other Stop words are circled.]

This means that there can sometimes be a change or mutation in the original DNA sequence or the corresponding mRNA sequence, and it might not cause a noticeable change in the final resulting amino acid sequence or in the protein, which means that this protein will still function normally.

One last important point: the components that make up our genetic material as well as this codon chart are the same in almost every living organism on earth. For example, CUA codes for leucine, GCA corresponds to alanine, whether an organism is a single cell of bacteria, a fungus, or a plant, or whether it's a jellyfish or a human. In science, we call this the universal genetic code. I hope this video has helped you to gain a better understanding of how to use a codon chart. Thanks for watching.

Read each question and review the best option.

GENETIC CODE — TEXT VERSION

Slide 1 Image of: Codon chart in a circle format, showing the bases in the middle and amino acids circled around.

Use the codon chart above to translate the following mRNA strand to amino acids.

AUG-CGC-UUA-CCC-UGA

Start (MET)-GLU-LEU-Ala-Stop

ARG-LEU-PRO-MET-STOP

Start (MET)-ARG-LEU-PRO-Stop

Show Answer for Slide

Slide 2 Image of: Codon chart in a circle format, showing the bases in the middle and amino acids circled around.

Now, work backward. What would be the strand of DNA that could code for the following protein sequence?

Amino Acids: Cysteine - Tyrosine

UGU-UAU

GGC-GUA

ACA-ATA

Show Answer for Slide

Slide 3 Image of: Codon chart in a circle format, showing the bases in the middle and amino acids circled around.

Last one, tRNA brought the following amino acids with their attached anticodon. What would be the correct mRNA codon?

Cysteine (ACA) – Tyrosine (AUA)

ACA-AUA

TGT-TAT

UGU-UAU

Show Answer for Slide

EXHIBIT 3 – SAMPLE PAGES FOR TRANSLATION – ALGEBRA

Systems Containing Horizontal and Vertical Inequalities

Horizontal lines are lines where the value stays the same for all values of within the domain. The value of is constant and independent of the value of Vertical lines are lines where the value stays the same for all values of the range.

Horizontal inequalities are similar to horizontal lines except that instead of the value of staying constant, the solution set will include all values of either greater than the or less than depending on the inequality. If the inequality included the equal sign, the value will also be in the solution set. In a similar way, vertical inequalities have a vertical line as the boundary line and are shaded to the left or right of the line.

y x y

x. x y y a a, a

Horizontal Lines Vertical Lines

Horizontal lines have a slope of zero.

Horizontal lines always cross the y-axis.

Equations are in the form where is any real number and is referred to as the y-intercept.

Horizontal lines are parallel to each other.

Horizontal lines are perpendicular to vertical lines.

y = b, b

Vertical lines have an undefined slope.

Vertical lines always cross the x-axis.

Equations are in the form where is any real number and is referred to as the x-intercept.

Vertical lines are parallel to each other.

Vertical lines are perpendicular to horizontal lines.

x = a, a x –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 y

–6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 x –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 y

–6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1

Inequalities Involving Horizontal and Vertical Lines x > 4 x < 4 y > 4 y < 4 x –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 y

–8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 x –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888 y

–8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 x –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 y

–8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 x –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 y

–8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1

ALGEBRA I : SYSTEMS OF EQUATIONS AND INEQUALITIES : 03.04 SYSTEMS OF LINEAR INEQUALITIES

Graphing an inequality where the boundary line is horizontal or vertical is similar to graphing an equation of a horizontal or vertical line. The only differences are that the line may be dashed and shading will be involved.

Graph the inequality y ≥ −4.

Step 1 Graph the boundary line.

The inequality is comparing and so it is a horizontal line passing through on the y-axis.

The line is solid because the inequality sign is greater than or equal to.

y ≥ −4 y −4, −4 x –8–8–8 –6–6–6 –4–4–4 –2–2–2 222 444 666 888 y

–8–8–8

–6–6–6

–4–4–4

–2–2–2

Step 2 Shade on the appropriate side of the line.

Since all y-axis values greater than or equal to will satisfy the inequality, the shading is above the line.

−4

–8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888

–8–8–8

–7–7–7 –6–6–6 –5–5–5

–4–4–4 –3–3–3

–2–2–2 –1–1–1

Important

It is important to remember that all points in the shaded region are solutions to the inequality.

Three points are plotted on the graph. Two points, and are in the shaded region, so they are part of the solution set. The ordered pair, is on the solid boundary line of the solution region and thus is also part of the solution set. If the line were dashed, the points on the line would not be part of the solution set.

