Requirements Contract for 454GS (Genome Sequencer) Junior and FLX Consumables

The United States Army Medical Research Acquisition Activity (USAMRAA) has a requirement for a Request for Quotation (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular (FAC) 2001-27.
This is an Unrestricted Competition. The North American Industry Classification System (NAICS) code is 325413.

This requirement will be for a Requirements Indefinite Delivery Indefinite Quantity contract (IDIQ). The Period of Performance for this contract will be a base year with four options years.

Quantities and prices are annual estimates, are dependent on actual funding requirements, and the government is not obligated to purchase.
Under this contract, the customer places order as required, and the vendor delivers as early as the next business day but no later than 5 business days.
Vendor shall provide products with shelf life no shorter than six months from the date of the delivery.
General: The following consumables might be required for the GS Junior System or the GS FLX System currently in use at USAMRIID. Several items will be regularly ordered, dependent on available funding, including the library preparation, emPCR, Titanium sequencing, and Picotiter plate kits as well as maintenance kits. The remaining items could be ordered, dependent on mission requirements, but not all items would be purchased.

GS Junior

1. Control bead kit
Must work as a positive control during a sequencing run on a 454 GS Junior instrument
Must verify that the GS Junior System is performing to manufacturer specifications

Rapid or General Library Preparation Kit
Must contain reagents required to process a single DNA sample into a DNA library of single-stranded DNA fragments compatible with subsequent emulsion amplification and sequencing on a GS Junior System
Must contain all reagents and components required for 12 individual Rapid library preparations or 10 General library preparations.
2. emPCR Kit (Lib-L or Lib-A; 1, 8, or 16 emulsions)
Must contain reagents and vacuum components sufficient for emulsion-based clonal amplification of a DNA library prepared using an appropriate GS Junior Titanium series library preparation procedure (e.g., shotgun, Paired End, cDNA)
Must be compatible with subsequent sequencing on a GS Junior System, using the GS Junior Titanium Sequencing and PicoTiterPlate Kits.

Titanium Paired End Adaptor Kit
Must contain reagents for preparation of 10 paired-end libraries

GS Nebulizer Kit
Must contain reagents for fragmenting 10 library preparations

3. Titanium Sequencing Kit
Must contain reagents and supplies required to determine the nucleotide sequence of a PicoTiter Plate-immobilized and clonally amplified DNA library prepared using the GS Junior System methods
Must be compatible with the 454 GS Junior Titanium PicoTiterPlate Kit and GS Junior System.

4. Picotiter plate kit
Must be compatible with the 454 GS Junior System
Must contain accessories sufficient for one sequencing run on a GS Junior System.

Rapid Library MID Adaptors Kit
Must be compatible with the preparation of multiplexed/indexed DNA libraries for sequencing on the GS Junior System
Must contain twelve 454 adaptors (numbered GS1-GS12) that can be substituted for the adaptors of the GS Rapid Library Preparation Kit.
5. Sipper Maintenance kit
Must contain ten sipper tubes and filters for required monthly maintenance of the GS Junior System.

6. Maintenance wash kit
Must contain reagents and supplies required for monthly maintenance wash runs of the GS Junior System.

GS Titanium emPCR Breaking Kits
Must contain reagents for breaking the emulsions from 12 separate emulsion PCR preparations.

Additional accessories specific for the GS Junior
Must have additional accessories available for the GS Junior including nebulizer holders, IKA Turrax, bead deposition devices for the PicoTiter plates, BDD Counterweights, emPCR bead counter and shaker adaptors, as well as software for running the GS Junior platform.
GS FLX
7. Control bead kit
Must work as a positive control during a sequencing run on a 454 GS FLX instrument
Must verify that the GS FLX System is performing to manufacturer specifications

Rapid or General Library Preparation Kit
Must contain reagents required to process a single DNA sample into a DNA library of single-stranded DNA fragments compatible with subsequent emulsion amplification (using one of the GS FLX Titanium series emPCR kits) and sequencing on a GS FLX System
Must contain all reagents and components required for 12 individual Rapid library preparations or 10 General library preparations.
8. emPCR Kit (Lib-L or Lib-A; SV, MV, LV emulsions; paired end, amplicons)
Must contain reagents and vacuum components sufficient for emulsion-based clonal amplification of a DNA library prepared using an appropriate GS FLX Titanium series library preparation procedure (e.g., shotgun, Paired End, cDNA, amplicons)
Must be compatible with subsequent sequencing on a GS FLX System, using the GS FLX Titanium Sequencing and PicoTiterPlate Kits.

Titanium Paired End Adaptor Kit
Must contain reagents for preparation of 10 paired-end libraries

GS Nebulizer Kit
Must contain reagents for fragmenting 10 library preparations

9. Titanium Sequencing Kit
Must contain reagents and supplies required to determine the nucleotide sequence of a PicoTiter Plate-immobilized and clonally amplified DNA library prepared using the GS FLX System methods
Must be compatible with the 454 GS FLX Titanium PicoTiterPlate Kit and GS FLX System.

10. Picotiter plate kit
Must be compatible with the 454 GS FLX System.

Rapid Library MID Adaptors Kit
Must be compatible with the preparation of multiplexed/indexed DNA libraries for sequencing on the GS FLX System
Must contain twelve 454 adaptors (numbered GS1-GS12) that can be substituted for the adaptors of the GS Rapid Library Preparation Kit.
11. Maintenance wash kit
Must contain reagents and supplies required for monthly maintenance wash runs of the GS FLX System.

GS Titanium emPCR Breaking Kits
Must contain reagents for breaking the emulsions from 12 separate emulsion PCR preparations.

This award is for the consumable kits on an as-needed basis.

All responsible sources may submit a quote, which shall be considered by the agency. The competitive solicitation, solicitation amendments and all questions and answers relating to this procurement shall be made available via the Internet at https://acquisition.army.mil/asfi/ or https://www.fedbizopps.gov.

The solicitation is anticipated for release to the vendor community on or about, July 13, 2011, 2011 and will only be available until August 15, 2011. Specifications of the requirement are provided in solicitation: W81XWH-11-T-0455.

Potential offerors are responsible for accessing the website. Interested parties must respond to the solicitation in order to be considered for award of any resultant contract. To be eligible for an award, offerors MUST be registered with the Central Contractor Registry at https://ccr.gov/ and may have completed its representations and certification found at https://orca.bpn.gov/.

All questions should be addressed in writing to Colleen Barnes, Contract Specialist at colleen.barnes@us.army.mil. Questions shall be submitted not later than 1200 hours (Eastern Day Light Savings Time) on August 2, 2011. Only one set of questions shall be received from each offeror. Numerous emails from one offeror shall not be accepted. Telephone requests will not be honored and bidder lists will not be maintained.

All interested parties shall provide their quote to Colleen Barnes at colleen.barnes@us.army.mil by 10:00 a.m. ET on August 15, 2011.