Statement_of_Work_shotgun_Davies_06162025.docx
DOCX document 17 KB Posted
- Solicitation number
- 1232SA25Q0901
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This document is a Statement of Work (SOW) for metagenomic sequencing of 192 pig fecal and digesta samples issued by the USDA Agricultural Research Service (ARS), Animal Biosciences and Biotechnology Laboratory. The SOW requires a contractor to perform shotgun sequencing of DNA samples, producing approximately 20 million read pairs per sample using Illumina Flex technology and the NovaSeq X Plus sequencer. Deliverables include raw sequence reads in fasta or fastq format for each sample.
The associated federal contract opportunity (Solicitation Number 1232SA25Q0901) is a combined synopsis/solicitation for commercial items under NAICS code 541714 with a small business size standard of 1,000 employees. The government anticipates awarding a Firm Fixed Price contract using Lowest Price Technically Acceptable (LPTA) evaluation methodology. Vendors must submit quotes including technical specifications and pricing, with evaluation focused on meeting minimum standards in the Statement of Work and lowest price considerations.
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STATEMENT OF WORK
Metagenomic Sequencing of 192 Pig Fecal and Digesta Samples Background USDA, Agricultural Research Service (ARS), Animal Biosciences and Biotechnology Laboratory (ABBL) requests a Contractor from which to purchase a service which will produce a set of shotgun sequencing reads for 192 pig fecal and digesta samples from along the gastrointestinal tract. Shotgun sequencing is the sequencing of all DNA present in a sample. here, it is the sequencing of all microbial DNA in piglet fecal and digesta samples. ARS scientists will use these data in various downstream bioinformatics analyses including the assignment of sequences to species to determine the taxonomic composition of the samples, and the identification of genes and pathways each species contains. Production of this highly dimensional dataset will result in outcomes which will contribute to the meeting of multiple National Program 101 objectives.
Statement of Work 192 DNA samples will be provided by ARS-ABBL scientists to the Contractor, who will in turn provide shotgun metagenomic library preparation and sequencing on these samples (~20 million read pairs per sample, PE 2 X 150). DNA should be quantified using the Qubit system, normalized, and then processed into sequencing libraries using Illumina Flex technology and sequenced on the Illumina NovaSeq X Plus sequencer. Raw sequence data for each sample will be delivered to researchers within ARS-ABBL.
Schedule of Line Items Line Item 01 –library preparation and sequencing of 192 piglet fecal samples Deliverable will include raw sequence reads in fasta or fastq format for each of 192 piglet fecal and digesta samples
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