1. Purchase Description.docx

PURCHASE DESCRIPTION

Custom Lumencor Spectra III light sources for Zeiss microscopes, FICCF, NINDS

1. Statement of Need and Purpose: Flow and Imaging Cytometry Core Facility (FICCF) needs to purchase four (4) custom multispectral fluorescence solid-state LED/Laser light source units (model Lumencor Spectra III light engine or equivalent) for the existing Zeiss epifluorescence microscopes in FICCF to enable full light spectrum fluorescence imaging spanning the ultraviolet to near-infrared (395-740 nm) fluorescence excitation range.

2. Background Information and Objective: The National Institute of Neurological Disorders and Stroke (NINDS) Flow and Imaging Cytometry Core Facility (FICCF) provides routine use of conventional flow cytometry, imaging cytometry, fluorescence-activated cell sorting (FACS) and in situ multispectral microscope imaging technology and application techniques to assay a multitude of biological properties at the molecular, subcellular, cellular and tissue levels, as specified by NINDS and other NIH intramural investigators. To this end, the NINDS FICCF owns flow cytometry and imaging equipment which requires periodic upgrades, replacements and/or addition of new state-of-the-art equipment featuring new bioassay technologies and techniques to ensure full operational performance to optimally support continuously evolving research requirements performed at the Facility. FICCF currently needs to purchase 4 custom multispectral fluorescence solid-state LED/Laser light source units (model Lumencor Spectra III light engine or equivalent) to enable full light spectrum fluorescence imaging spanning the ultraviolet to near-infrared (395-740 nm) fluorescence excitation range, in order to optimally support multiplex immunohistochemical high content biomarker screening applications using multispectral fluorescence imaging applications routinely carried out at this Core Facility.

3. Generic Name of Product: Custom Lumencor Spectra III multispectral light sources

4. Purchase Description: Four (4) custom Lumencor Spectra III multispectral LED/Laser light source units (or equivalent) are needed with the technical specifications listed in the salient characteristics section below.

5. Salient characteristics: The multispectral light source engine must include 8 independently controlled solid state LED/Laser light sources with excitation peaks (395 nm, 440 nm, 475 nm, 550 nm, 585 nm, 635 nm, 680 nm, 740 nm), internal bandpass (BP) blocking filters (395/25 nm, 434/21 nm, 485/25 nm, 540/24 nm, 590/20 nm, 631/28 nm, 680/10 nm, 730/40 nm), and power outputs of at least 250 mW per excitation line), as listed in the technical specification table (Table 1) below. Each light source must also include serial (USB B and RS232) and TTL (DB15) control ports, onboard microprocessor, and 3 mm liquid light guides (LLGs), as listed in Table 1, for continuous power monitoring and stabilization and uniform field illumination to optimally support multiplex immunohistochemical high content biomarker screening applications using multispectral fluorescence imaging applications routinely carried out at this Core Facility. Full list of technical specifications that fully meet to meet FICCF requirements are shown in Table 1 below. Each light source must also be specified to provide optimal performance for stable white light illumination output, when all 8 excitation lines are turned ON, for at least 20,000 work hours.

Table 1. Light source specification table of required custom technical features.

6. Quantity: 4 Units

7. Delivery Date: Within 90 days of purchase.

8. Period of Performance: N/A

9. Options: N/A image1.emf