Synthesis of Self-Amplifying Nucleic Acid Constructs

The Department of Agriculture Agricultural Research Service is seeking the synthesis of five lipid nanoparticle-encapsulated self-amplifying RNA constructs based on Venezuelan equine encephalitis virus (VEEV) genetic components through this Request for Quotation (RFQ). The service requires comprehensive genetic engineering work, including codon optimization for avian expression, gene synthesis, plasmid cloning, in vitro transcription, and RNA encapsulation into SM10 lipid nanoparticles. Offerors must demonstrate technical capability and experience through a detailed technical package and must hold prices firm for 90 calendar days. Award will be made on a lowest price technically acceptable basis to a single contractor. The deadline for submitting questions is January 5, 2026, at 4:00 PM Central Time, with quotations due by February 6, 2026, and all items required to be delivered within 30 days after award.

This acquisition is designated as a Total Small Business Set-Aside, with a small business size standard of $19.0 million, meaning only small business concerns are eligible for award. The contract specifies delivery of one 1 mL aliquot of 250 μg encapsulated RNA for genes 1-4 and two 1 mL aliquots of 250 μg encapsulated RNA for gene 5, with minimum RNA yield requirements of 500 μg for genes 1-4 and 1000 μg for gene 5. Five distinct gene constructs ranging from 828 to 3,525 nucleotides must be developed with comprehensive quality control assessments including encapsulation efficiency, particle size, polydispersity index, zeta potential, endotoxin presence, and pH analysis. The place of performance is the US National Poultry Research Center in Athens, Georgia, with a firm-fixed price contract structure and no incumbent contractor information provided. All offerors must maintain active SAM.gov registration and comply with applicable federal acquisition regulations and provisions incorporated by reference.