Awards issued prior to january 20, 2025, were funded under previous administrations and may not reflect the priorities and policies of the current administration.** the proposed aqpathdx kit enables…

Awards issued prior to january 20, 2025, were funded under previous administrations and may not reflect the priorities and policies of the current administration.** the proposed aqpathdx kit enables rapid, pondside detection of the most common catfish and tilapia pathogens in two separate panels in less than 30 minutes using a novel multiplex molecular diagnostic assay. The aqpathdx kit is based on multiplex loop-mediated isothermal amplification (MLAMP) method, which allows real-time detection of up to 4 targets in a single reaction. This assay will be performed on a benchtop isothermal instrument, and the results will be displayed on the screen as positive, negative, or non-conclusive.aqpathdx kit performance will be compared with that of the reference method. A simple and rapid sample preparation method will also be developed, and complete reaction formulation will be lyophilized for ambient storage. The analytical performance of the aqpathdx kit will be evaluated by testing a cohort of 300 positive and 200 negative samples.