statement_of_work_antibody_production_PDL1.docx

STATEMENT OF WORK

Rabbit Polyclonal Antibody production against Chicken PD-L1 IgV domain Avian Disease and Oncology Lab 5/19/2023

GENERAL

SCOPE OF WORK. This procurement is located at the USNPRC, Avian Disease and Oncology Laboratory at 4279 E. Mount Hope Rd., East Lansing, MI 48823. The requirements for this service work are defined in the Statement of Work (SOW). The contract requirements are defined in this Statement of Work (SOW).

The purpose of this SOW is to solicit bids for rabbit polyclonal antibody development with the following specifications:

· The antibody is to be developed against the extracellular IgV domain of chicken PD-L1.

· The chicken PD-L1 IgV domain sequence is to be cloned into an appropriate expression vector and purified recombinant antigen sufficient for planned rabbit immunizations is produced.

· 2 rabbits are to be immunized on a 3-immunization schedule with serum collections after the second and third immunizations sufficient to produce at least 0.1 mg of sterile filtered, affinity-purified rabbit polyclonal antibody.

· The produced rabbit polyclonal antibody is validated by both indirect ELISA and Western blot against the PD-L1 IgV domain antigen and produces a positive signal in both assays.

· Peripheral blood mononuclear cells are to be stored from 1 rabbit for 3 months for optional future use in monoclonal development.

HOURS OF OPERATION. Normal Hours. ADOL is open Monday through Friday from 8am until 4pm except for Government holidays.

GOVERNMENT-FURNISHED PROPERTY AND SERVICES

No Government-furnished property will be provided. Government-furnished utilities will be provided where available.

CONTRACTOR-FURNISHED ITEMS AND SERVICES

GENERAL. Contractor shall furnish everything required to perform this Statement of Work.

CLEAN-UP. The Contractor is responsible for cleanup of any work site.