Statement of Work.pdf
PDF 166 KB Posted
- Attached to
- Plant Sequencing
- Solicitation number
- 1232SA25Q0430-B
About this file
This Statement of Work (SOW) details a sequencing service requirement for the United States Department of Agriculture, Agricultural Research Service's Plant Science Research Unit in Raleigh, North Carolina. The agency requires twelve (12) lanes of single-end AVITI 150 bp high-output flow cell sequencing, with specific technical specifications including: a yield of approximately 500 million reads (75 Gbp) per lane, an average base read quality score of Q40 or higher, and loading each lane with 384 pooled uniquely barcoded DNA sample libraries. Additional requirements include pre-sequencing quality control using Qubit, Tapestation, and Collibri qPCR methods, and returning sequence FASTQ files, FastQC reports, and flow cell reports via password-protected FTP within 14 days of library receipt. The sequencing platform must demonstrate equal or superior performance to meet all Statement of Work requirements.
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Text version
The United States Department of Agriculture, Agricultural Research Service, Plant Science
Research Unit, Raleigh, North Carolina requires twelve (12) lanes of single end AVITI 150 bp
High output flow cell sequencing service to be performed, including pre-sequencing quality control. The data generated from the 12 lanes of next generation sequencing will be analyzed and utilized in conjunction with the Plant Science Research Unit’s ongoing genetic investigation of complex traits of plants.
Service Requirements:
1) 12 lanes of AVITI single end 150 bp sequencing on High output flow cells with a yield of approximately 500 million reads (75 Gbp) lane and average base read quality score greater than or equal to Q40. Each lane is to be loaded with 384 pooled uniquely barcoded DNA sample libraries provided by the Plant Science Research Unit, Raleigh, NC.
2) Qubit, Tapestation, and Collibri qPCR pre-sequencing quality control for 12 AVITI lanes.
3) Return of sequence FASTQ files, FastQC reports, and flow cell reports detailing pass filter polony, Q-score, and yield statistics via password-protected FTP within 14 days of receipt of libraries.
Sequencing Platform must be demonstrated to be equal or better and able to meet or exceed all requirements of this SOW including Quality Control.
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