REQ___VS5012580-NOTICE_OF_INTENT-MACROGEN_CLINICAL_LABORATORY-wHOLE_-GENOME_SEQUENCING__SERVICE.pdf

NOTICE OF INTENT TO AWARD A SOLE SOURCE CONTRACT

REQUISITION NO:VS5012580

INTENDED SOURCE: Macrogen Clinical Laboratory, Inc.

PERIOD OF PERFORMANCE: 1 Year, ARO

DESCRIPTION OF REQUIREMENT:

This contract action is for the acquisition of services for which the Government intends to solicit and negotiate with only one source under the authority of Federal Acquisition Regulation (FAR)

Subpart 13.106(b)(1). The National Institute of Environmental Health Science (NIEHS), Office of Acquisitions (OA) intends to award a single source purchase order to Macrogen Clinical

Laboratory, Inc., 1330 Piccard Drive, Ste 103, Rockville, MD 20850, for the purchase of Whole

Genome Sequencing on HiSeq X, 60X Sequencing Services.

The Whole Genome Sequencing on HiSeq X, 60X Sequencing will be procured to identify molecular mechanisms underlying somatic genome instability in humans. Genomic DNA derived from blood and from clones of somatic cells will be whole-genome sequenced at high coverage (50-60x) (n=14). Somatic point mutations, insertions/deletions, and copy number alterations will be identified by comparing reads from blood with reads from somatic clone from the same individual. The Mechanisms of Genome Dynamics Group (MGDG) of the NIEHS will assess genetic changes found in somatic cells of human individuals identified through NIEHS

Environmental Polymorphisms Registry.

Whole Genome Sequencing will be performed on DNA from blood serving as a surrogate for germline and on DNA from clones of skin fibroblasts insolated from biopsies by The NIEHS

Clinical Research Unit (CRU). The ability to maintain continuity of experimental procedures is crucial to the success of the Division of Intramural Research Group at the NIEHS, RTP, NC. It has been determined that Macrogen Clinical Laboratory, Inc., is the only known responsible source capable of providing this service and no other service will satisfy the agency's requirements.

Description of Item: Quantity:

1. Sequencing HiSeq X, 60X Sequencing 15

2. Data Handling and Handling 15

The applicable North American Industry Classification System (NAICS) code for this acquisition is 541990 “All Other Professional, Scientific and Technical Services” and a Size

Standard of 15.0 M.

NIEHS will solicit and negotiate directly with Macrogen Clinical Laboratory, Inc., and no solicitation will be issued. This notice of intent is not a request for competitive proposals or quotations; however, all responsible sources may submit a bid, proposal, or quotation which shall be considered by the agency. Offerors must have an active registration in the System for Award

Management (SAM) www.sam.gov. A determination by the Government not to compete this proposed requirement based on responses to this notice is solely within the discretion of the

Government. Interested parties must express their interest and must be submitted in writing via email to Valarie K. Sims at sims3@niehs.nih.gov and are due by 10:00 AM on August 13, 2018.

No faxed documents will be accepted. If no responsive statements are received by the due date, an award will be made to Macrogen Clinical Laboratory, Inc.

http://www.sam.gov/ mailto:sims3@niehs.nih.gov