W9114F-20-Q-0026-0001 .pdf

W91FVL943121

AMENDMENT OF SOLICITATION/MODIFICATION OF CONTRACT

Except as provided herein, all terms and conditions of the document referenced in Item 9A or 10A, as heretofore changed, remains unchanged and in full force and effect.

15A. NAME AND TITLE OF SIGNER (Type or print)

30-105-04EXCEPTION TO SF 30

APPROVED BY OIRM 11-84

STANDARD FORM 30 (Rev. 10-83) Prescribed by GSA

FAR (48 CFR) 53.243

The purpose of this Amendment is to revise selected Salient Characteristics in FAR Provision 52.212-2.

See Summary of Changes for Details.

1. CONTRACT ID CODE PAGE OF PAGES

J 1 12

16A. NAME AND TITLE OF CONTRACTING OFFICER (Type or print)

16C. DATE SIGNED

BY 18-Mar-2020

16B. UNITED STATES OF AMERICA15C. DATE SIGNED15B. CONTRACTOR/OFFEROR

(Signature of Contracting Officer)(Signature of person authorized to sign)

8. NAME AND ADDRESS OF CONTRACTOR (No., Street, County, State and Zip Code) X W9114F20Q0026

X 9B. DATED (SEE ITEM 11)

06-Mar-2020

10B. DATED (SEE ITEM 13)

9A. AMENDMENT OF SOLICITATION NO.

11. THIS ITEM ONLY APPLIES TO AMENDMENTS OF SOLICITATIONS

X The above numbered solicitation is amended as set forth in Item 14. The hour and date specified for receipt of Offer is extended, X is not extended.

Offer must acknowledge receipt of this amendment prior to the hour and date specified in the solicitation or as amended by one of the following methods:

(a) By completing Items 8 and 15, and returning 1 copies of the amendment; (b) By acknowledging receipt of this amendment on each copy of the offer submitted;

or (c) By separate letter or telegram which includes a reference to the solicitation and amendment numbers. FAILURE OF YOUR ACKNOWLEDGMENT TO BE RECEIVED AT THE PLACE DESIGNATED FOR THE RECEIPT OF OFFERS PRIOR TO THE HOUR AND DATE SPECIFIED MAY RESULT IN

REJECTION OF YOUR OFFER. If by virtue of this amendment you desire to change an offer already submitted, such change may be made by telegram or letter, provided each telegram or letter makes reference to the solicitation and this amendment, and is received prior to the opening hour and date specified.

12. ACCOUNTING AND APPROPRIATION DATA (If required)

13. THIS ITEM APPLIES ONLY TO MODIFICATIONS OF CONTRACTS/ORDERS.

IT MODIFIES THE CONTRACT/ORDER NO. AS DESCRIBED IN ITEM 14.

A. THIS CHANGE ORDER IS ISSUED PURSUANT TO: (Specify authority) THE CHANGES SET FORTH IN ITEM 14 ARE MADE IN THE

CONTRACT ORDER NO. IN ITEM 10A.

B. THE ABOVE NUMBERED CONTRACT/ORDER IS MODIFIED TO REFLECT THE ADMINISTRATIVE CHANGES (such as changes in paying office, appropriation date, etc.) SET FORTH IN ITEM 14, PURSUANT TO THE AUTHORITY OF FAR 43.103(B).

C. THIS SUPPLEMENTAL AGREEMENT IS ENTERED INTO PURSUANT TO AUTHORITY OF:

D. OTHER (Specify type of modification and authority)

E. IMPORTANT: Contractor is not, is required to sign this document and return copies to the issuing office.

14. DESCRIPTION OF AMENDMENT/MODIFICATION (Organized by UCF section headings, including solicitation/contract subject matter where feasible.)

10A. MOD. OF CONTRACT/ORDER NO.

