High-throughput single cell CO-assay of histone modifications and transcriptome
Ended Project Grant Awarded Last action
- Award ID
- R41GM146330
- Recipient
-
Epigenome Technologies, Inc. UEI LS7JQN77JKQ1
- Awarding agency
-
National Institute of General Medical Sciences
National Institutes of Health, Department of Health and Human Services
- Obligated
- $1,050,000
Award facts
- Assistance listing
- 93.859 Biomedical Research and Research Training
- Completion date
- Funding
-
- $700,000.00 Federal
- $350,000.00 Non-federal
- Place of performance
- California, USA
Award details come from USAspending.gov.
Description
- Abstract
- Epigenome analysis can help dissect the transcriptional regulatory sequences that control spatiotemporal patterns of gene expression during anima development and disease pathogenesis, but a major challenge to epigenome analysis is the heterogeneity of primary tissues. Conventional epigenome assays that take bulk tissues as input only produce population average signals. To address this major bottleneck, we propose to develop an ultra-high throughput single-cell multi-omics method, paired-tag, for joint profiling of histone modifications and transcriptome. In preliminary experiments, we have demonstrated the feasibility and utility of this method through analysis of the nuclear transcriptome and multiple histone modifications at single cell resolution in the adult mouse frontal cortex and hippocampus. In the proposed study, we will further optimize the current paired-tag protocol and demonstrate its utility in cancer epigenome analysis. If successful, the research would add a major toolkit for the production of cell-type-resolved maps of chromatin state and transcriptome in complex tissues and enable next generation epigenome analysis of tumor samples.
Transactions
| Action | Federal | Non-federal | Date |
|---|---|---|---|
| Amendment | $0 | $0 | |
| Amendment | $350,000 | $0 | |
| Original award | $350,000 | $350,000 |
Quick answers
Who received award R41GM146330?
R41GM146330 was awarded to Epigenome Technologies, Inc. by National Institute of General Medical Sciences (National Institutes of Health) on September 15, 2021.
How much is award R41GM146330 worth?
$1,050,000 has been obligated on R41GM146330: $700,000 in federal funding and $350,000 in non-federal funding.
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