Production of ten adenovirus type 5 (AD5) viruses expressing pasmodium yoelii antigens.

The United States Department Of Naval Medical Research Center(NMRC) intends to award a sole source contract, DO 260 Inc, Boise ID 83701 This notice is not a request for competitive proposals however If interested Government shall consider all responses.

1. Step 2.2.1 Shuttle plasmid construction - 10 EA.
2. Step 2.2.2 Adenovirus cosmid construction - 10 EA.
3. Step 2.2.3 and Step 2.2.3 Virus rescue and virus identity and stabilility - 10 EA.
4. Step 2.4.1 and Step 2.4.2 and Step 2.5.3 Large-scale virus amplication and purification - 10 EA.
5. Step 2.4.3 and Step 2.5.5 Infectious titer determination (tcid50) and Particle/IU ratio - 10 EA.
6. Step 2.5.1 Sequencing of traqnsgene in purified virus - 10 EA
7. Step 2.5.2 Transgene expression level testing - 10 EA.
8. Step 2.5.4 RCA assay - 10 EA
9. Step 2.5.6 In vitro toxicoty - 10 EA
10. Shipping - 1 EA
The NAICS code for this action is 541990. This requirement will be procured under the guidelines of FAR Part 12 Commercial Items. NMRC requires that all contractors doing business with the Acquisition Office must be registered with the Systems For Award Management (SAM). No award can be made unless the vendor is registered in Systems For Award Management. For additional information and to register in SAM, please access the following website: http://www.sam.gov/. In order to register with the SAM and to be eligible to receive an award from this Acquisition Office, all offers must have a DUN & Bradstreet Number. A Dun & Bradstreet number may be acquired free of charge by contacting Dun & Bradstreet online at https://www.dnb.com/products/eupdate/requestOptions.html or by phone at (800) 333-0505

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