2. AMENDMENT/MODIFICATION NO. 5. PROJECT NO.(If applicable)

6. ISSUED BY

3. EFFECTIVE DATE

18-Mar-2020

CODE

W40M RHCO USAHCA EUROPE

REGIONAL HEALTH CONTR OFC EUROPE

CMR 402

APO AE 09180

W9114F 7. ADMINISTERED BY (If other than item 6)

4. REQUISITION/PURCHASE REQ. NO.

CODE

See Item 6

FACILITY CODECODE

EMAIL:TEL:

W9114F20Q0026

SECTION SF 30 BLOCK 14 CONTINUATION PAGE

SUMMARY OF CHANGES

SECTION SF 1449 - CONTINUATION SHEET

SUPPLIES OR SERVICES AND PRICES

CLIN 0005

The CLIN extended description has changed from:

Vortex Mixer, 100/240V 50/60Hz, cord set with various plugs, (i.e. CEE7 or Schuko), with speed 0 to 3,000 rpm, and load bearing capacity of up to 0.5 Kg.Unit Price to include Installation and Training by Manufacturer trained technician with, at a minimum, 2 years experience to be on site, no later than 15 working days from notification of arrival of the equipment at destination.Submit Estimated Delivery Date of Traceable Shipment, door-to door to:University of Niš Faculty of Medicine Blvd. Dr Zorana Djindjica 8118108 NisRepublic of Serbia

To:

Vortex Mixer, 100/240V 50/60Hz, cord set with various plugs, (i.e. CEE7 or Schuko), with speed 0 to 3,000 rpm, and load bearing capacity of up to 0.5 Kg. Total weight of supporting plate with vials should be 0,5Kg regardless ofthe number of vials.Unit Price to include Installation and Training by Manufacturer trained technician with, at a minimum, 2 years experience to be on site, no later than 15 working days from notification of arrival of the equipment at destination.Submit Estimated Delivery Date of Traceable Shipment, door-to door to:University of Niš Faculty of Medicine Blvd. Dr Zorana Djindjica 8118108 NisRepublic of Serbia

The following have been modified:

52.212-2 EVALUATION--COMMERCIAL ITEMS (OCT 2014)

Paragraph (a) is hereby replaced with the following:

(a) The Government will award multiple Purchase Orders with fixed unit priced CLINs resulting from this solicitation to the responsible Quoters whose quote(s) conforming to the solicitation will be most advantageous to the Government, price and technical factors considered.

Evaluation. Awards will be made using the Lowest Price Technically Acceptable (LPTA) evaluation process.

Awards will be made to the responsible Quoters on the basis of the lowest evaluated price of quotes meeting or exceeding the acceptability standards for technical factors. The Government intends to make awards without conducting discussions. After the initial evaluation of quotations is completed, if one or more quotations are rated Acceptable Technical, the initial evaluation will become the final evaluation of quotations and award will be made without discussions to the lowest priced quotes

FACTOR 1 - Technical: The Technical Factor has eighteen (18) sub-factors. Quoters shall explain how they will comply with the requirements in the RFQ by submitting narratives that demonstrate their capability to provide required supplies, as follows:

Technical Factors Standard Standard

Acceptable Unacceptable Sub-Factor 1.

CLIN 0001- Genetic Analyzer: Stand-alone analyzer, 100/240V 50/60Hz, for Sanger sequencing (resequencing NGS confirmation, indels, heterozygote detection, minor variant detection, microbial identification, genome editing validation), as well as Fragment analysis (microsatellite analysis, compatible with MLPA™, cell line authentication), shall feature, at a minimum:

• Four (4) Capillaries

• Six (6) Dyes

• Sample Format of 96-well standard plate and standard 8-tube strips

• Integrated cartridge for minimal hands-on time (polymer, array, pump, anode buffer—all in one unit); helps reduce human error

• Sanger sequencing and fragment analysis on the same run

• Internal hard drive storage 128 GB, approximately 3,500 injections or 14,000 reactions

• Interactive touchscreen with easy-to-use interface

• Auto-calibration

• Wi-Fi or LAN connectivity

• Web-enabled remote monitoring and control capability

• Tracking of consumables usage

• Universal all-in-one cartridge—unique functionality integrates POP-1 polymer, anode buffer, a polymer delivery system, and a capillary array; this novel system design allows for an on-instrument reagent life of four to six months (depending on the cartridge reaction size).

Evaluation Criteria - Cont’d.

Sub-Factor 2.

CLIN 0002- PCR System: PCR System, 100/240V 50/60Hz, to include external computer tower, software with variety of plate setup wizards, standard curve dilution and master mix recipe calculators, QC flags, data filters, and email notification, shall feature, at a minimum:

• Capacity of 1 x 96-well plate (Fast),12 x 8-tube strips

• 9 logs of linear dynamic range

• CCD Camera,

• Five excitation and five emission filters

• Halogen light source

• Thermal uniformity at top speeds.

