Technical specifications - OpenSPR-XT.pdf

Technical Specifications – OpenSPR-XT

Compatible with:

Proteins / Lipids / Carbohydrates / Antibodies / Nucleic acids / Aptamers / Small molecules / Cells /

Viruses / Crude samples / Polymers / Nanoparticles

Applications include:

Kinetics Evaluation (kon, koff, KD) / Affinity (EC50) / Competition assays / Target identification / Epitope mapping / Screening / Yes - No binding / Concentration

Specifications of OpenSPR-XT:

1 Instrument Size 28 x 48 x 23 cm

2 Sensors Nanogold

3 Flow Cell PDMS, 450 nL

4 Pump rate 5 – 200 μL/min

5 Limit of Detection 1.0 pg/mm2

6 Sensitivity 6 RU/pg/mm2

7 Automation Fully Automated

8 Sample format – automatic 96/384 plates, 2/10ml vials

9 Sample capacity Any two of the above

10 Buffers and waste Buffer1, Buffer 2, DI, waste

11 Sample tray cooling - 4 oC

12 RI Resolution 10-6 RIU

13 RI Drift per 5 min 10-5 RIU

14 Temp Sensitivity 10-5 RIU/oC

15 Temp precision +/- 0.1 oC

16 Sample volume 15 – 100 – 500 µL

17 Sensor Temperature 10 to 40 oC

18 Channels 2 channels

19 Reference Channel Yes

20 Concentration Range 0.1nM – 1 mM

21 Analysis Software TraceDrawer

22 File output CSV, TraceDrawer

23 Pump rate 5-200 ul / min

24 Affinity Range pM-mM

25 Sensors Plain Gold, NTA, Biotin, Carboxyl, Amine, Liposome (LIP), GST, Thiol, Hydrophobic (MEM), Protein A, Streptavidin, High sensitivity (COOH, NTA, Streptavidin)

26 Lowest detectable molecular weight 5Da