Statement of Work_Aug12-2025.pdf
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- Attached to
- Plant Sequencing
- Solicitation number
- 1232SA25Q0430-C
About this file
This document is a Statement of Work (SOW) from the United States Department of Agriculture's Agricultural Research Service, Plant Science Research Unit in Raleigh, North Carolina. The agency requires six high output flow cells of single-end AVITI 150 bp sequencing service, which includes pre-sequencing quality control for 12 AVITI lanes. The specific requirements include generating approximately 500 million reads (75 Gbp) per lane with an average base read quality score of Q40 or higher, using 384 pooled uniquely barcoded DNA sample libraries.
The service involves performing Qubit, Tapestation, and Collibri qPCR pre-sequencing quality control, and delivering sequence FASTQ files, FastQC reports, and flow cell reports via password-protected FTP within 14 days of receiving the libraries. The generated sequencing data will support the Plant Science Research Unit's ongoing genetic investigation of complex plant traits, with each lane to be loaded with DNA samples provided by the research unit in Raleigh, North Carolina.
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Text version
The United States Department of Agriculture, Agricultural Research Service, Plant Science
Research Unit, Raleigh, North Carolina requires six (6) flow cells of single end AVITI 150 bp
High output sequencing service to be performed, including pre-sequencing quality control. The data generated from the flow cells of next generation sequencing will be analyzed and utilized in conjunction with the Plant Science Research Unit’s ongoing genetic investigation of complex traits of plants.
Service Requirements:
1) 6 high output flow cells (12 lanes) of AVITI single end 150 bp sequencing with a yield of approximately 500 million reads (75 Gbp) lane and average base read quality score greater than or equal to Q40. Each lane is to be loaded with 384 pooled uniquely barcoded DNA sample libraries provided by the Plant Science Research Unit, Raleigh, NC.
2) Qubit, Tapestation, and Collibri qPCR pre-sequencing quality control for 12 AVITI lanes.
3) Return of sequence FASTQ files, FastQC reports, and flow cell reports detailing pass filter polony, Q-score, and yield statistics via password-protected FTP within 14 days of receipt of libraries.
File details come from the government source that posted it. Updated .