• Ramp rates ± 3.5°C∕sec, Standard mode: ± 1.6°C∕sec

• Optical plates ensuring precision in 10-30 ul reaction volumes.

• TaqMan PCR to run multiple assays on one plate. (4 to 5 96-well plates per day)

• Capability to finish more runs per day

• Capability to distinguish between 5,000–10,000 genome equivalents (two-fold copy number difference) with 99.7% confidence

• Dyes: SYBR® Green I, FAM™, VIC™, JOE™, NED™, TAMRA™, ROX™, Texas Red®, Cy3™, Cy5™

Sub-Factor 3.

CLIN 0003- UV-VIS spectrophotometer: Standalone UV-VIS spectrophotometer, 100/240V 50/60Hz, with on-board control and appropriate software, for quantification of micro-volumes DNA and RNA, shall feature, at a minimum:

• Measurement of DNA concentration and purity without the use of additional reagents or additional manipulation of an isolated DNA sample

• Capability to measure DNA and RNA purification without the need for sample dilution.

• Screen with sliders allowing to move from right to left and vice versa as well as tilting the screen forward

• Capability to automatically identify contamination in the sample by displaying the corrected concentration

• Capability to transfer data via USB, Internet, R

• Reagent kit for verification and calibration of instrument

• Cleaning kit for appliances

• Variable pipette for measuring range of instrument

Sub-Factor 4.

CLIN 0004- Benchtop Centrifuge: Benchtop Centrifuge, 100/240V 50/60Hz, with 2 Acceleration/Deceleration profiles (standard and soft), shall feature, at a minimum:

• Direct, brushless induction driving system

• 3 direct storage program buttons.

• Swinging Bucket rotor

• Biocontainment Lids for Round Buckets (set of 4)

• Adapters for Swinging Bucket, 50 mL Conical or Skirted Tube Adapter for round buckets (set of 4)

• Adapters for Swinging Bucket, 10 mL Blood Collection Tube Adapter for round buckets (set of 4)

• Adapters for Swinging Bucket, 5/7 mL Blood Collection Tube Adapter for round buckets

• Adapters for Swinging Bucket, 3/5 mL Blood Collection Tube Adapter for round buckets

• M10 Microplate Swinging Bucket Rotor

• Sealed Buckets for M10 Microplate Swinging Bucket Rotor (set of 2)

Sub-Factor 5.

CLIN 0005- Vortex Mixer: Vortex Mixer, 100/240V 50/60Hz, cord set with various plugs, (i.e. CEE7 or Schuko), with speed 0 to 3,000 rpm, and load bearing capacity of up to 0.5 Kg, Total weight of supporting plate with vials should be 0.5 Kg, regardless of the number of vials.

Domenico.Maddaloni Highlight

Sub-Factor 6.

CLIN 0006- Laboratory Pipette: Pipettes kit to include, at a minimum:

• Four Pipetters from 0.2 to 1000μL:

- Volume Single Channel Pipette

- 0.2-2μL

- 2-20μL univ

- 20-200μL

- 100-1000μL

• Flex tips: 10, 12 x 96; 200, 2 x 96; 1000, 1 x 96

• F-stand

• Laboratory Pipetting Guide

• Reagent reservoir demo pack

- Multichannel Pipette, 8 channel, 1 to 10 μL

- Multichannel Pipette, 8 channel, 5 to 50 μL

- Multichannel Pipette, 8 channel, 30 to 300 μL

Sub-Factor 7.

CLIN 0007- Thermal Cycler: Thermal Cycler, 100/240V 50/60Hz, safe heated lid and a large LCD colour touch screen, shall feature, at a minimum:

• Blocks to optimize 3 independent PCR temperature zones

• WiFi-enabled for remote monitoring

• PCR mobile application

• USB drive to back up data

• Thermal simulation modes to replicate other PCR experiments

• Library of simulation modes

Sub-Factor 8.

