The role of the RPE in neurogenesis and cell fate in retinal ganglion cells
Active Project Grant Awarded Last action
- Award ID
- F32EY038564
- Recipient
-
The Trustees Of Columbia University In The City Of New York UEI QHF5ZZ114M72
- Awarding agency
-
National Eye Institute
National Institutes of Health, Department of Health and Human Services
- Obligated
- $79,348
Award facts
- Assistance listing
- 93.867 Vision Research
- Completion date 0% complete
- Place of performance
- New York, NY 10027, USA
Award details come from USAspending.gov.
Description
- Abstract
- For effective binocular vision, retinal ganglion cells (rgcs) must send projections both ipsilaterally and contralaterally to the brain. If the balance of ipsi- to contralateral rgcs is altered, vision can be impaired. In albino humans and mice, the retina has a reduced number of ipsilateral rgcs, resulting in poor depth perception. In the albino, the retinal pigment epithelium (RPE) lacks melanin, though how this affects the projection fate of rgcs is unknown. In the work proposed here, i will establish the critical molecular differences between the pigmented and albino embryonic RPE and establish the mechanisms underlying the influence of the RPE on RGC cell fate. In aim 1, i will use a new CO-culture system i am developing in order to determine the overall effects of pigmented versus albino RPE on the developing retina, and importantly, if the genotype of the RPE can rescue or inhibit retinal development when the retinal tissue has the opposite genotype. In aim 2, i will investigate mitochondrial properties in the albino RPE, as bulk RNA sequencing results have shown an increase in transcription of many genes associated with the electron transport chain and mitochondrial homeostasis. In aim 3, i will investigate two distinct but related aspects of RPE-retina communication: calcium signaling (aim 3A) and GAP junctions (aim 3B). The research proposed here will advance my training in a new system, the retina and the eye itself, as well as introduce me to new computational methods. I will also develop expertise in approaching study of disorders of the visual system combining mouse models, molecular and cellular approaches, and gene discovery to establish requirements for cell-cell interactions that ultimately determine wiring between eye and brain. My sponsor, DR. Carol mason, is an expert in visual system development and will mentor me in my research and in developing my writing and scientific communication skills. I will complete this research in the zuckerman institute at columbia university, which contains not only world-class facilities and equipment, and programs for postdoc professional development and career navigation, but also houses a superb network of neuroscience researchers and will thus be the ideal environment to perform this research.
Quick answers
Who received award F32EY038564?
F32EY038564 was awarded to The Trustees Of Columbia University In The City Of New York by National Eye Institute (National Institutes of Health) on February 17, 2027.
How much is award F32EY038564 worth?
$79,348 has been obligated on F32EY038564.
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