This $406,500 Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research Federal Grant Program (CFDA 93.172), aims to advance next-generation mass spectrometry-based sequencing (NGMS-SEQ) methods for comprehensive, unbiased sequencing and quantitative mapping of RNA modifications. The key objectives are to: 1) Increase the throughput of NGMS-SEQ to directly sequence various physiologically relevant tRNA samples, and 2) Enhance the...
The National Human Genome Research Institute (NHGRI), under the Federal Grant Program "Human Genome Research" (CFDA 93.172), awarded a $407,291 Phase I Small Business Innovation Research (SBIR) grant to Electronic Biosciences, Inc. to develop a nanopore-based protein sequencing platform. The project aims to create a high-accuracy, label-free nanopore reader capable of direct, single-molecule protein sequencing. This novel technology would enable proteome-wide profiling, biomarker...
The National Human Genome Research Institute (NHGRI), under the $384,418 Project Grant award R43HG013911, is supporting the development of a revolutionary DNA sequencing platform by Xgenomes Corp. The project aims to create a super-resolution single-molecule sequencing technology that can read short sequence "words" rather than individual nucleotides, enabling higher accuracy and lower costs compared to existing sequencing methods. The key technical objectives include expanding the...
The National Human Genome Research Institute (NHGRI) awarded a $2,040,824 Project Grant (CFDA 93.172 - Human Genome Research) to the University of Massachusetts (UMass) to develop an enzymatic system for site-specific incorporation of modified RNA bases. The research aims to build on advances in co-immobilized flow transcription to enable the synthesis of RNA containing multiple, strategically placed base modifications. This capability will facilitate fundamental studies of RNA translation,...
This Project Grant award, valued at $215,088.00 and funded by the National Institutes of Health's National Human Genome Research Institute (CFDA 93.172 - Human Genome Research), supports the development of an "Exhaustive De Novo Sequencing of Every RNA in a Sample by a Layer-by-Layer Mass Spectrometry Ladder Intensity Approach" at Directseq Biosciences, Inc. The project aims to 1) further develop a next-generation mass spectrometry-based RNA sequencing method that can comprehensively...
The National Human Genome Research Institute (NHGRI), under the Human Genome Research Federal Grant Program (CFDA 93.172), awarded a $3,377,867 Project Grant to Survey Genomics, Inc. on September 19, 2024. The grant supports the development and commercialization of the CYTOPE-DROP platform, an innovative approach for spatially-resolved single-cell multiomics analysis. Key features of the CYTOPE-DROP technology include: 1) high-throughput capacity to localize individual cells and obtain their...
This Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research program (CFDA 93.172), provides $639,134 to the University of California, Los Angeles (UCLA) to develop new methods for globally identifying protein and ribonucleoprotein (RNP) interactions with nascent, unspliced RNA in human cells. The goal is to produce comprehensive binding site maps for several key molecules involved in RNA splicing, including RBFOX/LASR complexes, the U1...
The National Human Genome Research Institute (NHGRI) awarded a $136,661 Project Grant (CFDA 93.172 - Human Genome Research) to Brigham & Women's Hospital Inc. on September 23, 2024. The grant supports the development of an innovative high-throughput PADIT-SEQ technology to study the binding affinity between transcription factors and DNA. The research aims to uncover fundamental principles governing transcription factor-DNA interactions, advance understanding of the regulatory genome, and...
The National Human Genome Research Institute (NHGRI) awarded a $409,699 Project Grant (CFDA 93.172 - Human Genome Research) to the Regents of the University of Michigan to integrate liquid chromatography-mass spectrometry (LC-MS/MS) and nanopore sequencing platforms. The goal is to enable the direct and quantitative detection of RNA modifications in tRNA, rRNA, and mRNA. The University of Michigan is collaborating with researchers at the University of Oregon and Northeastern University on this...
The National Institute of General Medical Sciences (NIGMS) awarded a $350,000 SBIR Project Grant (CFDA 93.859 - Biomedical Research and Research Training) to Quantitative Biosciences, Inc. (QBiSci.com), a for-profit company in San Diego, CA. The grant aims to develop a neural network-accelerated DNA and RNA sequence processing node, called CLEDGESEQ, for distributed next-generation sequencing (NGS) data processing at the point of sequencing. The key objectives are to: 1) Develop neural...
The National Human Genome Research Institute (NHGRI) awarded a $383,482 Project Grant under the Human Genome Research federal grant program (CFDA 93.172) to Electronic Biosciences, Inc., a small biotechnology company in San Diego, California. The grant, awarded on June 6, 2025, aims to develop SPINSEQ, an innovative platform designed to deliver highly precise and accurate RNA sequencing. The project will focus on maintaining RNA integrity and ensuring precise sequencing, with a particular emphasis on detecting critical RNA modifications such as N6-methyladenosine, 5-methylcytosine, and pseudouridine. These modifications are crucial for understanding gene expression regulation and disease mechanisms. The grant will fund the development and optimization of the SPINSEQ platform's functionality, demonstration of its multiplexing capabilities for high-throughput sequencing, and performance testing on various RNA samples. The project will run through May 31, 2026, with the goal of establishing SPINSEQ as a groundbreaking tool for RNA research by addressing the limitations of existing technologies and delivering a more accurate, reliable, and scalable solution for both transcriptomic and epitranscriptomic studies.