Trimedlure_Specs_12-17-2024.pdf

SPECIFICATIONS FOR TRIMEDLURE SOLID DISPENSERS

California Department of Food and Agriculture (12/17/2024)

1. SCOPE AND SPECIFICATIONS

1.1 Scope:

The dispensers shall be formulated so as to constitute a contiolled release delivery system utilizing pure Trimedlure (TML).

1.2 Specifications:

The solid dispensers shall meet the following specifications:

1. Contain a minimum of 2 grams of trimedlure*.

2. Be free of liquid trimedlure on the unit surface.

3. Be nonfriable and resistant to damage during normal field operations.

4. Be resistant to moisture.

5. Be easily and quickly installed, with minimum contamination of the dispenser from the trap adhesive, when used in a Jackson plastic bait basket and/or Champ trap.

Samples of the plastic bait basket and Champ trap are available upon request.

6. Under normal handling, shipping and deployment the dispenser shall maintain its shape and not crumble, break or shatter.

7. Once installed, dispenser must not be easily dislodged from the trap.

8. Be prepared from common, nontoxic commercial material.

9. Be capable of being stored at 20°C for one year with no reduction in efficacy.

*Trimedlure solid dispensers must meet the specifications as outlined in this document dated December 17, 2024.

2. PERFORMANCE SPECIFICATIONS

2.1 Chemical Assay:

Following field aging of production-lot dispensers, the residual lure content shall be at least 1 gram after 1 week and 0.4 gram after 6 weeks of aging; the release rates of trimedlure at 28°C in a laboratory test shall be at least 0. 7 milligrams per hour per dispenser after 1 week and 0.4 milligrams per hour per dispenser after 6 weeks aging. Reference dispensers of known quality (previously tested and approved) will be analyzed with each test as a control.

2.2 Bioassay:

Following field aging of production-lot dispensers, for 1 and 6 weeks, the number of Medflies captured shall be at least 50 percent of that caught by standard freshly baited Richmond cotton wick baited with

2.0 milliliter of the same bulk trimedlure as used for the dispensers .

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4. In the morning, place plugs one to a chamber. Adjust gas glow to 100 ml/min.

5. After 1 hour of equilibration in the chamber, remove the plug and weigh. Return the plug to the chamber.

6. The plugs are reweighed every 2 hours. The average weight loss per hour over a 6-hour period is considered the emission rate.

4.5 Procedure for Testing Trimedlure Extraction

1. The plug used for emission rate should not be used for extraction.

2. Drop the in one pint jar and 200 ml 50/50 acetone/he�ne. Allow the plug to leach at room temperature for at least 48 hours, with intermittent mixing.

2. Using 3 ml volumetric pipette transfer 3 ml aliquot to a 100 ml volumetric flask and dilute to the mark with acetone/hexane mixture.

4. Samples are ready for analysis by GC/FID.

Five micro liters (ul) of each sample are injected onto a GC/FID after calibration with known concentration of "C" isomer.

4.6 Suggested Gas Chromatography Parameters

(GC/FID)

1) Column: RTX-1 mega bore, 30 m x 0.53 ID, 1.5-micron film thickness.

2) Flow rate:

3) Temperature:

Helium Hydrogen Air

Detector Injector

10.6 mil/min

33.4 mil/min 240 mil/min

2?s c

22s c

Oven 100° C, for 1 minute, rate 10 C/min to 220 °C for 3 minutes.

4. 7 Trimedlure Content Calculation = (A)(C)(D/B)

Where: A=Total peak area (PAU) for the two Trimedlure peaks in the sample.

B= PAU for the "C" isomer the standard C=Standard concentration in mg.

D=Dilution factor

4.8 Government Testing of First Article

The State of California reserves the right to perform random testing of subsequent deliveries.

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6.0 SHIPPING, BILLING, PAYMENT INSTRUCTIONS

6.1 Shipping Address and Billing Address

California Department of Food and Agriculture Pest Detection/Emergency Projects 2423 E. Winston Rd.

Anaheim, CA 92806

(714) 520-6866 Attn: Adrian Gonzales

Hours: 7:00AM to 2:30PM, Monday through Thursday, closed during lunch (12 to 1 pm)

6.2 Payment:

Payment for the TML dispensers will be made by CDFA after the TML dispensers have been successfully bioassayed. The TML dispensers will be accepted by CDFA upon delivery. Within 60 calendar days after receipt of the TML dispensers, CDFA will complete a bioassay to detennine if the TML dispensers perform as stated above. If the TML dispensers fail to perform satisfactorily, CDFA will contact the vendor in writing to arrange for disposition of the shipment.

If the TML dispensers do not perform as required the vendor may either:

a) Furnish another lot of TML dispensers for testing using TML purchased by the vendor, or

b) If the shipment fails bioassay and must be returned, the vendor will pay all shipping costs to return the shipment and pay all cost to ship any new shipment of TML dispensers.

Questions regarding delivery and billing of the shipment may be directed to the above addresses and individual. Technical questions regarding the TML dispensers should be directed to Jason Leathers at the following:

California Department of Food and Agriculture Pest Detection/Emergency Projects 1220 N Street, 2nd Floor Sacramento, CA 95814

(916) 654-1211

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