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8-Channel System Specifications – Animal Disease Research Unit, Pullman, WA
| Types of analysis and applications |
| Binding: Yes/No, kinetics affinity, steady-state affinity, affinity maturation, competition/cross-reactivity |
Antibody: isotyping, epitope binning Target concentration in complex biologic samples: relative and absolute
Affinity constant
(KD)
100 pM – 1 mM
| Kinetic rate ranges (minimums) |
| Association: 101 to 106 M-1sec-1 Dissociation: 10-6 to 10-1sec-1 |
| Molecular weight minimum detection limit |
| ~300 Daltons |
| Quantitation minimum |
| Sensor options should include sub-microgram per ml detection |
| Real-time minimum data acquisition rate |
| 5 Hz |
| Baseline noise maximum |
| < 3 pM RMS |
| Baseline drift maximum |
| < 40 pm/hour |
| Sample type compatibility* |
| Proteins, peptides, antibodies, DNA, RNA, small molecule, liposomes, virus, and compatible with relatively crude sample homogenates. |
| Probe-based |
| Minimum two probes |
Some probe options include regenerative technology
| Throughput |
| 8 probes in parallel, 96-well plate format minimum |
| Orbital shaking |
| Variable with a maximum of 1,500 RPM |
| Sample capacity |
| Minimum of two loaded 96 well plates |
| Sample volume |
| Minimum 200 uL (96-well plate format) |
| Integrated data acquisition and analysis (includes monitor) |
| Assay setup and Analysis templates Real-time display of results |
Algorithms for multiple binding models One-run measurement of quantitation, kinetics, and regeneration
| Probe binding methods |
| 1. Binding to biotinylated or Avi-tagged biomolecules |
2. Covalent binding through amine group of proteins
3. Binding to His-tagged proteins (antibody-based method, Ni-based method)
4. Binding to antibodies (Fc method, FAB method)
* Microfluidic flow-thru technology (SPR) is not compatible with crude homogenates, which may occlude the system and stop sample flow. Probe-based (BLI) technology is fully compatible with crude homogenates.