Project Grant R44HG014736
- This Project Grant award from the National Institutes of Health's National Human Genome Research Institute provided $768,678 to Molecular Assemblies, Inc. to develop a fully enzymatic oligonucleotide synthesis cycle using engineered template-independent polymerases and a novel phosphate dNTP blocking group. The award supports the goals of the Human Genome Research program (CFDA 93.172) to accelerate genome research and its application to human health. Specifically, the awardee will engineer...
- The National Institutes of Health National Human Genome Research Institute awarded $484,634 to Dnali Data Technologies, Inc. on September 1, 2026, under the Human Genome Research program (CFDA 93.172) to develop and commercialize a platform for unbiased amplification of single-stranded DNA oligonucleotides. Current methods for producing and amplifying single-stranded DNA are inefficient, biased, and fail to maintain sequence fidelity or representation, particularly for long templates, very short...
- The National Science Foundation awarded Dinya DNA, Inc. a $1.25 million cooperative agreement on August 1, 2026, to commercialize an automated, enzymatic DNA synthesis platform under the NSF Technology, Innovation, and Partnerships program (CFDA 47.084). The recipient will develop and optimize a hierarchical assembly approach that synthesizes long, complex DNA sequences using small double-stranded DNA precursors assembled enzymatically in exponential fashion, replacing traditional...
- The National Institutes of Health National Human Genome Research Institute awarded Yale University $2.618 million on July 6, 2026, under the Human Genome Research program (CFDA 93.172) to develop advanced computational tools for de novo genome assembly and assembly-based genomic analysis. The project, titled "Toward Comprehensive Genomic Analysis Through De Novo Genome Assembly," will produce three deliverables. First, the recipient will develop a real-time assembly algorithm that...
- The National Institutes of Health National Human Genome Research Institute awarded New York University $466,125 on May 18, 2026, under CFDA 93.172 (Human Genome Research) to support cross-species analysis of regulatory genomic function using synthetic regulatory genomics. The award funds development and application of the BIG-IN genome engineering technology to characterize regulatory DNA function across a phylogenetic tree. The recipient will analyze model genomic loci in mouse embryonic stem...
- The National Human Genome Research Institute (NHGRI) awarded a $457,920 Project Grant (CFDA #93.172 - Human Genome Research) to The Broad Institute, Inc. to develop a deep reinforcement learning framework for haplotype assembly. Haplotype assembly is critical for understanding human population genetics and disease, as it reconstructs the combination of alleles on maternally and paternally inherited chromosome copies. The Broad Institute, a renowned biomedical and genomic research organization,...
- The National Institutes of Health National Human Genome Research Institute awarded New York Genome Center, Inc., $2,677,663 on June 22, 2026, under the Human Genome Research program (CFDA 93.172, Assistance Listing designation R01HG014032) to develop next-generation spatial multi-omics technologies. The recipient will design a cost-effective, user-friendly platform for spatial multi-omic profiling that integrates chromatin states, transcriptional activity, and other modalities simultaneously...
- The National Human Genome Research Institute (NHGRI), under the Human Genome Research Federal Grant Program (CFDA 93.172), awarded a $384,418 Project Grant to Xgenomes Corp. on September 25, 2024 to develop a revolutionary DNA sequencing platform. The project aims to create a super-resolution single-molecule sequencing technology that can read short sequence "words" rather than individual nucleotides, enabling one or more orders of magnitude improvements in accuracy and cost over...
- The National Institute of General Medical Sciences awarded Proteovista LLC $306,151 on June 2, 2026, under the Biomedical Research and Research Training program (CFDA 93.859) to develop ClusterBuild, a platform for cost-effective synthesis of large biosynthetic gene clusters up to 100 kilobases in size. ClusterBuild combines DNA microarrays with peptide nucleic acid (PNA)-based error correction and isothermal assembly to enable high-fidelity synthesis of biosynthetic gene clusters derived from...
- The National Human Genome Research Institute awarded $396,170 to Syntaxisbio Inc. on September 17, 2025, under the Human Genome Research program (CFDA 93.172) to develop novel technologies for cellular age profiling and validation. Syntaxisbio will develop genetic PUFs (Physically Unclonable Functions)—unique, robust genetic identifiers embedded within cell lines using molecular barcoding and CRISPR-induced DNA repair mechanisms—to enable accurate tracking of cell line provenance and age. The...
The National Institutes of Health National Human Genome Research Institute awarded $1,077,022 to Bacstitch DNA Inc. on September 1, 2026, under the Human Genome Research program (CFDA 93.172) to develop and optimize a hierarchical in vivo DNA assembly platform for high-throughput construction of long DNA sequences exceeding 80 kilobases. Bacstitch DNA will enhance its E. coli-based assembly platform by transitioning from sequential to hierarchical assembly workflows, integrating orthogonal bacterial conjugation systems with conditional origins of replication to streamline workflows, reduce hands-on time, and improve accuracy and throughput. The platform combines bacterial conjugation, in vivo DNA cutting, and homologous recombination to enable seamless, modular assembly without in vitro steps, sequence scars, or refactoring requirements. Specific optimization efforts include scaling the hierarchical assembly workflows and implementing barcoded arrays for high-throughput sequence validation. The work addresses limitations in existing DNA assembly methods such as Gibson and Golden Gate assembly, which perform poorly on longer and complex sequences, and yeast-based assembly, which suffers from low yields and long turnaround times. The enhanced platform targets applications in biosynthetic pathway engineering, synthetic chromosome design, and combinatorial genetic library optimization. Place of performance is San Carlos, California. The award period runs from September 1, 2026, through August 31, 2028.
Mod # | Description | ReasonForModification | Federal Obligation | Date |
|---|---|---|---|---|
| Not listed | $1.1m | 9/3/26 |