High-density single nucleotide polymorphisms (SNP) marker panel
Closed Solicitation Posted
This opportunity was awarded. See the award notice from .
- Solicitation number
- 1232SA26Q0716
- Agency
- Agricultural Research Service Department of Agriculture
- Responses due
- Set-aside
- Total Small Business
Opportunity facts
- NAICS code
- 541380 Testing Laboratories and Services
- Place of performance
- Fort Pierce, Florida 34945, United States
- Points of contact
-
- Kerrie Hodges kerrie.hodges@usda.gov
Notice details come from SAM.gov. Updated .
About this opportunity
The USDA Agricultural Research Service seeks a high-density SNP marker panel for Florida pompano genotyping, with quotations due July 10 and questions due June 26. This is a Total Small Business set-aside under NAICS 541380, limiting competition to small business concerns.
Place of performance is Fort Pierce, Florida. The contractor must perform whole-genome sequencing on 50 fish samples organized into 5 pools of 10 samples each, achieve 20x coverage per individual sample, and develop a fixed-content genotyping panel containing 30,000–60,000 SNP markers using hybrid capture NGS methodology. Samples consist of fin clips (approximately 1–2 cm long, yielding over 30 mg of tissue) preserved in RNAlater cryovials on dry ice; all samples ship in a single batch classified as BSL1. PCR-free library preparation and DNA extraction are required. The panel design must integrate provided low-density marker data, validate existing sex-linked markers when combined with newly generated high-density markers, and nest existing low-density loci while accommodating tiered panel options at consistent cost/density ratios. The reference genome is NCBI GenBank Accession GCA_040938265.2 (contig-level assembly); Hi-C scaffolding is not required. Deliverables include the SNP panel design, raw and filtered data files (FASTQ, variant/genotype calls, FASTA sequences), detailed reproducible methods documentation, and an interpretive report of SNP results. The fully validated panel must be delivered 8 months after award to 2001 South Rock Road, Fort Pierce, FL 34945. The contract is a firm-fixed-price purchase order for one one-year base period (August 1, 2026 to July 31, 2027). Evaluation uses lowest price technically acceptable criteria, with technical acceptability rated as acceptable or unacceptable, and past performance rated as acceptable, neutral, or unacceptable. Award goes to the lowest priced technically acceptable offeror with acceptable or neutral past performance. Questions and answers were updated most recently on June 29.
Notice text
4 versions
Update #4 · Latest ·
***Questions and Answers updated on 6/29/2026 in Attachments***
**Questions and Answers updated on 6/16/2026 in Attachments**
*Questions and Answers from 6/15/2026 in Attachments*
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0716 is issued as a Request for Quotation (RFQ) for high-density single nucleotide polymorphisms (SNP) marker panel service for United Stated Department of Agriculture (USDA) Horticultural Research Lab in Fort Pierce, Florida.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services. The small business size standard is $19M. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency.
Update #3 ·
**Questions and Answers updated on 6/16/2026 in Attachments**
*Questions and Answers from 6/15/2026 in Attachments*
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0716 is issued as a Request for Quotation (RFQ) for high-density single nucleotide polymorphisms (SNP) marker panel service for United Stated Department of Agriculture (USDA) Horticultural Research Lab in Fort Pierce, Florida.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services. The small business size standard is $19M. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency.
Update #2 ·
*Questions and Answers from 6/15/2026 in Attachments*
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0716 is issued as a Request for Quotation (RFQ) for high-density single nucleotide polymorphisms (SNP) marker panel service for United Stated Department of Agriculture (USDA) Horticultural Research Lab in Fort Pierce, Florida.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services. The small business size standard is $19M. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency.
Update #1 ·
This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Part 12. This announcement constitutes the only solicitation. Quotations are being requested and a separate written solicitation will not be issued.
Solicitation number 1232SA26Q0716 is issued as a Request for Quotation (RFQ) for high-density single nucleotide polymorphisms (SNP) marker panel service for United Stated Department of Agriculture (USDA) Horticultural Research Lab in Fort Pierce, Florida.
This acquisition is set-aside for small business concerns. The applicable North American Industry Classification Standard Code is 541380 – Testing Laboratories and Services. The small business size standard is $19M. This acquisition is a Total Small Business Set-Aside. All responsible sources may submit a quotation which will be considered by the agency.
Attachments
| File | Type | Posted |
|---|---|---|
| Questions and Answers 29 June.pdf | ||
| Questions and Answers 16 June.pdf | ||
| Questions and Answers 15 June.pdf | ||
| Solicitation_1232SA26Q0716.pdf |
Notice history
| Notice | Type | Posted |
|---|---|---|
| High-density single nucleotide polymorphisms (SNP) marker panel | Award Notice | |
| High-density single nucleotide polymorphisms (SNP) marker panel | Solicitation |
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