B01.01 W9132T24Q0007 Amendment 0001.pdf

W81EWF33329554

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15A. NAME AND TITLE OF SIGNER (Type or print)

30-105-04EXCEPTION TO SF 30

APPROVED BY OIRM 11-84

STANDARD FORM 30 (Rev. 10-83) Prescribed by GSA

FAR (48 CFR) 53.243

The purpose of this amendment is to make edits to Section C.4 and C.6.

1. CONTRACT ID CODE PAGE OF PAGES

J 1 6

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

16C. DATE SIGNED

BY 30-Jan-2024

16B. UNITED STATES OF AMERICA15C. DATE SIGNED15B. CONTRACTOR/OFFEROR

(Signature of Contracting Officer)(Signature of person authorized to sign)

8. NAME AND ADDRESS OF CONTRACTOR (No., Street , County, State and Zip Code) X W9132T24Q0007

X 9B. DATED (SEE ITEM 11)

24-Jan-2024

10B. DATED (SEE ITEM 13)

9A. AMENDMENT OF SOLICITATION NO.

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

X The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offer is extended, X is not extended.

Offer must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended by one of the following methods:

(a) By completing Items 8 and 15, and returning copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted;

or (c) By separate letter or telegram which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN

REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by telegram or letter, provided each telegram or letter makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

12. ACCOUNTING AND APPROPRIATION DATA (If required)

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE

CONTRACT ORDER NO. IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation date, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(B).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

E. IMPORTANT: Contractor is not, is required to sign this document and return copies to the issuing office.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

10A. MOD. OF CONTRACT/ORDER NO.

2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO.(If applicable)

6. ISSUED BY

3. EFFECTIVE DATE

30-Jan-2024

CODE

ERDC CONTRACTING OFFICE

CHAMPAIGN OFFICE

P O BOX 9005

CHAMPAIGN IL 61826-9005

W9132T 7. ADMINISTERED BY (If other than item 6)

4. REQUISITION/PURCHASE REQ. NO.

CODE

See Item 6

FACILITY CODECODE

EMAIL:TEL:

W9132T24Q0007

SECTION SF 30 BLOCK 14 CONTINUATION PAGE

SUMMARY OF CHANGES

SECTION C - DESCRIPTIONS AND SPECIFICATIONS

The following have been modified:

PERFORMANCE WORK STATEMENT

PERFORMANCE WORK STATEMENT (PWS)

DNA Sequencing and Data Interpretation for Environmental DNA Metabarcoding Libraries

1. INTRODUCTION: The Engineer Research and Development Center – Construction Engineering Research

Laboratory (ERDC-CERL) is leading several projects that utilize molecular tools, specifically environmental DNA (eDNA), to survey for endangered, at-risk and invasive species and whole community biodiversity on military and related lands. ERDC-CERL seeks support for DNA sequencing of DNA metabarcoding samples, to include quality control and data interpretation. ERDC-CERL will be responsible for sample collection and preparation of sequence libraries (adaptors ligated and indexed). The Contractor shall provide consultation for determining optimal sequencing strategy/platform and quality controls for prepared libraries prior to sequencing, perform library quality control, DNA sequencing, and demultiplexing of sequence libraries, and assist with data interpretation and troubleshooting.

2. OBJECTIVE: The objective of this effort is to derive DNA sequence data for a suite of environmental DNA projects that survey for endangered, at-risk and invasive species and whole community biodiversity on military and related lands. The Contractor shall provide consultation for determining optimal sequencing strategy/platform and quality controls for prepared libraries prior to sequencing, perform library quality control, DNA sequencing, and demultiplexing of sequence libraries, and assist with data interpretation and troubleshooting. The resulting data will be incorporated into reporting to installation and DoD sponsors regarding distribution of focal species on military installations.

In reference to DFARS Clauses 252.204-7012 & 252.204-7020 this effort is not considered to include any covered defense information.

