MECs_for_Notice_of_Intent.docx

Minimum Essential Characteristics All items must be compatible with the Opera QEHS system

Computing hardware must contain

· PC (Intel(R) Core 2 Duo, 4GB RAM, 500GB HDD) with Microsoft(R) Windows(R) 7 Professional

- Cell Image Analysis (CIA) server:

- IBM x3250 Server

- Operating system: Microsoft(R) Windows(R) 7 Professional 64bit

- Processor: Intel(R) Quad Core Xeon(R) X3440 2,53GHz or better

- Memory: 16 GB

- Hard drive: 2 x 1 TByte, RAID level 1

- Network interface: GBit Ethernet- runs up to 4 RMCA licenses

Software

- Must have Enhanced Acapella(R) High Content Imaging and Analysis Software

- Must easily create segmentation and cell classification using Ready-Made Building Block Solutions simply by adding the pre-configured blocks together

- Must make image segmentation and cell classification as easy as point and click with the PhenoLOGIC machine-learning plug-in

· LED Transmission must enable brightfield imaging and digital phase contrast

· Camera Lens Opera QEHS Lx Cam 1+2 Lens

· Mechanical shipping lock CCD 2+3

· Z Adjustment Adapter Camera

· Retaining ring

· ASSY Opera detection Dichro (510/568)

· Grundplatte Cube 405_640-V2

· cable set LBC cube405

· MODT laser CUBE 405-100C

· Eccentric Shutter Assembly

· cable set LBC Non-configuration specific

· Filter positioner Nipkow-sliderTPD7 MODA

· Primary dichroic mirror within the spinning disc for simultaneous 405 nm and 488 nm excitation

· Primary dichroic mirror within the spinning disc for simultaneous 405 nm, 561 nm and 640 nm excitation

· Primary dichroic mirror within the spinning disc for simultaneous 405 nm, 488 nm, 561 nm and 640 nm excitation

· Sapphire 561-150 CW OEM Laser Head

· Sapphire OEM USB Controller LP

· Adapter Controller Sapphire + OPS

· Sapphire561 mounting parts

· Bandpass filter for CCD 1, 450/50

· Bandpass filter for CCD 1, 520/35

· Bandpass filter for CCD 1, 543/22

· Bandpass filter for CCD 1, 565/40

· Bandpass filter for CCD 2, 543/22

· Bandpass filter for CCD 2, 650/40

· Laser Cube 640-100C

· 640nm Laser clean up Upgrade set

· Image resolution of 250 nM and camera pixel size of 110 nM

· Automated 4x Magnification Unit, Air Air objective, 0.16NA, WD = 13 mm (field of view: approx. 2219 x 1677 μm²) Adaptation to Opera objective handling system