Specifications Required.pdf
PDF 73 KB Posted
- Attached to
- High-throughput, Next-Generation Sequencer Federal contract opportunity
- Solicitation number
- RFQ-NIAID-2065848
About this file
This document contains a solicitation for a high-throughput next-generation sequencer. The National Institute of Allergy and Infectious Diseases is seeking to purchase a sequencer to increase sequencing capacity to support methodologies including bulk and single cell sequencing, targeted genomic sequencing, whole genome sequencing, metagenomics, and long-read pathogen sequencing. Quotes are due by September 2, 2021 and award will be made within 60 days. Evaluation criteria include technical approach, past performance, and price. The solicitation provides detailed minimum specifications for the sequencer, including the ability to generate over 9 terabytes of data in 24 hours using 300bp reads run in parallel on two wafers. The place of delivery is the Vaccine Research Center in Bethesda, Maryland. The award will be a firm-fixed-price contract.
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| File | Type | Posted |
|---|---|---|
| Solicitation Attachments.pdf |
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Text version
Specifications Required:
• Reliably provide a $100 per genome price that is sustainable
• Accommodate multiple runs worth of reagents and consumables
• Multiple runs with tunable read lengths that can be generated from a single sequencing cartridge
• Barcoded consumables
• Unattended operation
• Parallel sequencing of two wafers simultaneously
• Generate >9 Tb in 24 hours using 300 bp read length
• Up to 6 Tb with a 300 bp read length across two wafers in parallel
• Up to 3 Tb per 300 bp read length on a single wafer
• Utilize accurate, continuous 300bp reads to further elucidate sequencing depth
• Up to 10 billion reads and up to 3 Tb of output with a 300 bp read length on a single wafer
• Each sequencing cartridge can accommodate three 300 bp runs
• A 300 bp sequencing run can be completed in 16 hours
• Automated clonal amplification for multiple runs in parallel completed in 16 hours
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