Statement of Work for Quotes NFFP71002102786.docx

STATEMENT OF NEED FOR SUPPLY OR SERVICE

NFFP71002102786

1.0 GENERAL

1.1 BACKGROUND

The Northwest Fisheries Science Center (NWFSC), of the National Marine Fisheries Service (NMFS or NOAA-Fisheries), National Oceanic and Atmospheric Administration (NOAA), Department of Commerce (DOC) is a government agency charged with the mission of stewardship of living marine resources.

1.2 OBJECTIVE

This contract will require the following products and services: 1) DNA extraction from up to 4,896 Chinook salmon fin clip tissue samples, 2) DNA sequencing of for an amplicon panel provided to the vendor, and 3) DNA analysis and production of genotypes.

2.0 TECHNICAL SPECIFICATIONS

2.1 Skills Required

The vendor shall meet the following requirements:

2.1.1 The vendor will have the ability to isolate and sequence DNA from Chinook salmon tissue samples (amplicon sequencing from fin clips).

2.1.2 The vendor will have staff trained in library preparation, data processing, and recording sufficient to achieve the goals of Section 2.2.

2.1.3 The vendor will be expert in analysis of next-generation DNA sequence data and specifically in the genotyping of Chinook salmon for standardized single-nucleotide polymorphisms from a user-supplied amplicon panel (“GTseq” with the “Chinook 299 Panel”).

3.0 DELIVERABLES & SCHEDULE

The vendor will provide the following products and services to support NOAA’s objectives, as specified in Section 2 of this Statement of Need:

3.1 DNA sequence files (text files in FASTQ format), de-multiplexed for analysis

3.2 Multilocus genotypes in CSV format for each individual tissue sample submitted (up to 4,896)

3.3 Samples will be processed and data delivered on a schedule that is consistent with the workflow requirements of the vendor but within the PERIOD OF PERFORMACE described below

4.0 PERIOD OF PERFORMANCE

The period of performance for this contract shall not exceed 31 Dec 2022

5.0 PLACE OF PERFORMANCE

The primary place of performance will be the Contractor’s facilities.

6.0 POINT OF CONTACT

To be provided at time of award.

7.0 SECURITY

· Personnel performing work must wear visible identification at all times when physically within Government facilities or on Government premises.

· Personnel performing work will not work on-site.

· Personnel performing work will not require access to NOAA facilities.

· Personnel performing work will not require a PIV or CAC.

· Personnel performing work will not require a NOAA email address.

· Personnel performing work will not require use of DOC owned IT Equipment (Laptop, Desktop Computer).

· Personnel performing work will not require Non-Government IT equipment to interconnect to a DOC network.

· Personnel performing work will not require access to non-public DOC/NOAA data.

8.0 DATA PRIVACY

Should work on this agreement require that personnel have access to Privacy information, personnel shall adhere to the Privacy Act, Title 5 of the U.S. Code, Section 552a and applicable agency rules and regulations.

Data created, obtained and collected during the performance of this agreement is the property of NMFS. Data may not be released by any party without written consent from the NMFS POC. Data must be protected from unauthorized disclosure. Personnel must comply with all Federal, DOC, NOAA, and NMFS policies relating to IT and data security.

9.0 NON-PERSONAL SERVICE

Personnel performing services under this order will be controlled, directed and supervised at all times by management personnel of the contractor. Personnel will perform independent of and without the supervision of any Government official. Actions of contractor employees may not be interpreted or implemented in any manner that results in any employee creating or modifying Federal policy, obligating the appropriated funds of the U.S. Government, overseeing the work of Federal employees, providing direct personal services to any Federal employee or otherwise violating the prohibitions set forth in Parts 7.5 and 37.1 of the Federal Acquisition Regulations. The NMFS will perform the inspection and acceptance of the completed work.