Project Grant R01HG004819

This Project Grant award of $194,375 from the National Institutes of Health (NIH) Office of Research Infrastructure Programs (CFDA 93.351) supports the University of Wisconsin-Madison in developing robust genome editing tools for generating floxed alleles and editing amino acid changes in the zebrafish model system. The key objectives are to establish a synthetic exon-mediated integration approach to reliably add LoxP sites to target genes, enabling conditional knockout of genes in specific cell...

The National Science Foundation's Division of Integrative Organismal Systems awarded $1,526,917 to Stanford University on September 15, 2025, under the Biological Sciences (CFDA 47.074) program to develop genome editing tools and genomic resources for poeciliid fishes (guppies, mollies, and swordtails). The project will establish immortalized cell lines from multiple species, optimize CRISPR-based DNA editing protocols for both in vitro and in vivo applications, and generate high-quality,...

This Project Grant from the National Science Foundation's Integrative Activities program ($749,255) supports the development of genomic and artificial intelligence tools to address fishery management challenges from harvest to market. Led by Michigan State University from October 2021 to September 2022, the award aims to design CRISPR-Cas9 SHERLOCK assays enabling rapid, inexpensive species identification without specialized expertise. Initial work focuses on scalloped and Carolina hammerhead...

This Project Grant award from the National Human Genome Research Institute (NHGRI), under the Human Genome Research federal grant program (CFDA 93.172), provides $757,157 to the University of California, San Diego (UC San Diego) to develop complementary experimental and computational strategies for high-throughput characterization of the impact of genetic variants. The key objectives are to: 1) Develop a method called SCBE-SEQ that combines genome editing with single-cell sequencing to...

The National Science Foundation (NSF) Division of Environmental Biology awarded a $569,812 Project Grant to the University of Missouri System to study the genetic mechanisms underlying repeated evolution of adaptations to extreme cave environments in certain species of fish. The research will employ cutting-edge sequencing and comparative genomics techniques to identify genetic variations associated with cave-adapted traits in three species of cavefish and their surface relatives. Functional...

This three-year, $926,260 National Science Foundation project grant supports research investigating the genetic and evolutionary factors behind the loss of sperm flagella in weakly electric mormyrid fishes. Funded through the NSF's Biological Sciences program (CFDA 47.074), the award to Michigan State University will determine whether a partial gene deletion results in flagellar loss using CRISPR/Cas9 gene editing in zebrafish. It will also examine sperm-egg interaction and test hypotheses...

This Project Grant award from the National Science Foundation's (NSF) Division of Environmental Biology, under CFDA Program 47.074 Biological Sciences, provides $879,124 to Lehigh University in Bethlehem, Pennsylvania to conduct collaborative research on the genetic mechanisms underlying repeated evolution in species that have adapted to extreme cave environments. The research will leverage comparative genomic analyses and functional genomics techniques like CRISPR-Cas9 to identify common...

The Regents of the University of California, Berkeley has been awarded a $632,806 Project Grant from the National Science Foundation's (NSF) Biological Sciences (CFDA 47.074) program to conduct research on the diversification of retinal ganglion cells in the zebrafish neural retina. This 5-year project will use single-nucleus RNA sequencing, genome engineering, and live imaging techniques to map the transcriptional landscapes and lineage relationships among the approximately 35-50 distinct types...

This federal Project Grant award of $432,357 from the National Science Foundation's (NSF) Biological Sciences program (CFDA 47.074) supports research to understand how fishes have adapted to high-pressure environments in the deep ocean. The research aims to: Characterize patterns of genome evolution across 100 species of perciform fishes found at depths ranging from 0-8,000 meters. Measure structural changes in key biomolecules involved in metabolism, cell movement, and stress responses across...

This Project Grant award from the National Institute of Dental and Craniofacial Research (NIDCR), under the Oral Diseases and Disorders Research program (CFDA 93.121), will support research to establish a novel zebrafish model of syndromic craniosynostosis. The $313,000 award to Emory University will enable comprehensive phenotypic, mechanistic, and genetic analyses of the craniosynostosis mutant zebrafish to better understand the underlying pathological mechanisms. The research aims to identify...