(−2, 8) (5, 1), (4, −4), –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888

–8–8–8

–7–7–7 –6–6–6 –5–5–5

–4–4–4 –3–3–3

–2–2–2 –1–1–1

Graph the system of inequalities and x < 6 y ≥ 3.

Example

Step 1 Graph the first inequality.

The inequality is a vertical line that passes through

The line is dashed because the inequality sign is less than.

x < 6

(6, 0).

–4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888 999 101010 111111

–7–7–7

–6–6–6

–5–5–5

–4–4–4

–3–3–3

–2–2–2

–1–1–1

Since all x-axis values less than or equal to satisfy the inequality, the shading is to the left of the line.

–4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888 999 101010 111111

–7–7–7

–6–6–6

–5–5–5

–4–4–4

–3–3–3

–2–2–2

–1–1–1

Step 2 Graph the second inequality on the same coordinate plane.

The inequality is a horizontal line that passes through

The line is solid because the inequality sign is greater than or equal to. Since all y-axis values greater than or equal to will satisfy the inequality, the shading is above the line.

(0, 3).

–4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888 999 101010 111111

–7–7–7

–6–6–6

–5–5–5

–4–4–4

–3–3–3

–2–2–2

–1–1–1

Once a system of inequalities is graphed, it can be determined if a certain point falls within the solution set of that graph. Simply graph each inequality and observe the shaded region. If the point is in the region that overlaps between the inequalities, then the point is a part of the solution set.

Graph the system of inequalities.

x ≥ −2 y < − 1 2 x + 3

Graph the first inequality.

The inequality is a vertical line passing through the point

Since the sign is greater than or equal to, the line is solid.

Since all x-axis values greater than or equal to satisfy the inequality, the shading is to the right of the line.

x ≥ −2

(−2, 0).

–10–10–10 –8–8–8 –6–6–6 –4–4–4 –2–2–2 222 444 666 888 101010 121212

–12–12–12

–10–10–10

–8–8–8

–6–6–6

–4–4–4

–2–2–2

101010

Graph the second inequality on the same coordinate plane.

The equation is in slope-intercept form, so use the y-intercept, and slope, to graph.

The inequality is in slope-intercept form with a less than sign, so the line is dashed and the shading is below the line.

y < − 1 2 x + 3

3, − 1 2 , –10–10–10 –8–8–8 –6–6–6 –4–4–4 –2–2–2 222 444 666 888 101010 121212

–12–12–12

–10–10–10

–8–8–8

–6–6–6

–4–4–4

–2–2–2

Determine if the ordered pair is in the solution set.(6, −4)

The point is in the overlapping areas and thus is in the solution set.

This can also be verified by checking algebraically if the point satisfies the system of linear inequalities.

(6, −4) x ≥ −2 y ≤ − x + 3

6 ≥ −2 ✓ −4 ≤ −

(6) + 3

−4 ≤ −3 + 3

−4 ≤ 0 ✓

–10–10–10 –8–8–8 –6–6–6 –4–4–4 –2–2–2 222 444 666 888 101010 121212

–12–12–12

–10–10–10

–8–8–8

–6–6–6

–4–4–4

–2–2–2

The words above or below the line are not used with vertical inequalities. Instead of above or below, the shading is either to the left or right of the line. Values on a number line increase to the right and decrease to the left. The shading of vertical inequalities follows the same pattern. This means right can be thought of as above the line and left as below the line.

Graph the solution to the system of inequalities.

y ≤ −3 y > 2x − 1

Graph the first inequality.

The inequality is a horizontal line that passes through

The line is solid because the inequality sign is less than or equal to. Since all y-axis values less than or equal to will satisfy the inequality, the shading is below the line.

y ≤ −3

(0, −3).

−3

–9–9–9 –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888 999

–9–9–9 –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1

Graph the second inequality on the same coordinate plane.

The equation is in slope-intercept form, so use the y-intercept, and slope, to graph.

The inequality is in slope-intercept form with a greater than sign, so the line is dashed and the shading is above the line.

y > 2x − 1

−1, 2, –9–9–9 –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555 666 777 888 999

–9–9–9 –8–8–8 –7–7–7 –6–6–6 –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1

Graph the inequality y ≤ 8.

Check

Step 1 Graph the boundary line.

The inequality, compares and so it will be a horizontal line passing through on the y-axis.

The inequality symbol, is used, so the line is solid. Graph a solid, horizontal line passing through the point y ≤ 8, y 8, 8

(0, 8).

