Cdiff PCR kits/Smartcycler and Staph SR Assay Kits

The VA Pittsburgh Healthcare System intends to negotiate on a sole source

basis with BD GeneOhm Sciences, 6146 Nancy Ridge Drive, San Diego,

California 92121 for the BD GeneOhm Cdiff Assay kit and the Staph SR

Assay Kit. The BD GeneOhm Cdiff Assay kit is composed of the following

assay specific reagents, and utilizes the Smart Cycler 2 instrument to provide

test results.

Sample buffer Tris-EDTA buffer, Lysis tube Glass beads, Master mix
% DNA polymerase complex,
containing tcdb-primer binding sequences and a unique sequence for probe

hybridization,
Nucleotide mix (dATP, dCTP, dGTP, dTTP) ,Bovine serum albumin,

Carbohydrate, MgCL2,
coli (ATCC 25922) Control DNA Tris-EDTA buffer, Carbohydrate,
non-infectious genomic DNA of C. difficile bearing tcdB gene (ATCC 43255),

Diluent Tris-HCl buffer, MgCL2, (NH4)2SO4, KCl.

The BD GeneOhm Staph SR Assay kit is composed of the following assay

specific reagents, and utilizes the Smart Cycler 2 instrument to provide test

results.

Sample buffer Tris-EDTA buffer, Lysis tube Glass beads, Master mix
% DNA polymerase complex,
containing MRSA-primer binding sequences and a unique sequence for probe

hybridization,
% dATP, dCTP, dGTP, dTTP, Bovine serum albumin, Carbohydrate, MgCL2,


(ATCC 14990) Control DNA Tris-EDTA buffer, Carbohydrate,
genomic MRSA DNA (ATCC 43300), Diluent Tris-HCl buffer,

MgCL2, (NH4)2SO4, KCl.

This Sources Sought Notice is not a request for competitive proposals. It is not a solicitation or request for offers. No solicitation package is available. Vendors who wish to identify their interest and capability to provide Cdiff assay kits and Staph SR assay kits, compatible with the Smart Cycle 2 equipment, may notify the Contracting Officer, in writing, no later than 12:00

P.M. on December 14, 2010. Such notification must include a complete description of proposed assays, including narrative and capabilities described above.