Specifications.pdf

SPECIFICATIONS FOR TRIMEDLURE SOLID DISPENSERS

California Department of Food and Agriculture

1. SCOPE AND SPECIFICATIONS

1.1 Scope:

The dispensers shall be formulated so as to constitute a contiolled release delivery system utilizing pure Trimedlure (TML).

1.2 Specifications:

The solid dispensers shall meet the following specifications:

1. Contain a minimum of 2 grams of trimedlure*.

2. Be free of liquid trimedlure on the unit surface.

3. Be nonfriable and resistant to damage during normal field operations.

4. Be resistant to moisture.

5. Be easily and quickly installed, with minimum contamination of the dispenser from the trap adhesive, when used in a Jackson plastic bait basket and/or Champ trap.

Samples of the plastic bait basket and Champ trap are available upon request.

6. Under normal handling, shipping and deployment the dispenser shall maintain its shape and not crumble, break or shatter.

7. Once installed, dispenser must not be easily dislodged from the trap.

8. Be prepared from common, nontoxic commercial material.

9. Be capable of being stored at 20°C for one year with no reduction in efficacy.

2. PERFORMANCE SPECIFICATIONS

2.1 Chemical Assay:

Following field aging of production-lot dispensers, the residual lure content shall be at least 1 gram after 1 week and 0.4 gram after 6 weeks of aging; the release rates of trimedlure at 28°C in a laboratory test shall be at least 0. 7 milligrams per hour per dispenser after 1 week and 0.4 milligrams per hour per dispenser after 6 weeks aging. Reference dispensers of known quality (previously tested and approved) will be analyzed with each test as a control.

2.2 Bioassay:

Following field aging of production-lot dispensers, for 1 and 6 weeks, the number of Medflies captured shall be at least 50 percent of that caught by standard freshly baited Richmond cotton Vllick baited with

2.0 milliliter of the same bulk trimedlure as used for the dispensers .

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Only those products, which have been tested and approved by either CDFA or USDA for use in the Mediterranean fruit fly control program, will be considered for award.

3. TESTING PROCEDURES FOR SOLID DISPENSERS

General-The Contractor is responsible for the performance of all sampling and testing as specified herein. Except as otherwise specified, the Contractor may utilize his own or any other testing facilities and services acceptable to the State. Records of the examination and tests shall be kept complete and available to the State.

3.1 Emission rate as determined by weight loss at constant temperature:

1. Release rates are determined by weight loss in an incubator oven, Thelco Model 2, Precision Scientific, Chicago. The air is admitted to the oven through six inlet ports but is not directed within or out of the oven. Six holes are drilled through the oven wall to function as exit ports to release the air. (J. Econ. Entomol. 82:860-867, 1989).

2. Two replicate plugs of each treatment are unwrapped and left to equilibrate overnight at room temperature.

3. In the morning, the plugs (no more than 8) are then placed on the shelf in the oven which has been pre-set to 28.0 plus or minus 0.5°C and into which air is flowing at the rate of 100 ml/min. through each of the six inlet ports.

4. After 1 hour of equilibration in the oven, the plugs are removed and weighed to the nearest 0.02 mg and returned to the oven.

5. The plugs are reweighed after each of three 2-hour intervals; the average weight loss/hour over the 6 hours is considered to be the emission rate.

Emission rate can also be detennined using the NMRAL Method PRO105 (See 4.0)

3.2 Trimedlure (TML) Content Measurement:

The quantity of trimedlure in each dispenser is measured by gas chromatography on solvent extracts.

The analyses for TML content are conducted on a gas chromatograph equipped with an automatic sampler and a 30m, SPB-1 (non-polar) wide-bore (0.75 mm i.d.) capillary column (Supelco, Bellefonte, PA.).

1. Single polymeric plugs are weighed, measured (length and diameter) and then dropped into a measured volume of 50/50 acetone/hexane. Two replicates of each treatment are analyzed. The volume of solvent used for extraction varies from 100 to 800 ml depending on the expected content; then a dilution is made to achie\e a concentration about 1 ug/ul.

2. The plugs are allowed to leach at room temperature for at least 48 hours with intermittent mixing prior to analysis.

3. A solution crystalline TML-C isomer of known concentration is used as the external standard; the concentration should be in the same range as the samples, i.e. about 1 ug/ul.

4. Aliquots of samples and standards are measured for analysis. Using an auto sampler,

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1 uL injections are analyzed following these chromatographic conditions: Injector temperature - 225°C; Detector temperature - 250°C; Temperature program - initial temperature of 100 °C for 2 minutes, ramp at 10°C/minute to 250°C and hold at 250°C for 6 minutes. Total run time is 23 minutes.

5. The TML concentration is determined by comparison of the combined area of the 2 TML peaks (ca. 7 minute retention times) in the samples with the area of the single TML-C peak in the standards which bracket the samples. The total TML content of the plugs is then determined using the extraction volume in the calculation.

The GC analysis on the wide-bore column is used also to calculate the percentage of the TML which is C isomer; the first GC peak is the other trans (and many of the cis) isomers while the second peak is TML-C. The TML-C concentration is the area of the 2nd peak x 100 / total area of the 2 peaks.

NMRAL Method PRO105 is another way to measure Trimedlure content.

4. ALTERNATE TESTING PROCEDURES FOR SOLID DISPENSERS (NMRAL METHOD

PRO105.

4. 1 Reagents:

1. 50/50 Acetone/hexane (V:V) (Preparation of Reagents) (see part 4.3)

2. Acetone, distilled-in-glass

4.2 Equipment:

1. Caliper, double scale.

2. Water circulator.

3. Analytical balance.

4. Wheaton 1000 ml reaction vessel and cap.

5. 1-pint jar with Teflon lined cap.