3. MAJOR REQUIREMENTS: In order to accomplish the above objective, the Contractor shall complete the following tasks:

a. Task 1. Determination of optimal sequencing strategy and quality controls. The Contractor shall provide consultation for determining optimal sequencing strategies and quality controls needed to meet ERDC-CERL project objectives. Any information pertaining to sequence libraries (e.g., amplicon length, DNA concentration, target loci, indexes, preparation method, sample-size) needed for the Contractor to assist with this task shall be provided by ERDC-CERL. All planning-level sequencing and quality control strategies shall be discussed and decided via e-mail, phone, web conference, and/or in-person meetings between the Contractor and ERDC-CERL. The contractor shall ensure next-day frozen secured shipping of irreplaceable prepared DNA libraries with updates on shipping status, confirmation of samples received, and estimated sequencing timeframe communicated back to ERDC-CERL upon receipt of samples.

b. Task 2. Quality check of prepared sequence libraries. The Contractor shall perform a quality check for each library provided prior to DNA sequencing. Quality checks shall include a fragment analysis and qPCR to validate fragment length distribution and Illumina adaptors, respectively. Should the fragment analysis reveal nontarget bands, the Contractor will assist in a further library cleanup. The Contractor shall provide a report detailing the results of the quality check to ERDC-CERL prior to DNA sequencing before proceeding with sequencing. The Contractor shall provide recommended troubleshooting actions based on any abnormalities identified in quality check report and subsequent changes to sequencing strategies shall be discussed and decided prior to sequencing via e-mail, phone, web conference, and/or in-person meetings between the Contractor and ERDC-CERL.

c. Task 3. DNA sequencing and demultiplexing of validated sequence libraries. After quality check validation (Task 2), the Contractor shall perform DNA sequencing on validated libraries. Eleven independent sequencing runs shall be performed with the Illumina NovaSeq X Plus platform using the 10B flowcell with paired-reads, 2x150 bp reagent chemistry. An additional three independent sequencing runs shall be performed with the Illumina NovaSeq 6000 using the SP flowcell with paired-reads, 2x250 bp reagent chemistry or the equivalent approach on an Illumina NovaSeq X Plus platform with flowcell and chemistry for paired-end sequencing 250 bp fragments. All resulting sequencing reads shall be demultiplexed by the Contractor and provided to ERDC-CERL along with a report detailing sequencing results (general statistics, read counts, quality scores, duplication, read lengths, adaptor content, etc.).

Results for each library should be received within one month of library shipment.

d. Task 4. Data interpretation and troubleshooting solutions for DNA sequence products. The

Contractor shall provide an interpretation and/or potential explanation of any sub-optimal sequence products. Any issues regarding sequence products and potential troubleshooting solutions shall be discussed via e-mail, phone, web conference, and/or in-person meetings between the Contractor and ERDC-CERL and will require additional troubleshooting and sequencing in the event of sequencing error.

4. GOVERNMENT-FURNISHED SUPPORT/INFORMATION/PROPERTY: Over the total period of this award the government will provide a total of fourteen (14) libraries to be sequenced that were constructed for previously collected environmental/biological samples, utilizing the contractor provided shipping methodology and supplies. The exact timing of providing these is unknown but they will not all be provided at the same time.

The government will provide as much advance notice as possible prior to providing each library and will keep the contractor informed of this at the progress teleconference meetings. Government project team members will be available to discuss optimal sequencing and quality control strategies, data interpretation, and troubleshooting.

5. CONTRACTING OFFICER’S REPRESENTATIVE (COR): The ERDC CERL COR for this task order will be appointed in accordance with DFARS 252.201-7000, and a copy of the designation letter will be provided to the Contractor. Although other Government personnel may clarify technical points or supply relevant information to the Contractor regarding this task order, only a Contracting Officer has the authority to revise any requirements in these specifications, including those resulting from verbal clarification. Only a warranted Contracting Officer (either a Procuring Contracting Officer (PCO), or an Administrative Contracting Officer (ACO)), acting within their delegated limits, has the authority to issue modifications or otherwise change the terms and conditions of this contract. If an individual other than the Contracting Officer attempts to make changes to the terms and conditions of this contract, you shall not proceed with the change and shall immediately notify the Contracting Officer.