CLIN 0008- Ultrasonic Cleaner: Bolt Clamped Langevin Type Transducer, Ultrasonic Cleaner 2 L, 100/240V 50/60Hz, shall feature, at a minimum:

• Bath Volume (2L / 0.07 cu.ft)

• Ultrasonic Power 100

• Bath Internal 142W x 132L, 104D

Sub-Factor 9.

CLIN 0009- Mini Incubator: Mini Incubator (10L), 100/240V 50/60Hz, to include two (2) wire shelves, shall feature, at a minimum:

• Wire shelves for space saving of inner chamber.

• Maximum 4 wire shelves supporting 8 microplates suitable for ELISA.

• Temperature range: from ambient +5 to 65

• Door opening alarm

• Microprocessor PID feedback control of the heater and the fan ensuring temperature uniformity.

• Automatic temperature calibration.

• VFD (vacuum fluorescent display), responsive touch buttons.

Sub-Factor 10.

CLIN 0010- Multiplex Flow Analyzer: Multiplex Flow Analyzer, 100/240V 50/60Hz, with Multiplexing capability of 500 unique bead regions and Compatible Assays: LABType SSO, LABType HD, LABType CWD, LABType XR, and LABScreen, shall feature, at a minimum:

• USB 2.0-compatible communications link for fast data transfer.

• Input voltage range: 100 V~ to 120 V~, 6.0 A, 50 Hz to 60 Hz or, 200 V~ to 240 V~, 3.0 A, 50 Hz to 60 Hz.

• Optics:

• Reporter channel detection: A/D resolution 16 bits.

• Reporter channel dynamic range: ≥ 4.5 decades of detection (verified with beads dyed with high levels of organic dyes).

• Reporter laser: 532 nm, nominal output 15.0+5%/-0% mW, diode-pumped; mode of operation, continuous wave (CW); maximum output power 50 mW.

• Classification laser: 638 nm, nominal output 12.0 mW to 12.5 mW, diode; mode of operation, continuous wave (CW); maximum output power 15 mW.

• Reporter detector: Photomultiplier tube, detection bandwidth of 565 nm to 585 nm.

• Classification detector: Avalanche photo diodes with temperature compensation.

• Doublet discrimination detector: Avalanche photo diodes with temperature.

Compensation.

• Fluidics:

• Dual sample fluidics paths.

• Cuvette: 200 micron square flow channel.

• Sample injection rate: 2 μL/second.

• Sample uptake volume: 10 μL to 200 μL.

• Sheath flow rate: 7.9 (+/- 0.9) mL/minute.

• Sheath pressure: 8 psi to 13 psi for normal operations; 15 psi maximum.

• Microspheres:

• Capability to distinguish 1 to 500 unique xMAP® microspheres in a single sample

• Classification of xMAP microspheres: ≥ 80%

• Total system misclassification of xMAP microspheres: ≤ 4%

• Internal sample carryover: < 1.5%

• Capability to detect a minimum of 500 fluorochromes of Phycoerythrin (PE) per xMAP microsphere

• Capability to detect and distinguishs surface reporter fluorescence emissions at 575 nm on the surface of 1 to 500 unique xMAP microspheres in a single sample

• Soluble background fluorescence emission at 575 nm automatically subtracted from fluorescence intensity values.

• Microtiter Plates

• Compatibility with 96-well plates and 384-well plates with standard width (85.6mm) and length (127.9mm), with a flat, conical or rounded bottom or with a filter at the bottom.

• Read time: 96-well plate in ~20min (up to 144.000 test/hour); 384-well plate in ~75 min (up to 153.600 tests/hour)

• Acquisition Software:

• Programmable and automated maintenance routines;

• Customizable import/export options;

• Low bead count detection/alerts and remote web monitoring;

• LIS connectivity.

Sub-Factor 11.

CLIN 0011- Automated parallel gel electrophoresis system: Benchtop Gel Electrophoresis system, 100/240V 50/60Hz, to include software, electronic user information (USB flash drive), accessories (vortex mixer, tube strip holder, tip waste bucket, USB cable), consumables (optical tube strips and caps, loading tips, test tape), user information and protocols, shall feature, at a minimum:

• Analysis of DNA and RNA samples and automatic data extraction and presentation in the analysis software

• Capability of self-maintenance in combination with advanced system diagnostics.

• Scalable Throughput: disposable consumable consisting of 16 individual lanes.

