Broadly accessible technologies for single-cell joint analysis of transcriptome and epigenome
Ended Project Grant Awarded Last action
- Award ID
- R41MH128993
- Recipient
-
Epigenome Technologies, Inc. UEI LS7JQN77JKQ1
- Awarding agency
-
National Institute of Mental Health
National Institutes of Health, Department of Health and Human Services
- Obligated
- $1,404,953
Award facts
- Assistance listing
- 93.242 Mental Health Research Grants
- Completion date
- Place of performance
- California, USA
Award details come from USAspending.gov.
Description
- Abstract
- Histone modifications carry rich information of cellular memory and gene regulatory mechanisms. Single cell analysis of histone modification in conjunction with transcriptome could recover this critical layer of cell identity and help to dissect the cellular and molecular composition of complex tissues such as the brain. We recently developed an ultra-high throughput single cell multi-omics assay, paired-tag, that can jointly map transcriptome and histone modifications from up to a million single cells in parallel. In order to translate this novel technology to the marketplace and to advance the goals of the brain initiative, we propose to optimize and further develop paired-tag to enable its broad use in the research community. Specifically, we will develop paired-tag kits to allow for general accessibility of the method in molecular biology laboratories. We will then carry out extensive field tests of the paired-tag kit in the laboratories of current brain initiative investigators, and further improve IT based on the feedbacks. Finally, we will adapt the paired-tag procedure to long-read sequencing technologies to enable high throughput detection of splicing isoforms in brain cells at single cell resolution. If successful, the proposed research would provide researchers with a powerful new tool for single cell transcriptomics and epigenomics analysis.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $0 | |
| Amendment | $444,953 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | $0 | |
| Amendment | -$60,000 | |
| Amendment | $60,000 | |
| Amendment | $60,000 | |
| Amendment | -$60,000 | |
| Amendment | $60,000 | |
| Amendment | $450,000 | |
| Original award | $450,000 |
Show all 13
Quick answers
Who received award R41MH128993?
R41MH128993 was awarded to Epigenome Technologies, Inc. by National Institute of Mental Health (National Institutes of Health) on September 1, 2022.
How much is award R41MH128993 worth?
$1,404,953 has been obligated on R41MH128993.
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