The Government and the Contractor understand and agree that the services to be delivered under this contract by the Contractor to the Government are non-personal services and the parties recognize and agree that no employer-employee or master-servant relationship exists or will exist under the contract between the Government and the Contractor’s employees.

6. MEETINGS AND REVIEWS: The contractor shall attend the following:

a. A kick off meeting, either in-person, web conference, or via telephone, shall be held no later than 30 working days after award. One objective of this meeting is for the contractor to have their shipping supplies in route to the CERL team to utilize for preparing samples.

b. Speak telephonically with the Engineering Research and Development Center-Construction Engineering Research Laboratory (ERDC-CERL) Contracting Officer’s Representative (COR) as necessary, at mutually agreeable times and dates, to discuss progress to date and anticipated problems, and to ensure that the Contractor's work is compatible with ERDC-CERL work on other aspects of the project. Periodic progress meetings via telephone shall be scheduled, based on sample delivery schedule, throughout the contract performance period.

7. TRAVEL REQUIREMENTS: N/A

8. REPORTS/DELIVERABLES: The Contractor shall submit the following reports/deliverables to the ERDC-

CERL COR, or to the required reporting system specified, within the time specified:

a. Sequencing Data Reports - Reports describing progress on the project, results obtained, and lessons learned shall be submitted to the COR via email within 30 days of completion of DNA sequencing for each sequence library. This report shall contain DNA sequencing and demultiplexing results, data interpretation, and recommended troubleshooting actions if needed. The report shall document all sequencing issues identified and potential explanation of any sub-optimal sequence products.

Invoices for partial payment shall be submitted to coincide with submission of the status reports, and a copy shall be included as an attachment in WAWF (see Section G). No partial payment will be approved unless the government has received all status reports which are due.

11. ANTI-TERRORISM/OPERATIONAL SECURITY:

General security requirements and guidance: The security requirements described below apply to all contract personnel (including employees of the prime Contractor (“Contractor”) and all subcontractor employees) supporting the performance requirements of this contract. The Contractor is responsible for compliance with these security requirements. Questions regarding security matters shall be addressed to the designated Government representative (e.g., Contracting Officer Representative (COR), Requiring Activity (RA) representative, or Contracting Officer (if a COR or other RA representative is not appointed)).

Contract personnel are critical to the overall security and safety of US Army Corps of Engineers (USACE) installations, facilities and activities, and security awareness training contributes to those efforts. The Department of Defense (DoD) and Army security training requirements specified below, if applicable, are performance requirements; all applicable contract personnel shall complete initial training within 30 days of contract award or the date new contract personnel begin performance on the contract. Within five business days from the completion of training, the Contractor shall provide written documentation (e.g., email or memorandum) to the Government representative. The documentation shall include the names of contract personnel trained and which training they completed; the Contractor shall maintain training records as part of their contract files and be prepared to provide copies of training certificates to the Government representative. Contractor personnel and vehicles are subject to search when entering federal installations. Additionally, all contract personnel shall comply with Force Protection Condition (FPCON) measures, Random Antiterrorism Measures (commonly referred to as “RAMs”), and Health Protection Condition (HPCON) measures. The Contractor is responsible for meeting performance requirements during elevated FPCON and/or HPCON levels in accordance with applicable RA plans and procedures --this includes identifying mission essential and non-mission essential personnel. In addition to the changes otherwise authorized by the changes clause of this contract, should the FPCON or HPCON levels at any individual facility or installation change, the Government may implement security changes that affect contract personnel. The Contractor shall ensure all contract personnel are aware of their security responsibilities, including any site-specific requirements identified in local policies or procedures.

12. PERIOD OF SERVICE: All work to be performed shall be completed no later than twelve (12) months after award

Performance Requirements Summary

Deliverable/Event Paragraph Number

Standard Maximum Allowable Degree of Deviation

Method of Surveillance

Remedy for Non- Conformance

Sequencing Data Reports

PWS 8.a. 1)Submitted by required deadline

2) Contains detail of achievements made and problems encountered during the reporting period, as well as a forecast of achievements for the next reporting period.

5% Review by COR Re-performance

(End of Summary of Changes)