CFX Duet Real-Time PCR System and Accompanying Software

The USDA Agricultural Research Service is requesting quotations for three Bio-Rad CFX Duet Real-Time PCR Systems with 96-well blocks and 2-color detection plus FRET capability, along with one CFX Maestro Software 2.3 license for Windows PCs. The systems must include base units, cables, a minimum one-year warranty from installation completion, and training for new employees as part of the total cost. Key requirements include the ability to read all 96 wells within 12 seconds, achieve 10 orders of magnitude linear dynamic range, detect single copy target sequences, accommodate reaction volumes of 1-50 microliters, and maintain temperature accuracy of 0.2 degrees Celsius at 90 degrees Celsius. The software must support multiplex amplification of up to five fluorophores, multiple standard curves, gene expression analysis, and data export to Excel with optional FDA 21 CFR Part 11 compliance. Award will be made to the lowest-priced technically acceptable offer based on evaluation of price, technical acceptability, and past performance. Quotations must be submitted electronically by May 8, 2026, at 3:00 p.m. Central Time, with questions due by May 4, 2026, at 12:00 p.m. Central Time.

This is an unrestricted acquisition with no set-aside designation, meaning all responsible sources are eligible to submit quotations. No incumbent contractor information is provided in the solicitation documentation. The specific budget or award value has not been disclosed. All items must be delivered to the USDA ARS facility in Baton Rouge, Louisiana by June 30, 2026, with a performance period from May 11, 2026 to June 30, 2026. Vendors must provide firm fixed-price quotations on company letterhead with unit prices, total costs, and their SAM Unique Entity Identifier number, along with a technical proposal demonstrating how proposed products meet the salient characteristics. The procurement is classified under NAICS code 334516 (Analytical Laboratory Instrument Manufacturing) with a small business size standard of 1,000 employees.