• Variable throughput from 1-16 samples.

• Compatibility with 8-tube strips.

• Maximum capacity two 8-tube strips.

• Results availability within1-2 minutes per sample

• Pre-packaged consumables ready-to-use with no required set up or priming

• Data analysis software automatically launched with sizing, quantity and integrity information

• Capability to provide RNA QC − analysis of eukaryote or prokaryote total RNA quality metric, verified with more than 300 eukaryotic and 200 prokaryotic.

• Capability to provide Genomic DNA samples with the DNA quality metric for the assessment of input genomic DNA integrity from multiple sources including degraded genomic DNA samples isolated from FFPE tissue.

• Sizing range: 35-60,000bp (DNA applications).

• Sensitivity: 5pg/µl for DNA applications, 100pg/µl for RNA applications.

Sub-Factor 12.

CLIN 0012- Microplate Reader: Multi-Mode Microplate Reader with injectors, 10” touchscreen, imbedded PC, 100/240V 50/60Hz, to include 2 injectors for flash kinetics for Absorbance, Fluorescence, and Luminescence, shall feature, at a minimum:

• Integrated Shaking and Temperature Control

• System accessories kit including reagents bottle, adaptor modules and waste tray.

• Compatibility with Micro-Volume Microplate and cuvette adapter for up to 4 cuvettes.

• NFC communication to pull up protocols created and stored on the instrument

• Cooled PMT technology for enhanced sensitivity for luminescence based applications.

• Capability to push data via software tool for quick synchronization.

• Capability for network set up

• Capability to read 6- 12- 24- 48- 96- 384- well microplates without additional adapters.

• Compatibility with cuvette adapter for up to 4 cuvettes at one time

• Compatibility with micro-volume adapter for 64 - 2µl ultra-micro samples

• Accessory-free sample path length measurement

• Label-free testing of liquid handling devices

• Detection and correction of pipetting errors

• Expansion of dynamic range of assays up to 9OD.

• Performance of spectral optimization

• Capability to find excitation and emission wavelengths for optimum signal window

• Automated way of determining Signal to Noise for assays

Sub-Factor 13.

CLIN 0013- Laboratory Freezers: Laboratory Freezers, 100/240V 50/60Hz

1x Laboratory Freezer, Control Unit with voltage free contact, alarms and offset function, Ventilated air distribution system, Defrost system, Automatic smart defrost with re-evaporation of defrost water, shall feature, at a minimum:

- Temperature range: +2°C/+20°C

- Ambient temperature range: Solid door 10°C/35°C; glass door 10°C/32°C

- Acoustic and visual High/Low temperature alarms and door alarm

- Material interior: ABS lining

- Dimensions (W x D x H): 595 x 640 x 801/1001*

- Net volume: 104 litres

- 7 shelves, 4 drawers or 2 wire baskets

- Refrigerant: R600a or R134a

- CO2e: R134a: 114

- Refrigeration capacity at -10°C: R600a 154 watt; R134a 150 watt

- Connection load (Watt) (R600a): Solid door: 99 / Glass door: 103

- Connection load (Watt) (R134a): Solid door: 106,4 / Glass door: 167

- Sound level: 36.5 dB(A)

- Ventilated air distribution system

- Automatic smart defrost with re-evaporation of defrost water

Sub-Factor 14.

CLIN 0013- Laboratory Freezers: Laboratory Freezers, 100/240V 50/60Hz

1x Laboratory Freezer: Control Unit with voltage free contact, alarms and offset function, Ventilated air distribution system, Defrost system, Automatic smart defrost with re-evaporation of defrost water, shall feature, at a minimum:

- Temperature range: -25°C/-5°C

- Ambient temperature range: 10°C/35°C;

- Acoustic and visual High/Low temperature alarms and door alarm

- Material interior: ABS lining

- Dimensions (W x D x H): 595 x 640 x 801/1001*

- Net volume: 104 litres

- 7 shelves, 4 drawers or 2 wire baskets

- Refrigerant: R600a or R134a

- CO2e: R134a: 100

- Refrigeration capacity at -25°C: R600a 156 watt; R134a 149 watt

- Connection load (Watt) (R600a): 133

- Connection load (Watt) (R134a): 142

- Sound level: 39.7 dB(A)

Sub-Factor 15.

