StatementOfWork - LabServicesUpdate.docx
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- RNA and DNA Sequencing
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- 12405B21-1039972S
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Statement of work Work to be performed:
The proposed work to be performed includes USDA-ARS Genetics and Sustainable Agriculture Unit shipping of experimental samples (DNA extracted from soil) to the contractor/lab service provider. Libraries from the DNA from these samples will be prepared by the contractor/lab service provider. A total of 200 samples will be sequenced for 16S rRNA sequencing at 2 x 300 bp paired end reads, while 150 samples will be sequenced for shallow shotgun metagenomic sequencing, with approximate 0.5 to 2 million reads per sample. The contractor/lab service provider shall analyze the samples and provide raw data for 16S sequencing and shallow shotgun metagenomic sequencing but will also provide further bioinformatics for shallow shotgun metagenomic sequencing. Sequencing must be conducted on an Illumina Next Seq (2x150bp).
This sequencing must be able to fit into our ongoing research and sequencing that has been conducted by Mircobiome Insights.
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