Septhohippocamal connectome dysfunction in down syndrome associated with alzheimer's disease pathophysiology
Active Project Grant Awarded Last action
- Award ID
- R01AG077103
- Recipient
-
New York University UEI M5SZJ6VHUHN8
- Awarding agency
-
National Institute on Aging
National Institutes of Health, Department of Health and Human Services
- Obligated
- $1,530,301
Award facts
- Assistance listing
- 93.310 Trans-NIH Research Support
- Completion date 75% complete
- Funding agency
- Office of the Director National Institutes of Health, Department of Health and Human Services
- Place of performance
- Orangeburg, NY 10962, USA
Award details come from USAspending.gov.
Description
- Abstract
- Down syndrome (DS) is caused by triplication of human chromosome 21 and results in physical and cognitive developmental delay and disability. Individuals with DS transition to alzheimer's disease (ad) in early midlife and develop premature dementia along with histopathological hallmarks of ad including amyloid-beta and TAU pathology, synaptic deficits, and neurodegeneration. This sequence of pathological hits preferentially impacts vulnerable neural networks such as the septohippocampal and basocortical circuits which support attention, memory, and executive function. While DS and ad phenotypes overlap in many respects, the extent of shared cellular pathophysiological mechanisms remains poorly understood. The knowledge GAP is a potentially missed opportunity to arrest the onset of ad dementia in DS. We propose to identify molecular, cellular, and physiological substrates underlying vulnerability of the septohippocampal and basocortical connectomes in trisomic (TS65DN) mice, which faithfully reproduce circuits with memory and executive function deficits in human DS and ad. In parallel, we will study human induced neurons (HIN) derived directly from DS, ad, and control fibroblasts to reveal functional consequences of transcript-level alterations in human neurons. Our cell and animal model findings will be validated in postmortem human brain. Specifically, we propose to identify molecular and cellular substrates underlying calcium signaling and mitochondrial network dynamics within the septohippocampal and basocortical connectomes in young {~4 months of age (MO), middle age (~12 MO) and older (~18 MO)} TS65DN mice relative to normal disomic (2N) littermates and in HIN from DS, ad, and age- matched controls. We will evaluate physiological and synaptic signaling properties of septohippocampal and basocortical neurons in acute mouse brain slices and HIN. We will compare expression profiles from these models to neurons obtained postmortem from individuals with DS, ad, and controls. In aim 1 we will test the hypothesis gene expression pathways regulating calcium handling, oxidative phosphorylation, and synaptic signaling within basal forebrain cholinergic neurons (bfcns) precede defects in hippocampal and frontal cortical pyramidal neurons in trisomic mice. In aim 2 we will test the hypothesis differential gene expression pathways in DS manifest as progressive defects in synaptic and calcium signaling, mitochondrial dysfunction, and protein mishandling in DS cell and animal models. In aim 3 we will test the hypothesis dysregulated genes and pathways in trisomic mice are significantly altered within HIN and analogous postmortem neuronal populations in individuals with DS and ad. This multidisciplinary approach combining single population RNA- sequencing with electrophysiological interrogation enables a determination of the pathobiology underlying bfcn, CA1, and cortical neuron vulnerability in vivo in TS65DN and 2N littermates compared to HIN and postmortem human DS neurons with CO-occurring ad pathology. We posit these previously unavailable findings will generate new therapeutic strategy approaches for DS and ad.
Transactions
| Action | Obligated | Date |
|---|---|---|
| Amendment | $80,543 | |
| Amendment | $724,879 | |
| Original award | $724,879 |
Subawards
| Subrecipient | Amount | Awarded |
|---|---|---|
| Research Foundation For Mental Hygiene, Inc. | $2,234,988 | |
| Rosalind Franklin University Of Medicine & Science | $576,884 |
Quick answers
Who received award R01AG077103?
R01AG077103 was awarded to New York University by National Institute on Aging (National Institutes of Health) on February 15, 2023.
How much is award R01AG077103 worth?
$1,530,301 has been obligated on R01AG077103.
Which agency awarded R01AG077103?
National Institute on Aging (National Institutes of Health) awarded R01AG077103, with funding from Office of the Director (National Institutes of Health).
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