CLIN 0013- Laboratory Freezers: Laboratory Freezers, 100/240V 50/60Hz

1x Laboratory Freezer: Control Unit with voltage free contact, alarms and offset function, Ventilated air distribution system, Defrost system, Automatic smart defrost with re-evaporation of defrost water, shall feature, at a minimum:

• Temperature range: -25°C/-5°C

• Ambient temperature range: 10°C/35°C;

• Acoustic and visual High/Low temperature alarms and door alarm

• Material interior: ABS lining

• Dimensions (W x D x H): 595 x 640 x 1776/1976*

• Net volume: 312 litres

• 25 shelves, 13 drawers and 7 wire baskets

• Refrigerant: R600a or R134a

• CO2e: R134a: 136

• Refrigeration capacity at -25°C: R600a 204 Watt, R134a 216 Watt

• Connection load (Watt) (R600a): 157

• Connection load (Watt) (R134a): 186

• Sound level: 38,6 dB(A)

Sub-Factor 16.

CLIN 0013- Laboratory Freezers: Laboratory Freezers, 100/240V 50/60Hz

1x Laboratory Freezer: 1Compressors, Acoustic alarm, Power failure alarm, Adjustable high/low temperature alarm, Open door alarm, Probe failure alarm, Alarm display as text, Contact for remote alarm, shall feature, at a minimum:

• Temperature range: -86°C/-40°C

• Max Ambient temperature (ºC) 25

• Acoustic and visual High/Low temperature alarms and door alarm

• Material interior: Stainless Steel

• Interior Dimensions (W x D x H): 360 x 493 x 600

• Net volume: 106 litres

• GSM alarm module Yes

• Battery back-up for alarms and temperature display in the event of power cut 72 hours

• Porthole for installation of PT100/CO2 back up

• Digital display

• Data logger

• RS 232 interface

• RS 485 interface

• Castors

• Refrigerant EP88

Sub-Factor 17.

CLIN 0014- Biology Microscope: Light Biology Microscope, 100/240V 50/60Hz, shall feature, at a minimum:

• Integrated Power supply

• Full Koehler Illumination

• Integrated LED illumination

• Height adjustable (two positions) trinocular tube

• 6 position nosepiece coded with light manager and eco mode function

• Mechanical stage - hard coated, Reckless, Telescopic drive, Adjustable torque

• Universal specimen stage

• Abbe condenser 0.9/1,25

• eyepiece 16x/16

• objective 10x/0.25

Sub-Factor 18.

CLIN 0015- Laboratory Mini Centrifuge: Laboratory Mini Centrifuge, 100/240V 50/60Hz, to accommodate PCR tubes, microtubes and cryotubes (0.2 to 2.0 ml), shall feature, at a minimum:

• 12 microtubes or 10 cryotubes can be run up to 13,500 rpm (12,300 xg).

• Patented air-cooling to minimize noise and heat generation

• Separate "PULSE" key for quick spin

- Sound < 56 dB

• Autoclavable 12-place fixed angle micro-rotor, snap-fit plastic rotor lid and 12 adaptors for 0.2 ml PCR tubes

EVALUATION STANDARDS. The quoter’s technical quote will be evaluated using the following standards for a final rating:

ACCEPTABLE: A quote that meets the Government’s technical requirements identified in the Request for Quotation.

UNACCEPTABLE: A quote that fails to meet the Government’s technical requirements identified in the Request for Quotation.

FACTOR 2 - Price: The Government will select the quote for each CLIN that is based upon the lowest evaluated price, technically acceptable quote.

All Contract Line Item Numbers (CLINS) must be priced by the quoter. If the quoter failed to price all CLINs, the quote will not be considered for award.

(b) A quotation is not an offer and, consequently, cannot be accepted by the Government to form a binding contract. Therefore, issuance by the Government of an order in response to a supplier’s quotation does not establish a contract. The order is an offer by the Government to the supplier to buy certain supplies or services upon specified terms and conditions. A contract is established when the supplier accepts the offer.

(c) The word "quoter" shall be substituted for the word "offeror" wherever it appears in FAR 52.212-3, Alternate I and DFARS 252.212-7000.

(End of provision)

(End of Summary of Changes)