6. 25 ml graduated cylinder.

7. 3 ml volumetric pipette

8. 100 ml volumetric flask.

9. Gas Liquid Chromatograph (GLC) equipped with Flame Ionization Detector (FID).

4.3 Preparation of reagents:

50/50 acetone/hexane (V:V) - decant 500 ml hexane and 500 ml acetone into 1000 ml graduated cylinder. Transfer to a stoppered bottle for storage.

4.4 Procedure for testing emission rate:

1. Unwrap dispenser, record the color and odor of the plugs and whether or not there are white crystals on the surface. Using double scale caliper measure the length and diameter in millimeters (mm) and record.

2. Using an. analytical balance, weigh the plug to the nearest 0.0001 grams and record the weight. Leave the plug to equilibrate overnight at room temperature.

3. Connect the vessels to the water circulator in a series. Set the water temperature to

28.0 plus or minus 0.5°C.

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4. In the morning, place plugs one to a chamber. Adjust gas glow to 100 ml/min.

5. After 1 hour of equilibration in the chamber, remove the plug and weigh. Return the plug to the chamber.

6. The plugs are reweighed every 2 hours. The average weight loss per hour over a 6-hour period is considered the emission rate.

4.5 Procedure for Testing Trimedlure Extraction

1. The plug used for emission rate should not be used for extraction.

2. Drop the in one pint jar and 200 ml 50/50 acetone/he�ne. Allow the plug to leach at room temperature for at least 48 hours, with intermittent mixing.

2. Using 3 ml volumetric pipette transfer 3 ml aliquot to a 100 ml volumetric flask and dilute to the mark with acetone/hexane mixture.

4. Samples are ready for analysis by GC/FID.

Five micro liters (ul) of each sample are injected onto a GC/FID after calibration with known concentration of "C" isomer.

4.6 Suggested Gas Chromatography Parameters

(GC/FID)

1) Column: RTX-1 mega bore, 30 m x 0.53 ID, 1.5-micron film thickness.

2) Flow rate:

3) Temperature:

Helium Hydrogen Air

Detector Injector

10.6 mil/min

33.4 mil/min 240 mil/min

2?s c

22s c

Oven 100° C, for 1 minute, rate 10 C/min to 220 °C for 3 minutes.

4. 7 Trimedlure Content Calculation = (A)(C)(D/B)

Where: A=Total peak area (PAU) for the two Trimedlure peaks in the sample.

B= PAU for the "C" isomer the standard C=Standard concentration in mg.

D=Dilution factor

4.8 Government Testing of First Article

The State of California reserves the right to perform random testing of subsequent deliveries.

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5.0 - PACKAGING, MARKING AND PRODUCTION REQUIREMENTS

5.1 Marking Deliverables

The purchase order number shall be placed on or adjacent to all exterior mailing or shipping labels of deliverable items called for by the contract.

Mark deliverables, except for reports, for:

CDFA, Plant Health and Pest Prevention Services, PD/EP

5.2 Packing For Domestic Shipment

Material shall be packed for shipment in such a manner that will insure acceptance by common carriers and safe delivery at destination. Containers and closures shall comply with the Interstate Commerce Commission regulations, Uniform Freight Classification Rules, or regulations of other carriers as applicable to the mode of transportation.

5.3 Special Packing And Marking

Two thousand individually wrapped dispensers shall be packed in cartons that will protect the contents and be of sufficient strength to allow stacking to a height of 60 inches without crushing cartons at the bottom of the stack.

All characters shall have a minimum height of 3/8 inch.

Each carton shall have the following information displayed on two outside walls in addition to other required markings:

a) Contents: Trimedlure dispensers

b) CDFA, PHPPS, PD/EP

c) Up

d) Do Not Crush

e) Store in a dry place

f) Quantity

g) Purchase Order Number

h) Date of Manufacture

i) Manufacturer's Name

j) Manufacturer's Lot Number

k) Storage Instructions

5.4 Special Packaging

Individual dispensers shall be packaged in sealed airtight, moisture-proof containers or packets. Each packet shall be clearly marked to identify contents, storage instructions, warning and/or other pertinent information regarding the safe use of contents. Individual packages shall have a permanent identification number that will support quality assurance tracking to determine manufacturer's lot or batch number.

5.5 Production Requirements

The TML dispensers shall be produced in no more than three lots.

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6.0 SHIPPING, BILLING, PAYMENT INSTRUCTIONS

6.1 Shipping Address and Billing Address

California Department of Food and Agriculture Pest Detection/Emergency Projects 2423 E. Winston Rd.

Anaheim, CA 92806

(714) 520-6866 Attn: Adrian Gonzales

Hours: 0700 to 1700, Monday through Thursday, closed during lunch (12 to 1 pm)

6.2 Payment:

Payment for the TML dispensers will be made by CDFA after the TML dispensers have been successfully bioassayed. The TML dispensers will be accepted by CDFA upon delivery. Within 60 calendar days after receipt of the TML dispensers, CDFA will complete a bioassay to detennine if the TML dispensers perform as stated above. If the TML dispensers fail to perform satisfactorily, CDFA will contact the vendor in writing to arrange for disposition of the shipment.

If the TML dispensers do not perform as required the vendor may either:

a) Furnish another lot of TML dispensers for testing using TML purchased by the vendor, or

b) If the shipment fails bioassay and must be returned, the vendor will pay all shipping costs to return the shipment and pay all cost to ship any new shipment of TML dispensers.

Questions regarding delivery and billing of the shipment may be directed to the above addresses and individuals. Technical questions regarding the TML dispensers should be directed to Jason Leathers at the following:

California Department of Food and Agriculture Pest Detection/Emergency Projects 1220 N Street, 2nd Floor Sacramento, CA 95814

(916) 654-1211

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