Step 2 Shade on the appropriate side of the line.

The line is solid because the inequality sign is less than or equal to. Since all y-axis values less than or equal to will satisfy the inequality, the shading is below the line.

–6 –5 –4 –3 –2 –1 1 2 3 4 5 6

–3

–2

–1

Practice

Graph the system of inequalities and x ≥ 3 y < −2.

Check

Step 1 Graph the first inequality.

The inequality, compares and so it is a vertical line passing through on the x-axis.

The inequality symbol, indicates that the line is solid. Graph a solid, vertical line passing through the point

The inequality, means that is greater than or equal to so shade the area to the right of the graphed line.

x ≥ 3, x 3, 3

(3, 0).

x ≥ 3, x 3, Step 2 Graph the second inequality on the same coordinate plane.

The inequality, compares and so it is a horizontal line passing through on the y-axis.

The inequality symbol, indicates that the line is dashed. Graph a dashed, horizontal line passing through the point

The inequality, means that is less than or equal to so shade the area below the graphed line.

y < −2, y −2, −2

(0, −2).

y < 2, y

2, –5–5–5 –4–4–4 –3–3–3 –2–2–2 –1–1–1 111 222 333 444 555

–5–5–5

–4–4–4

–3–3–3

–2–2–2

–1–1–1

PracticeALGEBRA I : SYSTEMS OF EQUATIONS AND INEQUALITIES : 03.04 SYSTEMS OF LINEAR INEQUALITIES

Solicitation Number: RFQ01-2604741B01-TRANS-XXXXXX

IV. PRICING SHEET

MUST BE COMPLETED AND SIGNED BY EACH VENDOR FOR QUOTE SUBMITTAL

Company Name: Telephone Number:

Primary Contact: Email Address:

QUOTE: CURRICULUM TRANSLATION SERVICES

RFQ01-2604741B01-TRANSL-XXXXXX

Section I - Fixed Fees: Provide All Inclusive Fixed Fees Proof of Concept $ Per Course for complete course translation $ Entire Project (including proof of concept and two courses fully translated)

Section II – Maximum Rates for Services: Provide the all-inclusive, not to exceed rates (per hour/per word) for each of the types of services listed as applicable. Additional lines may be added if necessary.

These rates are used to determine the all-inclusive Per Course Fixed Fee provided above and may be used for additional work effort.

Description Unit of Measure

Unit Cost Extended Cost

AI/Software Assisted Translation Per Word $ $ Human Post-Editing (including QA/Proofreading Process)

Per Hour $ $

Other (explain) Per $ $

Section III – : Tiered Pricing for bulk translation. This may be provided in addition to the above as an cost savings option as an alternative.

Description Unit of Measure Cost 0-10,000 words Tier 1 Price $ 10,001 - 50,000 words Tier 2 Price $ 50,001 and above Tier 3 Price $

Section IV - Alternative Pricing Model: May be provided in addition to the above (not as a replacement) alternative services that may be relevant for consideration.

Additional Services (optional): Respondents shall provide rates for additional related services not specified in the scope of services. For additional available services, provide a detailed description of service along with associated rates. FLVS shall at its discretion exercise the right to purchase any proposed additional services as needed. Additional services proposed shall not factor into the evaluation process. Respondent shall specify any additional services offered.

• If any services, functions, or responsibilities not specifically described in this RFQ are necessary for the proper performance and provision of the Services, they shall be deemed to be implied by and included within the scope of the Services to the same extent and in the same manner as if specifically described herein.

• Unit price is “per item” price

• Unit price shall be used to determine correct extended price if calculation error is found

• Extended price should be computed as Estimated Number of Hours multiplied by the proposed

Unit Price

• All quantities are estimated and could be higher or lower at the discretion of FLVS in accordance with the Statement of Work

• FLVS is a tax-exempt entity, pricing shall not include any sales tax

• Upon award, the Contractor agrees that FLVS can purchase additional quantities for duration of the agreement under the terms and prices quoted in this RFQ or a minimum of 180 calendar days (whichever is greater).

• There is no travel contemplated for this RFQ.

Additional information regarding your quote (writing sample, project plan, etc.) may be submitted in a separate document.

P-Card Acceptance Respondent shall accept payment via P-Card (with no additional Fees) ☐ Yes ☐ No (Check One)

I agree to abide by all conditions of this offer and certify that I am authorized to sign this offer on behalf of the company and that the company is in compliance with all requirements of the Request for Quote.

Company FEIN:_____________________

Signature: _______________________ Date: _____________

Typed Name & Title__________________________________