Statement of Work Sugarcane Chromosome Karyotype Analysis.docx
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- Custom Chromosomes Karyotype Analysis and FISH Service Federal contract opportunity
- Solicitation number
- 12405B24Q0037
About this file
This statement of work outlines requirements for a custom chromosomes karyotype analysis and fluorescence in situ hybridization service for the United States Department of Agriculture Agricultural Research Service. The contractor shall perform cytological analysis on sugarcane parents to determine their ploidy levels and identify molecular markers using high-resolution metaphase chromosome preparation technology and fluorescence in situ hybridization. The contractor will count chromosome numbers, develop FISH for parents, and provide data and analytics. Materials including sugarcane knots and genome DNA from one genotype will be provided by the customer. Deliverables will include a detailed report of methods and results as well as the complete raw data set. The point of contact is Aliya Momotaz and the period of performance is within 90 days of award.
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| File | Type | Posted |
|---|---|---|
| Clauses FAC 2023-06 eff. 12-04-23 under $250k (Solicitation).pdf |
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Text version
UNITED STATES DEPARTMENT OF AGRICULTURE
AGRICULTURAL RESEARCH SERVICE
Sugarcane Field Station
STATEMENT OF WORK
Sugarcane Chromosome Karyotype Analysis
Introduction: As part of the sugarcane breeding activities at the Sugarcane field station in Canal Point, parental fertility is very important to produce high quality viable seeds. Since sugarcane is highly polyploid with aneuploidy, it would be beneficial to have the parent’s cytological information available.
Scope: The Contractor shall perform the cytological analysis on sugarcane parents to determine their ploidy levels and identify molecular markers.
Technical Requirements:
· high-resolution metaphase chromosome preparation technology
· fluorescence in situ hybridization
Point of Contact: Aliya Momotaz, (561) 924-5227, Aliya.Momotaz@usda.gov
Period of Performance: Within 90 days after award.
Government Furnished: Access to the facility during normal business operations (Monday through Friday, 7:30 am to 4 pm)
Performance Requirement Measures:
· Count chromosome numbers using high-resolutions chromosome preparation technique.
· Develop Fluorescence in situ hybridization for parents.
· Provide data and analytics.
Service Description:
Chromosomes karyotype analysis and FISH
• Material: Sugarcane knots to be provided by customer (10 sugarcane knots will be great, if not, provide as many as possible)
• Root tips will be collected from the germinated sugarcane knots• Probe: Genome DNA from one genotype
- Genome DNA will be extracted from the roots
• Deliverable
- Detailed report of methods and results;
- Complete raw data set;
File details come from the government source that posted it. Updated .