The USDA ARS ncccwa requires total RNA extraction and total RNA-SEQ library preparation (poly-a selection) and mrna sequencing of 90 frozen samples of rainbow trout whole-body tissue homogenized in…

Active Purchase Order Awarded Last action

Contract number
1232SA26P0539
Awardee
Awarding agency
Obligated
$15,020

Award facts

Opportunity
Contract value 100% obligated
$15,020.00 Current and potential, with all options
Completion date 47% complete
Current and potential, with all options
Contracting staff
Funding agency
Northeast Area Field Research Implementation and Information Delivery, Department of Agriculture
Set-aside
Total Small Business
Competition
Competed under SAP · 13 offers Simplified Acquisition
Pricing
Firm Fixed Price
Place of performance
Kearneysville, WV 25430, USA

Award details come from SAM.gov/FPDS.

About this award

The U.S. Department of Agriculture Agricultural Research Service awarded a purchase order to Genohub, Inc., a small business, for $15,020.00 on August 24, 2026, to perform total RNA extraction and RNA-seq library preparation with mRNA sequencing of 90 frozen rainbow trout whole-body tissue samples homogenized in Trizol, plus RNA-seq library preparation (poly-A selection) and sequencing of an additional 126 samples (90 spleen tissue and 36 primary muscle cell culture samples). Work is performed at Kearneysville, West Virginia. Sequencing must use Illumina technology with paired-end reads of 2×150 nucleotides, minimum 40 million paired-end reads per sample, and greater than 85 percent of bases at QC30 quality. Extracted RNA must achieve minimum concentration greater than 50 ng/µL, minimum volume greater than 100 microliter, A260/A280 ratio between 1.8 and 2.0, and RIN value greater than 7. All samples will be provided frozen on dry ice within 90 days of contract award. Sequencing data and a Final Project Report are due within 60 days of receipt of all samples. Period of performance runs August 31, 2026 through December 30, 2026.

Description

The USDA ARS ncccwa requires total RNA extraction and total RNA-SEQ library preparation (poly-a selection) and mrna sequencing of 90 frozen samples of rainbow trout whole-body tissue homogenized in trizol, and total RNA-SEQ library preparation (poly

Quick answers

Who was awarded contract 1232SA26P0539?

1232SA26P0539 was awarded to Genohub, Inc. by Central Program Planning Coordination and Support (Agricultural Research Service) on August 24, 2026.

How much is contract 1232SA26P0539 worth?

The government has obligated $15,020 on 1232SA26P0539.

Which agency awarded contract 1232SA26P0539?

Central Program Planning Coordination and Support (Agricultural Research Service) awarded 1232SA26P0539, with funding from Northeast Area (Field Research Implementation and Information Delivery).

Was contract 1232SA26P0539 competed?

The extent of competition for 1232SA26P0539 was competed under sap. The government received 13 offers. It was set aside: Total Small Business.

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