For supplies and related services for Cryo-EM (Kachar, 26-001128)
Past New Requirement Updated
- Agency
-
National Institutes of Health
Department of Health and Human Services
- Solicitation expected
- Estimated value
- $25,000 to $250,000
- Set-aside
- No set-aside
Forecast facts
- Anticipated award
- NAICS code
- 541380 Testing Laboratories and Services
- Points of contact
Forecast details come from HHS's procurement forecast. Agencies' dates and values are estimates.
About this forecast
The National Institutes of Health forecasts a new requirement for supplies and related services supporting cryo-electron microscopy, with solicitation release and award both estimated for June 1, 2026. The work investigates model cell structures at UVA through in situ cryo-electron tomography using Correlative Light and Electron Microscopy, with cryogenic lamella preparation on the Molecular Electron Microscopy Core's Aquilos 2+ cryo-FIB-SEM and integrated fluorescence microscope, followed by high-throughput data collection on Glacios and Krios systems. Data collection uses ThermoFisher Scientific Tomography 5 software and SerialEM/SPACETomo, while AreTomo3 running on a dedicated 4-GPU workstation generates preprocessing and tomographic reconstructions on the fly.
The estimated award value range is $25,000 to $250,000 under NAICS 541380, Testing Laboratories and Services, and no set-aside is used. Work is described as performed at UVA. Points of contact are Brian Lind, Supervisory Program Specialist in NIH Legal-Contracts (brian.lind@nih.gov, +13015948939), and Shiqiong Hu (shiqiong.hu@nih.gov).
Forecast text
To investigate the model cell structures using cryo-electron microscopy at UVA. In situ cryo-ET using Correlative Light and Electron Microscopy (CLEM). The Molecular Electron Microscopy Core's Aquilos 2+ cryo-FIB-SEM, featuring an integrated fluorescence microscope (iFLM), will be utilized to prepare thin cryogenic sections (lamellae) of cellular samples for in situ cryo-ET. Regions of interest (ROIs) will be identified using the iFLM; lamellae will then be prepared at these sites, with post-milling fluorescence imaging used to target specific loci for data acquisition. High-throughput cryo-ET data collection will be performed on a Glacios (equipped with a Falcon 4
detector) and a Krios (equipped with a K3 detector and BioQuantum energy filter) using the latest ThermoFisher Scientific Tomography 5 software and SerialEM/SPACETomo. Initial preprocessing and tomographic reconstructions will be generated on-the-fly using AreTomo3 software running on a dedicated 4-GPU workstation.
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