Reagents and Consumables

This notice announces the intent by the U.S. Army Medical Research Acquisition Activity (USAMRAA) in support of the Armed Forces Medical Examiner System AFMES-DoD DNA Operations (AFMES-DNAOPS) section to procure customized Reagents and Consumables manufactured by ThermoFisher/Life Technologies Brand. AFMES-DNAOPS, is DoD’s only human remains DNA testing laboratory, is responsible for performing all DNA testing for the identification of fallen service members from current day and past conflicts. These reagents and supplies have been designed, previously optimized, and validated specifically for forensic laboratories, including equipment-specific for the various procedures of DNA identification. The reagents have been tested and are compatible for use with: Life Technologies Genetic Analyzers Model 3500xl.

The following reagents will be required:

96-Capillary Array Cartridge 50 um (ID) 33 cm (EFF), 55 cm (TOT), 10mL (RES)

Part # A2300-9650-3355

• For use on the Fragment Analyzer system
• Shall include 96 Capillaries
• Shall be replaceable
• Shall contain capillaries of  33 cm (EFF) in length or longer
• Shall contain capillaries with 50um (Inner Diameter) or larger

48-Capillary Array Cartridge 50 um (ID) 33 cm (EFF), 55 cm (TOT), 10mL (RES)

Part # A2300-4850-3355

• For use on the Fragment Analyzer system
• Shall include 48 Capillaries
• Shall be replaceable
• Shall contain capillaries of  33 cm (EFF) in length or longer
• Shall contain capillaries with 50um (Inner Diameter) or larger

Capillary Storage Solution, 100 ml Bottle

Part # GP-440-0100

• For use on the Fragment Analyzer system
• A proprietary mixture of Glycerine (CAS: 56-81-5) required to keep capillaries on a Fragment Analyzer properly humidified between runs

Gasket Kit

Part# G2938-68716

• Shall include 1 syringe adapter
• Shall include 10 gaskets
• Shall include 1 mounting ring
• Shall be compatible with Bioanalyzer Chip Priming Station

Chip Priming Station

Part # 5065-4401

• For use with the Bioanalyzer quantification system
• Shall include priming station, stopwatch and 1 syringe clip
• Shall be compatible with Agilent DNA and RNA assay chips

dsDNA Reagent Kit, 75-15,000 bp (500 samples)

Part # DNF-920-K0500

• For use on the Fragment Analyzer system
• Provides sizing and qualitative analysis of PCR fragments up to 15,000bp
• Sizing Range: 75 bp – 15,000 bp (defined by lower/upper marker)
• Input Concentration Range: 0.5 ng/μL – 50 ng/μL

High Sensitivity NGS Fragment Analysis Kit (1 bp - 6000 bp), (500 samples)

Part # DNF-474-0500

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis of NGS libraries
• Sizing Range: 25 bp – 5,000 bp
• Input Concentration Range: 50 pg/μL – 5 ng/μL

Standard Sensitivity NGS Fragment Analysis Kit (1 bp - 6000 bp), (500 samples)

Part # DNF-473-0500

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis of NGS libraries
• Sizing Range: 25 bp – 5,000 bp

Input Concentration Range: 5 ng/μL – 100 ng/μL

Large NGS Fragment Analysis Kit, (500 samples)

Part # DNF-492-0500

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis of NGS libraries with large fragments
• Sizing Range: 50 bp – 20,000 bp
• Input Concentration Range: 5 ng/μL – 100 ng/μL

High Sensitivity Large Fragment Analysis Kit, (500 or more samples)

Part # DNF-464-0500

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis of NGS libraries with large fragments
• Sizing Range: 75 bp – 48,500 bp
• Input Concentration Range: 50 pg/μL – 5 ng/μL

dsDNA 935 Reagent Kit, 1-1,500 bp (500 samples)

Part # DNF-935-K0500

• For use on the Fragment Analyzer system
• Provides sizing and qualitative analysis of PCR fragments up to 1,500bp
• Allows for fast separation in under 20 minutes
• Sizing Range: 1 bp – 1,500 bp (defined by lower/upper marker)
• Input Concentration Range: 0.5 ng/μL – 50 ng/μL

High Sensitivity Large NGS Fragment Analysis Kit, (500 samples)

Part# DNF-488-0500

• For use on the Bioanalyzer system
• Provides sizing and quantitative analysis of NGS libraries with large fragments
• Sizing Range: 100 bp – 7500 bp
• Input Concentration Range: 50 pg/μL – 5 ng/μL

Agilent DNA 7500 Kit, (300 samples/25 chips)

Part# 5067-1506

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis dsDNA
• Sizing Range: 75 bp – 20,000 bp
• Input Concentration Range: 0.5 ng/μL – 50 ng/μL

Agilent DNA High Sensitivity DNA Kit, (110 samples/10 chips)

Part# 5067-4626

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis dsDNA
• Sizing Range: 50 bp – 7,000 bp

Agilent DNA 12000 Kit, (300 samples/25 chips)

Part# 5067-1508

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis dsDNA
• Sizing Range: 100 bp – 12,000 bp
• Input Concentration Range: 0.5 ng/μL – 50 ng/μL

Agilent Protein 230 Kit, (250 samples/25 chips)

Part# 5067-1517

• For use on the Fragment Analyzer system
• Provides sizing and quantitative analysis dsDNA
• Quantitative Range: 15-2000 ng/μL

Herculase II Fusion Polymerase (200rxn)

Part# 600677

• Shall be formulated to amplify difficult GC rich targets
• Shall include dNTPs

PfuTurbo Cx Hotstart DNA Polymerase

Part # 600414

• Shall be a mutant Pfu polymerase designed to overcome Uracil sensitivity

The authority permitting other than full and open competition is the 10 U.S.C. 2304(c)(1) and the FAR Part 6.302-1, Only One Responsible Source and no other supplies or services will satisfy agency requirements. ThermoFisher/Life Technologies is the sole manufacturer of the systems, kits, reagents, and consumables listed above. These reagents and consumables were specifically developed by ThermoFisher/Life Technologies for DNA forensic testing and validated by AFMES-DNAOPS to work in conjunction with AFMES-DNAOPS’s current analysis instrumentation and accredited standard operating procedures.

The North American Industry Classification System (NAICS) for this requirement is 334516 and PSC 6515.

This Special Notice of Intent is not a request for competitive quotes. Information received in response to this notice will be considered solely for the purpose of determining whether a competitive procurement is in the best interest of the Government.

All questions and responses concerning this notice shall be emailed to Brian Miller at brian.d.miller1.civ@health.mil.