Structural profiling of native proteins using fluorosequencing, a single molecule protein sequencing technology
Ended Project Grant Awarded Last action
- Award ID
- R43HG012561
- Recipient
-
Erisyon Inc. UEI XBCYESFSZJM6
- Awarding agency
-
National Human Genome Research Institute
National Institutes of Health, Department of Health and Human Services
- Obligated
- $433,818
Award facts
- Assistance listing
- 93.172 Human Genome Research
- Completion date
- Place of performance
- Austin, TX 78752, USA
Award details come from USAspending.gov.
Description
- Abstract
- Despite the wealth of accurate and high sensitive data from next-GEN DNA sequencing technologies, understanding the impact of the observed genomic mutations or alterations in the transcript (such as from splicing or transcriptional errors) on the protein structure and function is often unclear. To complicate this understanding are observations, where small abundant proteoforms can cause toxic intracellular aggregates. This link between genome/transcriptome to protein structure is unclear, because methods for characterizing protein structures often involve recombinant protein production and the use of analytical techniques that perform ensemble analysis. In this proposal, we introduce fluorescent protein painting, a workflow for obtaining protein's structural profiles by expanding the erisyon's existing fluorosequencing (single molecule protein sequencing) technology. The steps in workflow are - (a) reversible, covalent capture of natively folded proteins, (b) fluorescently labeling select surface exposed residues, (c) releasing, denaturing and digesting the proteins before (d) reacting the internal residues amino acid residues with second set of fluorophores. Performing fluorosequencing on this population of peptides will result in generation of protein structural profile or foot-prints on millions of individual peptides. Since the proteins are directly obtained from their native environment and the method can discriminate different protein conformations at single molecule sensitivity, we will have a better understanding of the impact of genomic mutations on the functional state of the biological sample.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $216,909 | |
| Original award | $216,909 |
Quick answers
Who received award R43HG012561?
R43HG012561 was awarded to Erisyon Inc. by National Human Genome Research Institute (National Institutes of Health) on September 22, 2022.
How much is award R43HG012561 worth?
$433,818 has been obligated on R43HG012561.
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